The way by which a bacterium can be normal and beneficial, but also be pathogenic. Concept introduction: Propionibacterium is a gram-positive, small and rod-shaped diphtheroids commonly occurring on the skin. They can be trapped by hair follicles in the skin.

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Chapter 19, Problem 9CT

Summary Introduction

To determine:

The way by which a bacterium can be normal and beneficial, but also be pathogenic.

Concept introduction:

Propionibacterium is a gram-positive, small and rod-shaped diphtheroids commonly occurring on the skin. They can be trapped by hair follicles in the skin.

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Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…

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In oxidase test with Pseudomonas aeruginosa, the cell cultures on the slide turn colorless to be purple after tetra-methyl-p-phenylenediamine dihydrochloride (TMPD) is added. In the reaction, OTMPD is electron acceptor O cytochrome c is the electron source oxygen is terminal electron acceptor OH2 produced is electron donor

Answer 2

Question

Chapter 19, Problem 9CT

Summary Introduction

To determine:

The way by which a bacterium can be normal and beneficial, but also be pathogenic.

Concept introduction:

Propionibacterium is a gram-positive, small and rod-shaped diphtheroids commonly occurring on the skin. They can be trapped by hair follicles in the skin.

Expert Solution & Answer

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Answer 3

Question

Chapter 19, Problem 9CT

Summary Introduction

To determine:

The way by which a bacterium can be normal and beneficial, but also be pathogenic.

Concept introduction:

Propionibacterium is a gram-positive, small and rod-shaped diphtheroids commonly occurring on the skin. They can be trapped by hair follicles in the skin.

Expert Solution & Answer

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Answer 4

Question

Chapter 19, Problem 9CT

Summary Introduction

To determine:

The way by which a bacterium can be normal and beneficial, but also be pathogenic.

Concept introduction:

Propionibacterium is a gram-positive, small and rod-shaped diphtheroids commonly occurring on the skin. They can be trapped by hair follicles in the skin.

Expert Solution & Answer

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Answer 5

Chapter 19, Problem 9CT

Summary Introduction

To determine:

The way by which a bacterium can be normal and beneficial, but also be pathogenic.

Concept introduction:

Propionibacterium is a gram-positive, small and rod-shaped diphtheroids commonly occurring on the skin. They can be trapped by hair follicles in the skin.

Answer 6

Chapter 19, Problem 9CT

Summary Introduction

To determine:

The way by which a bacterium can be normal and beneficial, but also be pathogenic.

Concept introduction:

Propionibacterium is a gram-positive, small and rod-shaped diphtheroids commonly occurring on the skin. They can be trapped by hair follicles in the skin.

Answer 7

Chapter 19, Problem 9CT

Summary Introduction

To determine:

The way by which a bacterium can be normal and beneficial, but also be pathogenic.

Concept introduction:

Propionibacterium is a gram-positive, small and rod-shaped diphtheroids commonly occurring on the skin. They can be trapped by hair follicles in the skin.

Answer 8

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The way by which a bacterium can be normal and beneficial, but also be pathogenic. Concept introduction: Propionibacterium is a gram-positive, small and rod-shaped diphtheroids commonly occurring on the skin. They can be trapped by hair follicles in the skin.

Solution Summary: The author explains that Propionibacterium is a gram-positive, rod-shaped diphtheroids commonly occurring on the skin. They produce propionic acid, vitamin B12, tetrapyrote

Videos

To determine:

The way by which a bacterium can be normal and beneficial, but also be pathogenic.

Concept introduction:

Propionibacterium is a gram-positive, small and rod-shaped diphtheroids commonly occurring on the skin. They can be trapped by hair follicles in the skin.

Want to see the full answer?

Chapter 19 Solutions

The way by which a bacterium can be normal and beneficial, but also be pathogenic. Concept introduction: Propionibacterium is a gram-positive, small and rod-shaped diphtheroids commonly occurring on the skin. They can be trapped by hair follicles in the skin.

Solution Summary: The author explains that Propionibacterium is a gram-positive, rod-shaped diphtheroids commonly occurring on the skin. They produce propionic acid, vitamin B12, tetrapyrote

Videos

To determine: The way by which a bacterium can be normal and beneficial, but also be pathogenic. Concept introduction: Propionibacterium is a gram-positive, small and rod-shaped diphtheroids commonly occurring on the skin. They can be trapped by hair follicles in the skin.

Want to see the full answer?

Chapter 19 Solutions

To determine:

The way by which a bacterium can be normal and beneficial, but also be pathogenic.

Concept introduction:

Propionibacterium is a gram-positive, small and rod-shaped diphtheroids commonly occurring on the skin. They can be trapped by hair follicles in the skin.