FUNDAMENTALS OF BIOCHEMISTRY-ACCESS
5th Edition
ISBN: 9781119498742
Author: Voet
Publisher: WILEY
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Chapter 16, Problem 32CQ
Summary Introduction
To explain: The reason behind two substrate-binding sites would promote efficient cellulose synthesis.
Concept introduction: The starch contains both amylose and amylopectin. Amylopectin is a branched
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The beta-lactamase hydrolyzes the lactam-ring in penicillin. Describe the mechanism
of hydrolysis, insuring to include the involvement of S, D, & K in the reaction sequence. Please help
To map the active site of beta-lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine.
Why doesn't D in this hexapeptide not participate in the hydrolysis of the beta-lactam ring even though S, K, and D are involved in the catalyst?
To map the active site of -lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine.
Using the experimental results described above derive the primary sequence of the active site hexapeptide. Please help!
Chapter 16 Solutions
FUNDAMENTALS OF BIOCHEMISTRY-ACCESS
Ch. 16 - Prob. 1ECh. 16 - Prob. 2ECh. 16 - 3. Phosphoglucokinase catalyzes the...Ch. 16 - Prob. 4ECh. 16 - 5. Individuals with McArdle’s disease often...Ch. 16 - Prob. 6ECh. 16 - Prob. 7ECh. 16 - 8. Subjects who consumed a meal containing a high...Ch. 16 - 9. Many diabetics do not respond to insulin...Ch. 16 - 10. Why does it make metabolic sense for the same...
Ch. 16 - Prob. 11ECh. 16 - 12. Some archaebacteria produce an enzyme with two...Ch. 16 - Prob. 13ECh. 16 - 14. Write the balanced equation for the catabolism...Ch. 16 - 15. Indicate the energy yield or cost, in ATP...Ch. 16 - 16. In the Cori cycle, the lactate product of...Ch. 16 - 17. Predict the effect of a...Ch. 16 - 18. Explain why an individual with a...Ch. 16 - Prob. 19ECh. 16 - Prob. 20ECh. 16 - 21. The cells that line the mammalian intestine...Ch. 16 - Prob. 22ECh. 16 - 23. One molecule of dietary glucose can be...Ch. 16 - 24. The free energy of hydrolysis of an ?(1?4)...Ch. 16 - 25. (a) What amino acid, other than alanine, can...Ch. 16 - Prob. 26CQCh. 16 - Prob. 27CQCh. 16 - 24. The free energy of hydrolysis of an ?(1 ?4)...Ch. 16 - 9. Would you expect the allosteric regulator AMP...Ch. 16 - 30. Explain why it makes metabolic sense for...Ch. 16 - 31. Fill in the blanks in the following diagram...Ch. 16 - Prob. 32CQCh. 16 - Prob. 33CQCh. 16 - Prob. 34CQCh. 16 - Prob. 1MTE
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- Which features of the curves in Figure 30-2 indicates that the enzyme is not consumed in the overall reaction? ES is lower in energy that E + S and EP is lower in energy than E + P. What does this tell you about the stability of ES versus E + S and EP versus E + P.arrow_forwardLooking at the figure 30-5 what intermolecular forces are present between the substrate and the enzyme and the substrate and cofactors.arrow_forwardprovide short answers to the followings Urgent!arrow_forward
- Pyruvate is accepted into the TCA cycle by a “feeder” reaction using the pyruvatedehydrogenase complex, resulting in acetyl-CoA and CO2. Provide a full mechanismfor this reaction utilizing the TPP cofactor. Include the roles of all cofactors.arrow_forwardB- Vitamins are converted readily into important metabolic cofactors. Deficiency inany one of them has serious side effects. a. The disease beriberi results from a vitamin B 1 (Thiamine) deficiency and ischaracterized by cardiac and neurological symptoms. One key diagnostic forthis disease is an increased level of pyruvate and α-ketoglutarate in thebloodstream. How does this vitamin deficiency lead to increased serumlevels of these factors? b. What would you expect the effect on the TCA intermediates for a patientsuffering from vitamin B 5 deficiency? c. What would you expect the effect on the TCA intermediates for a patientsuffering from vitamin B 2 /B 3 deficiency?arrow_forwardDraw the Krebs Cycle and show the entry points for the amino acids Alanine,Glutamic Acid, Asparagine, and Valine into the Krebs Cycle - (Draw the Mechanism). How many rounds of Krebs will be required to waste all Carbons of Glutamic Acidas CO2?arrow_forward
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