a.
To describe:
The epistasis relationship between sev- and yan- mutants.
Introduction:
It is given that the yan gene codes for a transcription factor. This transcription factor reduces R7 development in the five cells. These cells express Sevenless and are competent to become R7.
b.
To determine:
Whether the given proteins show an expected epistatic interaction.
Introduction:
Sevenless and Yan proteins both play a significant role in the signaling pathway. They function during
c.
To determine:
The method due to which two different null mutations in the R7 signaling pathway can evoke different mutant phenotypes.
Introduction:
When the phenotype of one gene depends on the presence of one or more modifier genes, this phenomenon is termed as epistasis. Null mutation makes a gene completely non-functional.
d.
To determine:
The reason due to which both Ras and Yan function downstream of Sevenless in the signaling pathway.
Introduction:
The epistasis of two genes that function in the same pathway relies on their order of action in the pathway. In the epistatic mutation, either the upstream gene products activate downstream gene products, or they reduce downstream gene products.
e.
To determine:
The mutant phenotype of the flies in the given question and whether yanD is expected to be epistatic to sev- or Rasg12V.
Introduction:
It is given that a researcher identifies flies with a constitutively active mutant allele of yan (yanD) that is dominant. It expresses yan even in cells when the Sevenless pathway is in active state.
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Genetics: From Genes to Genomes, 5th edition
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