Brock Biology of Microorganisms (14th Edition)
14th Edition
ISBN: 9780321897398
Author: Michael T. Madigan, John M. Martinko, Kelly S. Bender, Daniel H. Buckley, David A. Stahl, Thomas Brock
Publisher: PEARSON
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Chapter 2, Problem 9RQ
Cells of Escherichia coli transport lactose via lac permease, glucose via the phosphotransferase system, and maltose via an ABC-type transporter. For each of these sugars describe: (1) the components of the transport system and (2) the source of energy that drives the transport event.
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A bacterial lactose transporter, which is highly specific for lactose, contains a Cys residue that is essential to its transport activity. Covalent reaction of N-ethylmaleimide (NEM) with this Cys residue irreversibly inactivates the transporter. A high concentration of lactose in the medium prevents inactivation by NEM, presumably by sterically protecting the Cys residue, which is in or near the lactose-binding site. You know nothing else about the transporter protein. Suggest an experiment that might allow you to determine the Mr of this Cys-containing transporter polypeptide.
According to the “Hawaiian Punch” principle, different compartments of the secretory and endocytic pathways are topologically equivalent to each other and to the extracellular space.
Explain how vesicular transport generates this topological equivalence.
These compartments are topologically equivalent because molecules can move between them without having to move out of the cell. Transport vesicles cause this “topological equivalence” because they are responsible for the actual movement of the molecules mentioned above. They break off from one compartment, carrying the necessary molecules, and then fuse to its targeted compartment; thus, making the compartments topologically equivalent.
During mitosis in mammalian cells, the nuclear envelope breaks down into vesicles. Are the topological properties of the cell altered by this event? Justify your answer.
Name the three classes of membrane transport proteins. Explain which one or ones of these classes is able to move glucose and which can move bicarbonate (HCO3−) against an electrochemical gradient. In the case of bicarbonate, but not glucose, the ΔG of the transport process has two terms. What are these two terms, and why does the second not apply to glucose? Why are cotransporters often referred to as examples of secondary active transport?
Chapter 2 Solutions
Brock Biology of Microorganisms (14th Edition)
Ch. 2.1 - Define the terms magnification and resolution.Ch. 2.1 - Prob. 2MQCh. 2.2 - Prob. 1MQCh. 2.2 - Prob. 2MQCh. 2.2 - How can cells be made to fluoresce?Ch. 2.3 - Prob. 1MQCh. 2.3 - Prob. 2MQCh. 2.4 - Prob. 1MQCh. 2.4 - Prob. 2MQCh. 2.5 - How do cocci and rods differ in morphology?
Ch. 2.5 - Prob. 2MQCh. 2.6 - What physical property of cells increases as cells...Ch. 2.6 - How can the small size and haploid genome of...Ch. 2.6 - What are the approximate limits to how small a...Ch. 2.7 - Prob. 1MQCh. 2.7 - Prob. 2MQCh. 2.8 - Prob. 1MQCh. 2.8 - Prob. 2MQCh. 2.9 - Compare and contrast simple transporters, the...Ch. 2.9 - Prob. 2MQCh. 2.10 - Why do bacterial cells need cell walls? Do all...Ch. 2.10 - Prob. 2MQCh. 2.10 - Prob. 3MQCh. 2.11 - Prob. 1MQCh. 2.11 - Prob. 2MQCh. 2.11 - Prob. 3MQCh. 2.11 - Prob. 4MQCh. 2.12 - Prob. 1MQCh. 2.12 - Prob. 2MQCh. 2.12 - Prob. 3MQCh. 2.13 - Prob. 1MQCh. 2.13 - Prob. 2MQCh. 2.14 - Prob. 1MQCh. 2.14 - Chapter Review Why would it be impossible for...Ch. 2.14 - Chapter Review How are magnetosomes and the...Ch. 2.15 - Prob. 1MQCh. 2.15 - Prob. 2MQCh. 2.16 - Prob. 1MQCh. 2.16 - Prob. 2MQCh. 2.16 - Prob. 3MQCh. 2.17 - Prob. 1MQCh. 2.17 - Prob. 2MQCh. 2.18 - Prob. 1MQCh. 2.18 - Prob. 2MQCh. 2.19 - Prob. 1MQCh. 2.19 - Prob. 2MQCh. 2.19 - Prob. 3MQCh. 2.19 - Chapter Review How does scotophobotaxis differ...Ch. 2.20 - Prob. 1MQCh. 2.20 - Prob. 2MQCh. 2.20 - Prob. 3MQCh. 2.21 - Prob. 1MQCh. 2.21 - Prob. 2MQCh. 2.21 - Prob. 3MQCh. 2.22 - Prob. 1MQCh. 2.22 - Prob. 2MQCh. 2.22 - Prob. 3MQCh. 2 - Prob. 1RQCh. 2 - Prob. 2RQCh. 2 - Prob. 3RQCh. 2 - What are the major morphologies of prokaryotic...Ch. 2 - How large can a bacterium be? How small? Why is it...Ch. 2 - Prob. 6RQCh. 2 - Prob. 7RQCh. 2 - Prob. 8RQCh. 2 - Cells of Escherichia coli transport lactose via...Ch. 2 - Prob. 10RQCh. 2 - List several functions of the outer membrane in...Ch. 2 - Prob. 12RQCh. 2 - Prob. 13RQCh. 2 - Prob. 14RQCh. 2 - Prob. 15RQCh. 2 - In a few sentences, indicate how the bacterial...Ch. 2 - Prob. 17RQCh. 2 - Prob. 18RQCh. 2 - Contrast the mechanism for motility in...Ch. 2 - Prob. 20RQCh. 2 - Prob. 21RQCh. 2 - List at least three features of eukaryotic cells...Ch. 2 - Prob. 23RQCh. 2 - Prob. 24RQCh. 2 - Prob. 25RQCh. 2 - Describe the major functions of the endoplasmic...Ch. 2 - Prob. 1AQCh. 2 - Prob. 2AQCh. 2 - Assume you are given two cultures, one of a...Ch. 2 - Prob. 4AQCh. 2 - Assume you are given two cultures of rod-shaped...
