Anatomy and Physiology: An Integrative Approach with Connect Access Card
3rd Edition
ISBN: 9781260254440
Author: Michael McKinley, Valerie O'Loughlin, Theresa Bidle
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 16.4, Problem 15WDL
What are the functions of the vitreous humor and the aqueous humor?
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 16 Solutions
Anatomy and Physiology: An Integrative Approach with Connect Access Card
Ch. 16.1 - LEARNING OBJECTIVE
1. Describe the general...Ch. 16.1 - How does a sensory receptor function as a...Ch. 16.1 - Prob. 2LOCh. 16.1 - Prob. 2WDLCh. 16.1 - Prob. 3LOCh. 16.1 - Prob. 4LOCh. 16.1 - Prob. 3WDLCh. 16.1 - Prob. 5LOCh. 16.1 - LEARNING OBJECTIVE
6. Classify the various types...Ch. 16.1 - Prob. 4WDL
Ch. 16.2 - Prob. 7LOCh. 16.2 - What are the three types of unencapsulated tactile...Ch. 16.2 - Prob. 8LOCh. 16.2 - Prob. 6WDLCh. 16.3 - Prob. 9LOCh. 16.3 - Prob. 10LOCh. 16.3 - What is the role of the mucus in detection of...Ch. 16.3 - Prob. 8WDLCh. 16.3 - Prob. 11LOCh. 16.3 - Prob. 12LOCh. 16.3 - Prob. 13LOCh. 16.3 - Prob. 1WDTCh. 16.3 - Prob. 9WDLCh. 16.3 - Prob. 10WDLCh. 16.4 - LEARNING OBJECTIVE
14. Describe the accessory...Ch. 16.4 - Prob. 11WDLCh. 16.4 - Prob. 12WDLCh. 16.4 - Prob. 15LOCh. 16.4 - What are the three eye tunics; what is the primary...Ch. 16.4 - Compare the anatomic structure of the cornea and...Ch. 16.4 - What are the functions of the vitreous humor and...Ch. 16.4 - Prob. 16LOCh. 16.4 - Prob. 17LOCh. 16.4 - Prob. 16WDLCh. 16.4 - Prob. 18LOCh. 16.4 - Prob. 19LOCh. 16.4 - LEARNING OBJECTIVE
20. Explain the bleaching...Ch. 16.4 - What are the differences between rods and cones...Ch. 16.4 - How does dark adaptation differ from light...Ch. 16.4 - What occurs during phototransduction of light?Ch. 16.4 - Prob. 21LOCh. 16.4 - Prob. 22LOCh. 16.4 - Prob. 2WDTCh. 16.4 - Prob. 20WDLCh. 16.4 - Prob. 21WDLCh. 16.5 - Prob. 23LOCh. 16.5 - Prob. 24LOCh. 16.5 - Prob. 25LOCh. 16.5 - Prob. 3WDTCh. 16.5 - Prob. 22WDLCh. 16.5 - Prob. 23WDLCh. 16.5 - Prob. 24WDLCh. 16.5 - Prob. 26LOCh. 16.5 - Prob. 27LOCh. 16.5 - Prob. 28LOCh. 16.5 - What are the steps for detecting sounds?Ch. 16.5 - Compare the difference in how we perceive pitch...Ch. 16.5 - Prob. 29LOCh. 16.5 - Prob. 27WDLCh. 16.5 - Prob. 30LOCh. 16.5 - Prob. 31LOCh. 16.5 - Prob. 32LOCh. 16.5 - Prob. 28WDLCh. 16.5 - Prob. 29WDLCh. 16 - Prob. 1DYBCh. 16 - Prob. 2DYBCh. 16 - Prob. 3DYBCh. 16 - Prob. 4DYBCh. 16 - Prob. 5DYBCh. 16 - Prob. 6DYBCh. 16 - _____ 7. Which ear structure is correctly matched...Ch. 16 - Prob. 8DYBCh. 16 - Prob. 9DYBCh. 16 - Prob. 10DYBCh. 16 - Prob. 11DYBCh. 16 - Prob. 12DYBCh. 16 - Prob. 13DYBCh. 16 - Prob. 14DYBCh. 16 - Prob. 15DYBCh. 16 - Prob. 16DYBCh. 16 - Prob. 17DYBCh. 16 - Prob. 18DYBCh. 16 - Describe the pathway by which sound waves enter...Ch. 16 - Explain how the vestibule and semicircular canals...Ch. 16 - Prob. 1CALCh. 16 - Prob. 2CALCh. 16 - Prob. 3CALCh. 16 - Prob. 4CALCh. 16 - Prob. 5CALCh. 16 - Prob. 1CSLCh. 16 - Prob. 2CSLCh. 16 - Prob. 3CSL
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