Biochemistry: The Molecular Basis of Life
6th Edition
ISBN: 9780190209896
Author: Trudy McKee, James R. McKee
Publisher: Oxford University Press
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Question
Chapter 2, Problem 9RQ
Summary Introduction
To review:
Definition of the following terminologies:
(a) Glycocalyx
(b) Extracellular matrix
(c) Cell cortex
(d) Endoplasmic reticulum (ER)
(e) ER lumen
Introduction:
The eukaryotic cell is more sophisticated as compared to the prokaryotic cell. The reason for this sophistication is the tight and controlled regulation of various cellular processes that take place in the eukaryotes. Also, these cells have membrane-bound organelles, which are absent in case of prokaryotes.
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Fura-2 Fluorescence (Arbitrary Unit)
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[Ca2+]=2970nM, 25°C
[Ca2+] 2970nM, 4°C
[Ca2+]=0.9nM, 25°C
[Ca2+] = 0.9nM, 4°C
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Wavelength (nm)
←
<
The figure on the LHS shows the excitation spectra of Fura-2 (Em
= 510 nm) in 2 solutions with two different Ca2+ ion concentration
as indicated. Except for temperature, the setting for excitation &
signal acquisition was identical.<
ப
a) The unit in Y-axis is arbitrary (unspecified). Why? <
<
b) Compare & contrast the excitation wavelength of the Isosbestic
Point of Fura-2 at 25 °C & 4 °C. Give a possible reason for the
discrepancy. <
c) The fluorescence intensity at 25 °C & 4 °C are different. Explain
why with the concept of electronic configuration. <
Chapter 2 Solutions
Biochemistry: The Molecular Basis of Life
Ch. 2 - Prob. 1RQCh. 2 - Prob. 2RQCh. 2 - Prob. 3RQCh. 2 - Prob. 4RQCh. 2 - Prob. 5RQCh. 2 - Prob. 6RQCh. 2 - Prob. 7RQCh. 2 - Prob. 8RQCh. 2 - Prob. 9RQCh. 2 - Prob. 10RQ
Ch. 2 - Prob. 11RQCh. 2 - Prob. 12RQCh. 2 - Prob. 13RQCh. 2 - Prob. 14RQCh. 2 - Prob. 15RQCh. 2 - Prob. 16RQCh. 2 - Prob. 17RQCh. 2 - Prob. 18RQCh. 2 - Prob. 19RQCh. 2 - Prob. 20RQCh. 2 - Prob. 21RQCh. 2 - Prob. 22RQCh. 2 - Prob. 23RQCh. 2 - Prob. 24RQCh. 2 - Prob. 25RQCh. 2 - Prob. 26RQCh. 2 - Prob. 27RQCh. 2 - Prob. 28RQCh. 2 - Prob. 29RQCh. 2 - Prob. 30RQCh. 2 - Prob. 31RQCh. 2 - Prob. 32RQCh. 2 - Prob. 33RQCh. 2 - Prob. 34RQCh. 2 - Prob. 35RQCh. 2 - Prob. 36RQCh. 2 - Prob. 37RQCh. 2 - Prob. 38RQCh. 2 - Prob. 39RQCh. 2 - Prob. 40RQCh. 2 - Prob. 41RQCh. 2 - Prob. 42RQCh. 2 - Prob. 43RQCh. 2 - Prob. 44RQCh. 2 - Prob. 45RQCh. 2 - Prob. 46RQCh. 2 - Prob. 47RQCh. 2 - Prob. 48RQCh. 2 - Prob. 49RQCh. 2 - Prob. 50RQCh. 2 - Prob. 51FBCh. 2 - Prob. 52FBCh. 2 - Prob. 53FBCh. 2 - Prob. 54FBCh. 2 - Prob. 55FBCh. 2 - Prob. 56FBCh. 2 - Prob. 57FBCh. 2 - Prob. 58FBCh. 2 - Prob. 59FBCh. 2 - Prob. 60FBCh. 2 - Prob. 61SACh. 2 - Prob. 62SACh. 2 - Prob. 63SACh. 2 - Prob. 64SACh. 2 - Prob. 65SACh. 2 - Prob. 66TQCh. 2 - Prob. 67TQCh. 2 - Prob. 68TQCh. 2 - Prob. 69TQCh. 2 - Prob. 70TQCh. 2 - Prob. 71TQCh. 2 - Prob. 72TQCh. 2 - Prob. 73TQCh. 2 - Prob. 1QCh. 2 - Prob. 2QCh. 2 - Prob. 3QCh. 2 - Prob. 4Q
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