Brock Biology of Microorganisms (14th Edition)
14th Edition
ISBN: 9780321897398
Author: Michael T. Madigan, John M. Martinko, Kelly S. Bender, Daniel H. Buckley, David A. Stahl, Thomas Brock
Publisher: PEARSON
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Chapter 2, Problem 9RQ
Cells of Escherichia coli transport lactose via lac permease, glucose via the phosphotransferase system, and maltose via an ABC-type transporter. For each of these sugars describe: (1) the components of the transport system and (2) the source of energy that drives the transport event.
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A bacterial lactose transporter, which is highly specific for lactose, contains a Cys residue that is essential to its transport activity. Covalent reaction of N-ethylmaleimide (NEM) with this Cys residue irreversibly inactivates the transporter. A high concentration of lactose in the medium prevents inactivation by NEM, presumably by sterically protecting the Cys residue, which is in or near the lactose-binding site. You know nothing else about the transporter protein. Suggest an experiment that might allow you to determine the Mr of this Cys-containing transporter polypeptide.
Name the three classes of membrane transport proteins. Explain which one or ones of these classes is able to move glucose and which can move bicarbonate (HCO3−) against an electrochemical gradient. In the case of bicarbonate, but not glucose, the ΔG of the transport process has two terms. What are these two terms, and why does the second not apply to glucose? Why are cotransporters often referred to as examples of secondary active transport?
Name the three classes of membrane transport proteins. Explain which one or ones of these classes is able to move glucose and which can move bicarbonate (HCO3 −) against an electrochemical gradient. In the case of bicarbonate, but not glucose, the ΔG of the transport process has two terms.What are these two terms, and why does the second not apply to glucose? Why are cotransporters often referred to as examples of secondary active transport?
Chapter 2 Solutions
Brock Biology of Microorganisms (14th Edition)
Ch. 2.1 - Define the terms magnification and resolution.Ch. 2.1 - Prob. 2MQCh. 2.2 - Prob. 1MQCh. 2.2 - Prob. 2MQCh. 2.2 - How can cells be made to fluoresce?Ch. 2.3 - Prob. 1MQCh. 2.3 - Prob. 2MQCh. 2.4 - Prob. 1MQCh. 2.4 - Prob. 2MQCh. 2.5 - How do cocci and rods differ in morphology?
Ch. 2.5 - Prob. 2MQCh. 2.6 - What physical property of cells increases as cells...Ch. 2.6 - How can the small size and haploid genome of...Ch. 2.6 - What are the approximate limits to how small a...Ch. 2.7 - Prob. 1MQCh. 2.7 - Prob. 2MQCh. 2.8 - Prob. 1MQCh. 2.8 - Prob. 2MQCh. 2.9 - Compare and contrast simple transporters, the...Ch. 2.9 - Prob. 2MQCh. 2.10 - Why do bacterial cells need cell walls? Do all...Ch. 2.10 - Prob. 2MQCh. 2.10 - Prob. 3MQCh. 2.11 - Prob. 1MQCh. 2.11 - Prob. 2MQCh. 2.11 - Prob. 3MQCh. 2.11 - Prob. 4MQCh. 2.12 - Prob. 1MQCh. 2.12 - Prob. 2MQCh. 2.12 - Prob. 3MQCh. 2.13 - Prob. 1MQCh. 2.13 - Prob. 2MQCh. 2.14 - Prob. 1MQCh. 2.14 - Chapter Review Why would it be impossible for...Ch. 2.14 - Chapter Review How are magnetosomes and the...Ch. 2.15 - Prob. 1MQCh. 2.15 - Prob. 2MQCh. 2.16 - Prob. 1MQCh. 2.16 - Prob. 2MQCh. 2.16 - Prob. 3MQCh. 2.17 - Prob. 1MQCh. 2.17 - Prob. 2MQCh. 2.18 - Prob. 1MQCh. 2.18 - Prob. 2MQCh. 2.19 - Prob. 1MQCh. 2.19 - Prob. 2MQCh. 2.19 - Prob. 3MQCh. 2.19 - Chapter Review How does scotophobotaxis differ...Ch. 2.20 - Prob. 1MQCh. 2.20 - Prob. 2MQCh. 2.20 - Prob. 3MQCh. 2.21 - Prob. 1MQCh. 2.21 - Prob. 2MQCh. 2.21 - Prob. 3MQCh. 2.22 - Prob. 1MQCh. 2.22 - Prob. 2MQCh. 2.22 - Prob. 3MQCh. 2 - Prob. 1RQCh. 2 - Prob. 2RQCh. 2 - Prob. 3RQCh. 2 - What are the major morphologies of prokaryotic...Ch. 2 - How large can a bacterium be? How small? Why is it...Ch. 2 - Prob. 6RQCh. 2 - Prob. 7RQCh. 2 - Prob. 8RQCh. 2 - Cells of Escherichia coli transport lactose via...Ch. 2 - Prob. 10RQCh. 2 - List several functions of the outer membrane in...Ch. 2 - Prob. 12RQCh. 2 - Prob. 13RQCh. 2 - Prob. 14RQCh. 2 - Prob. 15RQCh. 2 - In a few sentences, indicate how the bacterial...Ch. 2 - Prob. 17RQCh. 2 - Prob. 18RQCh. 2 - Contrast the mechanism for motility in...Ch. 2 - Prob. 20RQCh. 2 - Prob. 21RQCh. 2 - List at least three features of eukaryotic cells...Ch. 2 - Prob. 23RQCh. 2 - Prob. 24RQCh. 2 - Prob. 25RQCh. 2 - Describe the major functions of the endoplasmic...Ch. 2 - Prob. 1AQCh. 2 - Prob. 2AQCh. 2 - Assume you are given two cultures, one of a...Ch. 2 - Prob. 4AQCh. 2 - Assume you are given two cultures of rod-shaped...
