Brock Biology of Microorganisms (14th Edition)
14th Edition
ISBN: 9780321897398
Author: Michael T. Madigan, John M. Martinko, Kelly S. Bender, Daniel H. Buckley, David A. Stahl, Thomas Brock
Publisher: PEARSON
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Chapter 2.4, Problem 1MQ
Summary Introduction
In 1931, the first electron microscope was developed by the physicist Ernst Ruska and the electrical engineer Max Knoll. The electron microscope uses electron beam as a source of illumination and
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aaBb: ?
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A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?
The cell membranes are more resistant than normal.
An isotonic solution had been added instead of water.
A solution of 0.1 M NaCl had been added instead of water.
Not enough water had been added to the red blood cell pellet.
The man had sickle-cell anaemia.
Chapter 2 Solutions
Brock Biology of Microorganisms (14th Edition)
Ch. 2.1 - Define the terms magnification and resolution.Ch. 2.1 - Prob. 2MQCh. 2.2 - Prob. 1MQCh. 2.2 - Prob. 2MQCh. 2.2 - How can cells be made to fluoresce?Ch. 2.3 - Prob. 1MQCh. 2.3 - Prob. 2MQCh. 2.4 - Prob. 1MQCh. 2.4 - Prob. 2MQCh. 2.5 - How do cocci and rods differ in morphology?
Ch. 2.5 - Prob. 2MQCh. 2.6 - What physical property of cells increases as cells...Ch. 2.6 - How can the small size and haploid genome of...Ch. 2.6 - What are the approximate limits to how small a...Ch. 2.7 - Prob. 1MQCh. 2.7 - Prob. 2MQCh. 2.8 - Prob. 1MQCh. 2.8 - Prob. 2MQCh. 2.9 - Compare and contrast simple transporters, the...Ch. 2.9 - Prob. 2MQCh. 2.10 - Why do bacterial cells need cell walls? Do all...Ch. 2.10 - Prob. 2MQCh. 2.10 - Prob. 3MQCh. 2.11 - Prob. 1MQCh. 2.11 - Prob. 2MQCh. 2.11 - Prob. 3MQCh. 2.11 - Prob. 4MQCh. 2.12 - Prob. 1MQCh. 2.12 - Prob. 2MQCh. 2.12 - Prob. 3MQCh. 2.13 - Prob. 1MQCh. 2.13 - Prob. 2MQCh. 2.14 - Prob. 1MQCh. 2.14 - Chapter Review Why would it be impossible for...Ch. 2.14 - Chapter Review How are magnetosomes and the...Ch. 2.15 - Prob. 1MQCh. 2.15 - Prob. 2MQCh. 2.16 - Prob. 1MQCh. 2.16 - Prob. 2MQCh. 2.16 - Prob. 3MQCh. 2.17 - Prob. 1MQCh. 2.17 - Prob. 2MQCh. 2.18 - Prob. 1MQCh. 2.18 - Prob. 2MQCh. 2.19 - Prob. 1MQCh. 2.19 - Prob. 2MQCh. 2.19 - Prob. 3MQCh. 2.19 - Chapter Review How does scotophobotaxis differ...Ch. 2.20 - Prob. 1MQCh. 2.20 - Prob. 2MQCh. 2.20 - Prob. 3MQCh. 2.21 - Prob. 1MQCh. 2.21 - Prob. 2MQCh. 2.21 - Prob. 3MQCh. 2.22 - Prob. 1MQCh. 2.22 - Prob. 2MQCh. 2.22 - Prob. 3MQCh. 2 - Prob. 1RQCh. 2 - Prob. 2RQCh. 2 - Prob. 3RQCh. 2 - What are the major morphologies of prokaryotic...Ch. 2 - How large can a bacterium be? How small? Why is it...Ch. 2 - Prob. 6RQCh. 2 - Prob. 7RQCh. 2 - Prob. 8RQCh. 2 - Cells of Escherichia coli transport lactose via...Ch. 2 - Prob. 10RQCh. 2 - List several functions of the outer membrane in...Ch. 2 - Prob. 12RQCh. 2 - Prob. 13RQCh. 2 - Prob. 14RQCh. 2 - Prob. 15RQCh. 2 - In a few sentences, indicate how the bacterial...Ch. 2 - Prob. 17RQCh. 2 - Prob. 18RQCh. 2 - Contrast the mechanism for motility in...Ch. 2 - Prob. 20RQCh. 2 - Prob. 21RQCh. 2 - List at least three features of eukaryotic cells...Ch. 2 - Prob. 23RQCh. 2 - Prob. 24RQCh. 2 - Prob. 25RQCh. 2 - Describe the major functions of the endoplasmic...Ch. 2 - Prob. 1AQCh. 2 - Prob. 2AQCh. 2 - Assume you are given two cultures, one of a...Ch. 2 - Prob. 4AQCh. 2 - Assume you are given two cultures of rod-shaped...
