Brock Biology of Microorganisms (14th Edition)
Brock Biology of Microorganisms (14th Edition)
14th Edition
ISBN: 9780321897398
Author: Michael T. Madigan, John M. Martinko, Kelly S. Bender, Daniel H. Buckley, David A. Stahl, Thomas Brock
Publisher: PEARSON
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Chapter 2.13, Problem 1MQ
Summary Introduction

Cell wall and cytoplasmic membrane are considered as the most vital structures of a cell. In addition to these structures, bacterial and archaeal cells also possess several other structures or layers that are in contact with the external environment.  

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A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?   The cell membranes are more resistant than normal.   An isotonic solution had been added instead of water.   A solution of 0.1 M NaCl had been added instead of water.   Not enough water had been added to the red blood cell pellet.   The man had sickle-cell anaemia.
A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?   The cell membranes are more resistant than normal.   An isotonic solution had been added instead of water.   A solution of 0.1 M NaCl had been added instead of water.   Not enough water had been added to the red blood cell pellet.   The man had sickle-cell anaemia.
With reference to their absorption spectra of the oxy haemoglobin intact line) and deoxyhemoglobin (broken line) shown in Figure 2 below, how would you best explain the reason why there are differences in the major peaks of the spectra? Figure 2. SPECTRA OF OXYGENATED AND DEOXYGENATED HAEMOGLOBIN OBTAINED WITH THE RECORDING SPECTROPHOTOMETER 1.4 Abs < 0.8 06 0.4 400 420 440 460 480 500 520 540 560 580 600 nm 1. The difference in the spectra is due to a pH change in the deoxy-haemoglobin due to uptake of CO2- 2. There is more oxygen-carrying plasma in the oxy-haemoglobin sample. 3. The change in Mr due to oxygen binding causes the oxy haemoglobin to have a higher absorbance peak. 4. Oxy-haemoglobin is contaminated by carbaminohemoglobin, and therefore has a higher absorbance peak 5. Oxy-haemoglobin absorbs more light of blue wavelengths and less of red wavelengths than deoxy-haemoglobin

Chapter 2 Solutions

Brock Biology of Microorganisms (14th Edition)

Ch. 2.5 - Prob. 2MQCh. 2.6 - What physical property of cells increases as cells...Ch. 2.6 - How can the small size and haploid genome of...Ch. 2.6 - What are the approximate limits to how small a...Ch. 2.7 - Prob. 1MQCh. 2.7 - Prob. 2MQCh. 2.8 - Prob. 1MQCh. 2.8 - Prob. 2MQCh. 2.9 - Compare and contrast simple transporters, the...Ch. 2.9 - Prob. 2MQCh. 2.10 - Why do bacterial cells need cell walls? Do all...Ch. 2.10 - Prob. 2MQCh. 2.10 - Prob. 3MQCh. 2.11 - Prob. 1MQCh. 2.11 - Prob. 2MQCh. 2.11 - Prob. 3MQCh. 2.11 - Prob. 4MQCh. 2.12 - Prob. 1MQCh. 2.12 - Prob. 2MQCh. 2.12 - Prob. 3MQCh. 2.13 - Prob. 1MQCh. 2.13 - Prob. 2MQCh. 2.14 - Prob. 1MQCh. 2.14 - Chapter Review Why would it be impossible for...Ch. 2.14 - Chapter Review How are magnetosomes and the...Ch. 2.15 - Prob. 1MQCh. 2.15 - Prob. 2MQCh. 2.16 - Prob. 1MQCh. 2.16 - Prob. 2MQCh. 2.16 - Prob. 3MQCh. 2.17 - Prob. 1MQCh. 2.17 - Prob. 2MQCh. 2.18 - Prob. 1MQCh. 2.18 - Prob. 2MQCh. 2.19 - Prob. 1MQCh. 2.19 - Prob. 2MQCh. 2.19 - Prob. 3MQCh. 2.19 - Chapter Review How does scotophobotaxis differ...Ch. 2.20 - Prob. 1MQCh. 2.20 - Prob. 2MQCh. 2.20 - Prob. 3MQCh. 2.21 - Prob. 1MQCh. 2.21 - Prob. 2MQCh. 2.21 - Prob. 3MQCh. 2.22 - Prob. 1MQCh. 2.22 - Prob. 2MQCh. 2.22 - Prob. 3MQCh. 2 - Prob. 1RQCh. 2 - Prob. 2RQCh. 2 - Prob. 3RQCh. 2 - What are the major morphologies of prokaryotic...Ch. 2 - How large can a bacterium be? How small? Why is it...Ch. 2 - Prob. 6RQCh. 2 - Prob. 7RQCh. 2 - Prob. 8RQCh. 2 - Cells of Escherichia coli transport lactose via...Ch. 2 - Prob. 10RQCh. 2 - List several functions of the outer membrane in...Ch. 2 - Prob. 12RQCh. 2 - Prob. 13RQCh. 2 - Prob. 14RQCh. 2 - Prob. 15RQCh. 2 - In a few sentences, indicate how the bacterial...Ch. 2 - Prob. 17RQCh. 2 - Prob. 18RQCh. 2 - Contrast the mechanism for motility in...Ch. 2 - Prob. 20RQCh. 2 - Prob. 21RQCh. 2 - List at least three features of eukaryotic cells...Ch. 2 - Prob. 23RQCh. 2 - Prob. 24RQCh. 2 - Prob. 25RQCh. 2 - Describe the major functions of the endoplasmic...Ch. 2 - Prob. 1AQCh. 2 - Prob. 2AQCh. 2 - Assume you are given two cultures, one of a...Ch. 2 - Prob. 4AQCh. 2 - Assume you are given two cultures of rod-shaped...
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