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- Name the three classes of membrane transport proteins. Explain which one or ones of these classes is able to move glucose and which can move bicarbonate (HCO3 −) against an electrochemical gradient. In the case of bicarbonate, but not glucose, the ΔG of the transport process has two terms.What are these two terms, and why does the second not apply to glucose? Why are cotransporters often referred to as examples of secondary active transport?arrow_forwardWhile endocytosis and exocytosis refer to the direction in which a vesicle moves into and out of a cell respectively, it is important to think about what is being moved with and within the vesicles. To demonstrate how this process is utilized in human cells we will focus on the human body's insulin response. The purpose of the insulin response is to create a pathway for glucose entry into cells by releasing glucose transporters into the membrane. Cell Type #1- insulin producers: In humans, pancreatic B-cells create steroid insulin that can be packaged into vesicles starting at the ER. These vesicles are prepared for exocytosis so that the insulin can be released from the pancreatic B - cells and get into the bloodstream when signaled to do so. When glucose is present in the blood stream these cells will release the pre-packed insulin into the blood stream. Cell Type #2- insulin responders: Other cells do not produce insulin but instead respond when they have received the insulin signal…arrow_forwardThe sodium Na+/Glucose symport moves sodium and glucose across the plasma membrane. A) Describe was is meant by a symport. Include in your answer whether a symport is an example of a channel protein or a carrier protein. B) Why does the Na+/Glucose transporter work in only one direction? C) Describe how glucose can be both actively and passively transported into an out of the same cell. Include in your description how localization of transport proteins allows for these two different types of transport to occur in the same cell.arrow_forward
- Ouabain is a specific inhibitor of the active transport of sodium ions out of the cell and is therefore a valuable tool in studies of membrane transport mechanisms. Which of the following processes in your own body would you expect to be sensitive to inhibition by ouabain? Explain your answer in each case. a) Facilitated diffusion of glucose into a muscle cell b) Active transport of dietary phenylalanine across the intestinal mucosa c) Uptake of potassium ions by red blood cells d) Active uptake of lactose by the bacteria in your intestinearrow_forwardWhat is the difference of transport processes for glucose in bacteria and eukaryotes? What are the pathways and enzymes involved?arrow_forwardWhat is a secondary transporter? Why are they called secondary? What is [3 H]Leu? Explain both 3H and Leu.arrow_forward
- Endocytosis and exocytosis are both forms of [ ACTIVE / PASSIVE ] transport that [ DO / DO NOT ] require energy. Outline an example/scenario in which a cell would need to perform a form of endocytosis. Outline an example/scenario in which a cell would need to perform exocytosis. a) Name the 3 specific types of endocytosis. b) Exocytosis is considered the opposite/reverse process as? Pls helparrow_forwardWrite two examples of glycosaminoglycans, and explain the roles that are not dependent on anchorage in the plasma membrane.arrow_forwardTwo strains of Steptococcus mutans, A and B, use sucrose (table sugar) as a carbon source. The first step in the process of sucrose utilization is the passage of sucrose through a sucrose transporter protein in the membrane. The characteristics of the two transport proteins are as follows: Strain B Km 1000 mM 10 mM Vmax 1000 mmol min 100 mmol min a) Assuming that the rate of sucrose uptake is the rate limiting step in growth, which strain will grow faster if the concentration of sucrose is: 10 mM? 100 mM? 1000 mM? b) One strain was isolated from the soil and the other from the floor at Toscanini's Ice Cream, which was likely to be which? Why?arrow_forward
- Define: simple diffusion, facilitated diffusion, primary active transport, secondary active transport and cotransport (by sure to address symport and antiport) . please explain this iin one sentense for each one please. note: I do not want more than one sentensearrow_forwardDescribe the symport process by which cells lining the small intestine import glucose. What ion is responsible for the transport, and what two particular features facilitate the energetically favored movement of this ion across the plasma membrane?arrow_forwardA 65-year-old man with severe atherosclerotic coronary artery disease comes to the emergency department because of a 12-hour history of chest pain. Plasma activity of the MB isozyme of creatine kinase (MB-CK) is markedly increased. Which of the following processes is the most likely explanation for the increased plasma MB-CK? (A) Cell membrane damage (B) Endoplasmic reticulum dilation (C) Mitochondrial swelling (D) Polysome dissociation (E) Sodium pump dysfunctionarrow_forward
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