Biology
12th Edition
ISBN: 9781260494570
Author: Raven, Peter
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Chapter 18, Problem 6U
Summary Introduction
Introduction:
Shotgun sequencing is a method in genetics that is used for sequencing long strands of DNA. Genome assembly is simply the sequencing of genome produced after fragmentation of chromosome and then those fragments are sequenced and resulting sequences have been set back together.
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What is Sanger sequencing? Why do we use ddNTP? How to read a DNA sequence gel? c. What is a cDNA seq (RNA seq)? d. What is the main difference between a genomic and a transcriptome study?
Place the steps of sanger sequencing in order.A. A fluorescent laser excites the fragments and records the wavelength consistent with a single nucleotide.
B. ddNTPs bind and stop chain extension.C. DNA fragments are separated by size through a capillary tube.
D. DNA polymerase copies the target region of template DNA.E. The final nucleotide of each fragment is labeled with a fluorescent tag.
a. What type of nucleic acid and from what species would the scientist use to begin
construction of her genomic DNA library?
b. From what tissue would she isolate this nucleic acid?
c. What type of reagent would the scientist use to cut the genome into appropriately sized
fragments?
d. What size nucleic acid fragments would one aim to prepare for the library construction
so as to to avoid having to screen an overwhelming number of clones?
e. Into what vector would the scientist ligate her genomic DNA fragments?
f. What organism would the scientist use to propagate the clones of her genomic DNA
library?
g. From the information given in the problem determine what probe could be used to
screen the scientist's library to find her clone of interest ?
Chapter 18 Solutions
Biology
Ch. 18.1 - Prob. 1LOCh. 18.1 - Describe the pros and cons of restriction mapping,...Ch. 18.1 - Prob. 3LOCh. 18.2 - Discriminate between dideoxy terminator sequencing...Ch. 18.2 - Prob. 2LOCh. 18.3 - Describe the findings of the Human Genome Project.Ch. 18.3 - Prob. 2LOCh. 18.3 - Prob. 3LOCh. 18.4 - Prob. 1LOCh. 18.4 - Prob. 2LO
Ch. 18.4 - Prob. 3LOCh. 18.5 - Prob. 1LOCh. 18.5 - Prob. 2LOCh. 18.5 - Prob. 3LOCh. 18.6 - Prob. 1LOCh. 18 - Prob. 1DACh. 18 - If the human genome contains approximately 3...Ch. 18 - Prob. 1IQCh. 18 - Prob. 2IQCh. 18 - Prob. 3IQCh. 18 - Prob. 4IQCh. 18 - Prob. 5IQCh. 18 - Prob. 6IQCh. 18 - A genetic map provides a. the sequence of the DNA...Ch. 18 - Prob. 2UCh. 18 - Approximately how many genes are there in the...Ch. 18 - An open reading frame (ORF) is distinguished by...Ch. 18 - What is a BLAST search? a. A mechanism for...Ch. 18 - Prob. 6UCh. 18 - Prob. 7UCh. 18 - Prob. 8UCh. 18 - Prob. 1ACh. 18 - Prob. 2ACh. 18 - Prob. 3ACh. 18 - Prob. 4ACh. 18 - What information can be obtained from a DNA...Ch. 18 - Prob. 6ACh. 18 - Prob. 7ACh. 18 - You are in the early stages of a genome-sequencing...Ch. 18 - Genomic research can be used to determine if an...
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- Which of the following is FALSE about current Sanger dideoxy DNA sequencing procedures? a. Chain termination occurs during synthesis of a new DNA strand. b. Many steps can be automated. c. No DNA is synthesized in the procedure. d. Fluorescent molecules can be used to detect the DNA.arrow_forwardIn next-generation sequencing, which of these advances allows for massively parallel sequencing? a. Pieces of DNA are fixed to a surface, so we can tell which new nucleotides were added to each piece. b. DNA sequences are read in real-time as nucleotides are added to each piece. c. Each segment of the genome can be pieced back together through shotgun alignment d. Single molecules of DNA can be read without the need for amplification.arrow_forwardThe diagram illustrating the polymerase chain reaction (PCR) technique is provided below. How does the number of copies of the DNA region being amplified change at the end of each cycle of the polymerase chain reaction? Group of answer choices a. The number of copies triples (or triplicates). b. The number of copies does not change. c. The number of copies quadruples (or quadruplicates). d. The number of copies doubles (or duplicates). e. The number of copies halves.arrow_forward
- The CRISPR system may be used to manipulate the genome in human cells, because Cas9 can Select one: O a. methylate specific RNA sequence of target gene. O b. cleave double-stranded RNA sequence of target gene. O c. methylate double-stranded DNA sequence of target gene. O d. cleave double-stranded DNA sequence of target gene.arrow_forwardBy mistake, you add two primers to your DNA sequencing reaction. The primers anneal on the same strand but 20 bases apart. Which of the following best describes how your sequence will look?A. The sequence will be readable for the first 20 bases only.B. The detector will detect two nucleotides at about 75% of the positions.C. The sequence will be readable for the last 20 bases only.D. The detector will detect one nucleotide at about 75% of the positions.E. The detector will detect no nucleotides at any of the positions. Explain why it is B.arrow_forwardDescribe two of the applications for genome mapping.arrow_forward
- Q. How can you design your RT-PCR experiment to control for gDNA contamination? A. Use forward and reverse primers that bind to the same exon. B. Run a control lane where only RT was performed and not PCR. C. Run a control lane where mRNA has been amplified using PCR. D. Use forward and reverse primers that span the junction of 2 separate exons.arrow_forwardWhat is a restriction endonuclease? Select one: a. It is an enzyme that cleaves at a specific nucleotide sequence. b. It restricts the movement of the DNA outside the nucleus. c. It proofreads the DNA for accidental damages and corrects any errors. d. It is an enzyme that separates the DNA double helix.arrow_forwardWhich of the following best describes the process of DNA seqencing. a. DNA is seperated on a gel and the different bands are labled with flouroscent nucleotides and scanned with a laser. b. A laser is used to flurorescently label the nucleotides present with in the DNA , the DNA is run on a gel and then the DNA is droken into fragments c. Nucleotides are scanned with a laser and incrprorated into the DNA that has been seperated on a gel and then DNA is amplified with PCR. d. fragments of DNA are produced in a reaction that lables them with any of four different fluroscent dyes and the fragmented then are run on a gel and scanned with laser e. DNA is broken down into its constituents nucleotides and the nucleotides are then run on a gel and purified with a laserarrow_forward
- Which of the following is/are not required for DNAreplication to occur?a. DNA polymerase d. primersb. nucleotides e. helicasec. template DNA f. all are requiredarrow_forwardArrange the following steps in the sequence they would happen in a DNA cloning experiment. a. sealing DNA fragments into vectors with DNA ligase; b. utilizing a probe to detect a clone in the library; c. sequencing the clone's DNA; d. creating a DNA library of clones; e. cutting genomic DNA with restriction enzymes. A. e,a,d,b,c B. a,d,b,c,e C. c,b,e,a,d D. e,d,a,c,barrow_forwardCertain restriction endonucleases produce cohesive (sticky) ends. This means that they: a. stick tightly to the ends of the DNA they have cut. b. cut both DNA strands at the same base pair. c. make a staggered double-strand cut, leaving ends with a few nucleotides of single-stranded DNA protruding. d. cut in regions of high GC content, leaving ends that can form more hydrogen bonds than ends of high AT content. e. cut in regions of high AT content, leaving ends that can form more hydrogen bonds than ends of high GC content.arrow_forward
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