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- The sodium Na+/Glucose symport moves sodium and glucose across the plasma membrane. A) Describe was is meant by a symport. Include in your answer whether a symport is an example of a channel protein or a carrier protein. B) Why does the Na+/Glucose transporter work in only one direction? C) Describe how glucose can be both actively and passively transported into an out of the same cell. Include in your description how localization of transport proteins allows for these two different types of transport to occur in the same cell.arrow_forwardInhibition of the Na,K-ATPase might reasonably result in which of the following: a.) gradual cellular accumulation of K+ b.) gradual decrease in cytoplasmic Na+ c.) cell shrinkage d.) hyperpolarization of the membrane potential e.) reduced activity of many secondary active transportersarrow_forwardWhat is a secondary transporter? Why are they called secondary? What is [3 H]Leu? Explain both 3H and Leu.arrow_forward
- D-glucose is added to cells and the rate of glucose transport is plotted against D- glucose concentration. In the presence of the D-galactose derivative 6-0-benzyl-D- galactose, the curve is shifted to the right, indicating that D-glucose and the D- galactose derivative both compete for a place in the binding site. External factors such as the addition of ATP or ion gradients doesn't change the rate of absorption into cells. Which of the following is true, select all that apply: O the enzyme is a uniport O mediated transport is occurring O non-mediated transport occurring O passive transport is occurring O active transport is occurringarrow_forwardGlucose transport across cell membranes varies depending upon blood glucose levels. When glucose levels are high, glucose transport is accomplished via membrane transporters. When glucose concentrations are low, the transport of glucose across the membrane is dependent upon the sodium ion concentration. What types of transport is observed for glucose? A)simple diffusion at high [glucose], secondary active transport at low [glucose] B)facilitated diffusion at high [glucose], secondary active transport at low [glucose] C)simple diffusion at high [glucose], primary active transport at low [glucose] D)facilitated diffusion at high [glucose], primary active transport at low [glucose]arrow_forwardIs the movement of sodium through SGlut-1 during co-transport simple diffusion, facilitated diffusion, active transport, or secondary active transport? And is the movement of glucose through SGlut-1 during co-transport simple diffusion, facilitated diffusion, primary active transport, or secondary active transport?arrow_forward
- Define: simple diffusion, facilitated diffusion, primary active transport, secondary active transport and cotransport (by sure to address symport and antiport) . please explain this iin one sentense for each one please. note: I do not want more than one sentensearrow_forwardThe studies of Palade and colleagues used pulse chase labeling with radioactively labeled amino acids and auto radiography to visualize the location of newly synthesized proteins in pancreatic acinar cells. These early experiments provided invaluable information on protein synthesis and intercompartmental transport. New methods have replaced these early approaches, but two basic requirements are still necessary for any assay to study this type of protein transport. What are they, and how do recent experimental approaches meet these criteria?arrow_forwardWhat are three types of energy that can power an active transport pump? Provide an example of each type (eg Na/K pump)arrow_forward
- Active transport pumps are used to move sodium ions across the membranes of gill cells in freshwater fish species. Which of the following statements about the pumps is accurate?Explain. a) There are specific binding sites for sodium ions on the pump b) They require osmosis to carry the sodium ions into the cell c) This is an example of passive diffusion and ATP is required d) Their lipid based structure allows it to embed in the cell membranearrow_forwardProtein structure and function: a) Name two common post-translational modifications of proteins in the cell that will affect their structure/function. b) What are prions? Briefly describe their structure and function. C) Explain the principles of protein folding and significance of urea and Bmercaptoethanol in the experimental procedures addressing this question.arrow_forwardThe moving van analogy was used to explain what type of transport in the cell? Describe the different forms of transports related to the moving van analogy.arrow_forward
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