EBK MICROBIOLOGY:W/DISEASES BY BODY...-
5th Edition
ISBN: 9780134608242
Author: BAUMAN
Publisher: PEARSON
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Chapter 19, Problem 2FIB
Summary Introduction
Introduction:
Staphylococcus is a genus of facultative anaerobic bacteria. Staphylococcus is the most common cause of pimples, folliculitis, and other associated functions. The cells are spherical in shape and are arranged in grape-like clusters. The bacteria of this genus are salt tolerant and hence are able to grow on the salty surface of the cutaneous membrane of humans.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 19 Solutions
EBK MICROBIOLOGY:W/DISEASES BY BODY...-
Ch. 19 - Prob. 1TMWCh. 19 - Prob. 2TMWCh. 19 - Buruli Ulcer Jacques liked living in the...Ch. 19 - A New Cause of Spots Fifty-two-year-old David has...Ch. 19 - Prob. 3TMWCh. 19 - A Painful Rash A mother brings her three-year-old...Ch. 19 - A Child with Warts Ten-year-old Rudy has several...Ch. 19 - Grandfathers Shingles The Davises were excited...Ch. 19 - Prob. 4TMWCh. 19 - Prob. 4CCS
Ch. 19 - Prob. 5TMWCh. 19 - Diagnosis in the Desert In the 1970s,...Ch. 19 - Prob. 6TMWCh. 19 - Prob. 3EDCSCh. 19 - The epidermis ____. a. has an intricate network of...Ch. 19 - Prob. 2MCCh. 19 - Prob. 3MCCh. 19 - Prob. 4MCCh. 19 - Prob. 5MCCh. 19 - Prob. 6MCCh. 19 - Prob. 7MCCh. 19 - Prob. 8MCCh. 19 - Prob. 9MCCh. 19 - Prob. 10MCCh. 19 - Prob. 11MCCh. 19 - Prob. 12MCCh. 19 - Prob. 13MCCh. 19 - Prob. 14MCCh. 19 - Prob. 15MCCh. 19 - Prob. 1MCh. 19 - Prob. 1FIBCh. 19 - Prob. 2FIBCh. 19 - Prob. 3FIBCh. 19 - Prob. 4FIBCh. 19 - Prob. 5FIBCh. 19 - Prob. 6FIBCh. 19 - Prob. 7FIBCh. 19 - Prob. 8FIBCh. 19 - Prob. 9FIBCh. 19 - Most pimples result from infection by ______ not...Ch. 19 - Prob. 1MTFCh. 19 - Prob. 2MTFCh. 19 - Prob. 3MTFCh. 19 - Prob. 4MTFCh. 19 - Prob. 5MTFCh. 19 - Prob. 1SACh. 19 - Prob. 2SACh. 19 - Considering that Pseudomonas aeruginosa and...Ch. 19 - Prob. 4SACh. 19 - Prob. 5SACh. 19 - Describe four enzymes produced by Staphylococcus,...Ch. 19 - Prob. 7SACh. 19 - Prob. 8SACh. 19 - Prob. 9SACh. 19 - Prob. 10SACh. 19 - Label each lesion seen in poxvirus infections.Ch. 19 - Prob. 2VICh. 19 - Prob. 3VICh. 19 - Prob. 1CTCh. 19 - Why is it more difficult to rid a community of a...Ch. 19 - Prob. 3CTCh. 19 - Prob. 4CTCh. 19 - Prob. 5CTCh. 19 - Most DNA viruses replicate within the nucleus of a...Ch. 19 - Prob. 7CTCh. 19 - Prob. 8CTCh. 19 - Prob. 9CTCh. 19 - Prob. 10CTCh. 19 - Prob. 11CTCh. 19 - Why do impetigo and erysipelas occur more commonly...Ch. 19 - Prob. 13CTCh. 19 - Prob. 14CTCh. 19 - Prob. 1CM
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