Biology
12th Edition
ISBN: 9781260494570
Author: Raven, Peter
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Textbook Question
Chapter 18, Problem 1U
A genetic map provides
a. the sequence of the DNA in a genome.
b. the relative position of genes on chromosomes.
c. the location of sites of restriction enzyme cleavage in a known sequence of DNA.
d. the banding pattern of a chromosome.
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Which of the following does not occur during telomerase extension of parental
DNA?
a. Translocation of the telomerase across the parental DNA.
b. Extension of the DNA that is complementary to the telomerase RNA.
c. Polymerisation of the new daughter DNA strand by telomerase.
d. Binding of an RNA primer and synthesis of new DNA in a 5' to 3'
direction.
e. Binding of the telomerase to the telomere.
Which of the following is a function of a restriction endonuclease?
a.
It cleaves a DNA molecule at a specific site.
b.
It cleaves a DNA molecule at random locations.
c.
It removes specific codons from an mRNA molecule.
d.
It removes specific exons from an mRNA molecule.
Certain restriction endonucleases produce cohesive (sticky) ends. This means that they:
a.
stick tightly to the ends of the DNA they have cut.
b.
cut both DNA strands at the same base pair.
c.
make a staggered double-strand cut, leaving ends with a few nucleotides of single-stranded DNA protruding.
d.
cut in regions of high GC content, leaving ends that can form more hydrogen bonds than ends of high AT content.
e.
cut in regions of high AT content, leaving ends that can form more hydrogen bonds than ends of high GC content.
Chapter 18 Solutions
Biology
Ch. 18.1 - Prob. 1LOCh. 18.1 - Describe the pros and cons of restriction mapping,...Ch. 18.1 - Prob. 3LOCh. 18.2 - Discriminate between dideoxy terminator sequencing...Ch. 18.2 - Prob. 2LOCh. 18.3 - Describe the findings of the Human Genome Project.Ch. 18.3 - Prob. 2LOCh. 18.3 - Prob. 3LOCh. 18.4 - Prob. 1LOCh. 18.4 - Prob. 2LO
Ch. 18.4 - Prob. 3LOCh. 18.5 - Prob. 1LOCh. 18.5 - Prob. 2LOCh. 18.5 - Prob. 3LOCh. 18.6 - Prob. 1LOCh. 18 - Prob. 1DACh. 18 - If the human genome contains approximately 3...Ch. 18 - Prob. 1IQCh. 18 - Prob. 2IQCh. 18 - Prob. 3IQCh. 18 - Prob. 4IQCh. 18 - Prob. 5IQCh. 18 - Prob. 6IQCh. 18 - A genetic map provides a. the sequence of the DNA...Ch. 18 - Prob. 2UCh. 18 - Approximately how many genes are there in the...Ch. 18 - An open reading frame (ORF) is distinguished by...Ch. 18 - What is a BLAST search? a. A mechanism for...Ch. 18 - Prob. 6UCh. 18 - Prob. 7UCh. 18 - Prob. 8UCh. 18 - Prob. 1ACh. 18 - Prob. 2ACh. 18 - Prob. 3ACh. 18 - Prob. 4ACh. 18 - What information can be obtained from a DNA...Ch. 18 - Prob. 6ACh. 18 - Prob. 7ACh. 18 - You are in the early stages of a genome-sequencing...Ch. 18 - Genomic research can be used to determine if an...
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- The diagram illustrating the polymerase chain reaction (PCR) technique is provided below. How does the number of copies of the DNA region being amplified change at the end of each cycle of the polymerase chain reaction? Group of answer choices a. The number of copies triples (or triplicates). b. The number of copies does not change. c. The number of copies quadruples (or quadruplicates). d. The number of copies doubles (or duplicates). e. The number of copies halves.arrow_forwardWhat is the enzymatic function of restriction enzymes? Group of answer choices a. to cut nucleic acids at specific sites b. to join nucleotides during transcription c. to add new nucleotides to the growing strand of DNA d. to repair breaks in sugar - phosphate backbonesarrow_forwardDefine the following terms: a. DNA typing b. short tandem repeats c. DNA profile d. nucleosome e. retrotransposonarrow_forward
- In an EMSA, the binding of a protein to DNAa. prevents the DNA from being digested with a restrictionenzyme.b. causes the DNA to migrate more slowly through a gel.c. causes the DNA to migrate more quickly through a gel.d. inhibits the expression of any genes within the DNA.arrow_forwardPlace the steps of sanger sequencing in order.A. A fluorescent laser excites the fragments and records the wavelength consistent with a single nucleotide. B. ddNTPs bind and stop chain extension.C. DNA fragments are separated by size through a capillary tube. D. DNA polymerase copies the target region of template DNA.E. The final nucleotide of each fragment is labeled with a fluorescent tag.arrow_forwardWhich of the following molecules helps relieve the tension of unwinding parental DNA strands, by breaking DNA and rejoining it before it is replicated? a. Primase b. Single-stranded binding proteins c. Helicase d. Topoisomerasearrow_forward
- All of the following statements about telomerase are correct except: A. the RNA component acts as a template for the synthesis of a segment of DNA. B. it adds telomeric repeats to the 5'-ends of the DNA strands. C. it provides a mechanism for replicating the ends of linear chromosomes. D. it recognizes a G-rich single strand of DNA E. it is a reverse transcripcase.arrow_forwardWhat is a restriction endonuclease? Select one: a. It is an enzyme that cleaves at a specific nucleotide sequence. b. It restricts the movement of the DNA outside the nucleus. c. It proofreads the DNA for accidental damages and corrects any errors. d. It is an enzyme that separates the DNA double helix.arrow_forwardDefine the following terms:a. hypochromic effectb. DNA denaturationc. restriction endonucleasesd. DNA hybridizatione. Southern blottingarrow_forward
- A linear plasmid contains only two genes, which are transcribed in opposite directions, each one from the end, toward the center of the plasmid. Draw diagrams ofa. the plasmid DNA, showing the 5′ and 3′ ends of the nucleotide strands.b. the template strand for each gene.c. the positions of the transcription-initiation sites.d. the transcripts, showing the 5′ and 3′ ends.arrow_forwardFollowing a CRISPR mediated DNA double-strand break, how can the DNA be repaired by the cell? Choose all that apply. A. Base pairing between complementary sticky sides. B. Nonhomologous end joining. C. Homology directed repair. D. Crossing over between nonhomologous chromosomes.arrow_forwardArrange the following steps in the sequence they would happen in a DNA cloning experiment. a. sealing DNA fragments into vectors with DNA ligase; b. utilizing a probe to detect a clone in the library; c. sequencing the clone's DNA; d. creating a DNA library of clones; e. cutting genomic DNA with restriction enzymes. A. e,a,d,b,c B. a,d,b,c,e C. c,b,e,a,d D. e,d,a,c,barrow_forward
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