Which other viruses should be eradicated as smallpox virus is eradicated in nature and also describe the features of their biology that make them suitable candidate. Introduction: Small pox is defined as an infectious viral disease caused by Variola major and Variola minor. It is a deadly disease which is highly communicable, as it spread from person to person.

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Chapter 19, Problem 7CT

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Which other viruses should be eradicated as smallpox virus is eradicated in nature and also describe the features of their biology that make them suitable candidate.

Introduction:

Small pox is defined as an infectious viral disease caused by Variola major and Variola minor. It is a deadly disease which is highly communicable, as it spread from person to person.

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Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…

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