Biology
Biology
12th Edition
ISBN: 9781260494570
Author: Raven, Peter
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Chapter 18, Problem 2A
Summary Introduction

Introduction:

ENCODE stands for Encyclopedia of DNA Elements project is a collective effort to recognize all functional elements that are present in the genome of human. The primary interpretation of the work was 80% of sequence of DNA in the genome of human are functional.

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Which statement BEST differentiate metagenomics from proteomics? a The material used in metagenomics is mRNA while proteomic uses polypeptides. b The material used in metagenomics is DNA while proteomic uses polypeptides. c The material used in metagenomics is a transcriptome while proteomic uses polypeptides. d The material used in metagenomics is DNA from a mitochondrion while proteomic uses polypeptides.
Check all the statements that are TRUE regarding 16S or ITS (microbiome) illumina (NGS) sequencing vs. whole genome illumina (NGS) sequencing.    A. You don't need to trim the reads for 16S sequencing, but you do for whole genome sequencing.   B. Whole genome sequencing files will have more reads in them because genomes are bigger than 16S amplicons.   C. The read alignment and contig assembly steps would probably be harder for whole genome sequencing.   D. The 16S molecules being sequenced are always DNA, while for whole genome sequencing they could be RNA or DNA going into the illumina sequencer.   E. NGS for 16S and whole genome sequencing can both use sample indexes/barcodes to maximize efficiency.   F. Whole genome sequencing doesn't necessarily require you to know any of the sequences/targets before hand to design specific primers for.
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