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- A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation? The cell membranes are more resistant than normal. An isotonic solution had been added instead of water. A solution of 0.1 M NaCl had been added instead of water. Not enough water had been added to the red blood cell pellet. The man had sickle-cell anaemia.arrow_forwardWith reference to their absorption spectra of the oxy haemoglobin intact line) and deoxyhemoglobin (broken line) shown in Figure 2 below, how would you best explain the reason why there are differences in the major peaks of the spectra? Figure 2. SPECTRA OF OXYGENATED AND DEOXYGENATED HAEMOGLOBIN OBTAINED WITH THE RECORDING SPECTROPHOTOMETER 1.4 Abs < 0.8 06 0.4 400 420 440 460 480 500 520 540 560 580 600 nm 1. The difference in the spectra is due to a pH change in the deoxy-haemoglobin due to uptake of CO2- 2. There is more oxygen-carrying plasma in the oxy-haemoglobin sample. 3. The change in Mr due to oxygen binding causes the oxy haemoglobin to have a higher absorbance peak. 4. Oxy-haemoglobin is contaminated by carbaminohemoglobin, and therefore has a higher absorbance peak 5. Oxy-haemoglobin absorbs more light of blue wavelengths and less of red wavelengths than deoxy-haemoglobinarrow_forwardWith reference to their absorption spectra of the oxy haemoglobin intact line) and deoxyhemoglobin (broken line) shown in Figure 2 below, how would you best explain the reason why there are differences in the major peaks of the spectra? Figure 2. SPECTRA OF OXYGENATED AND DEOXYGENATED HAEMOGLOBIN OBTAINED WITH THE RECORDING SPECTROPHOTOMETER 1.4 Abs < 0.8 06 0.4 400 420 440 460 480 500 520 540 560 580 600 nm 1. The difference in the spectra is due to a pH change in the deoxy-haemoglobin due to uptake of CO2- 2. There is more oxygen-carrying plasma in the oxy-haemoglobin sample. 3. The change in Mr due to oxygen binding causes the oxy haemoglobin to have a higher absorbance peak. 4. Oxy-haemoglobin is contaminated by carbaminohemoglobin, and therefore has a higher absorbance peak 5. Oxy-haemoglobin absorbs more light of blue wavelengths and less of red wavelengths than deoxy-haemoglobinarrow_forward
- Which ONE of the following is FALSE regarding haemoglobin? It has two alpha subunits and two beta subunits. The subunits are joined by disulphide bonds. Each subunit covalently binds a haem group. Conformational change in one subunit can be transmitted to another. There are many variant ("mutant") forms of haemoglobin that are not harmful.arrow_forwardWhich ONE of the following is FALSE regarding haemoglobin? It has two alpha subunits and two beta subunits. The subunits are joined by disulphide bonds. Each subunit covalently binds a haem group. Conformational change in one subunit can be transmitted to another. There are many variant ("mutant") forms of haemoglobin that are not harmful.arrow_forwardDuring a routine medical check up of a healthy man it was found that his haematocrit value was highly unusual – value of 60%. What one of the options below is the most likely reason? He will have a diet high in iron. He is likely to be suffering from anaemia. He lives at high altitude. He has recently recovered from an accident where he lost a lot of blood. He has a very large body size.arrow_forward
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