Study Guide for Campbell Biology
11th Edition
ISBN: 9780134443775
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Jane B. Reece, Martha R. Taylor, Michael A. Pollock
Publisher: PEARSON
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Textbook Question
Chapter 16, Problem 2TYK
Transformation involves
- a. the uptake of external genetic material.
- b. the creation of a strand of RNA from a DNA molecule.
- c. the infection of bacterial cells by phage.
- d. the type of semiconservative replication shown by DNA.
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Bacteria exposed to viruses incorporate sections of the virus’s DNA into the CRISPR array sequences in their genome. This mechanism allows bacteria to fight off the viruses, like an immune response: the information in CRISPR spacers served as “coordinates” for recognizing and cutting up invading DNA sequences. Describe what might happen under the conditions described after a bacteriophage infects a bacterial cell and releases its DNA into the bacterial cell.
Explain why:
1. The invading phage DNA is recognized by the Cas proteins but not inserted into the CRISPR array region of the bacterial genome: The bacteria will be unable to elicit an immune response and will succumb to the phase infection
2. The cas genes on the bacterial genome contains a missense mutation that increases its cleavage/cut activityThe bacteria will elicit an immune response that will successfully fight the phage infection
Plasmids are important for recombining DNA.
B.) What are the requirments of an expression vector.
A.) There is no change in the DNA sequence if nucleotides are added or removed, it will have no effect to the cell. B.) Mutations in the DNA sequence are all irreversible.
A. Statement A is correct
B. Statement B is correct
C. Both A and B are correct
D. Both A and B are incorrect
Chapter 16 Solutions
Study Guide for Campbell Biology
Ch. 16 - Hershey and Chase devised an experiment using...Ch. 16 - Review the structure of DNA by labeling the...Ch. 16 - Using different colors for heavy (parental) and...Ch. 16 - Look back to Interactive Question 16.2 and label...Ch. 16 - In this diagram of bacterial DNA replication,...Ch. 16 - Draw the last Okazaki fragment being formed on the...Ch. 16 - List the successive levels of packing in a...Ch. 16 - Prob. 1SYKCh. 16 - Prob. 2SYKCh. 16 - One of the reasons most scientists thought...
Ch. 16 - Transformation involves a. the uptake of external...Ch. 16 - Prob. 3TYKCh. 16 - Which of the following most closely represents...Ch. 16 - Prob. 5TYKCh. 16 - Prob. 6TYKCh. 16 - In their classic experiment, Meselson and Stahl a....Ch. 16 - The joining of nucleotides in the polymerization...Ch. 16 - DNA polymerase is not able to begin copying a DNA...Ch. 16 - Prob. 10TYKCh. 16 - Prob. 11TYKCh. 16 - Prob. 12TYKCh. 16 - Which of the following is least related to the...Ch. 16 - Prob. 14TYKCh. 16 - Prob. 15TYKCh. 16 - Prob. 16TYKCh. 16 - Prob. 17TYKCh. 16 - Which of the following statements about telomeres...Ch. 16 - You are trying to test your hypothesis that DNA...Ch. 16 - Given the experimental procedure explained in...Ch. 16 - Prob. 21TYKCh. 16 - Biologists have learned from the technique of...
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- Targeting topoisomerase or DNA gyrase with an inhibitory antibiotic would inhibit which of the following processes in the bacterial cell? A.Peptidoglycan synthesis B.Plasma membrane synthesis C.Translation D.DNA replicationarrow_forwardWhich of the following is defined as transformation? A. Transfer of genetic information via a plasmid B. Virus infects a host and deposits genetic material C. Free floating DNA is uptaken D. Transfer of genetic information from one bacterium to another 5.arrow_forwardIn generalized transduction.... Choose from the answers below: random fragments of degraded bacterial DNA are packaged in phage particles. the maximum size of transduced DNA fragments is dependent on the size of the phage particles. the transducing DNA fragments are generated by faulty excision of a prophage from the host genome. a and b, but not c b and c, but not aarrow_forward
- 1. Transformation refers to .................................. Group of answer choices a.vertical transfer of genetic material b.transfer of genetic material via sex pili c.DNA fragment transferred between live F + donor and recipient cells d.using a virus to transfer DNA fragment e.the uptake of “naked” DNA fragment by competent cells 2. Identify the MISMATCH pair from the following Group of answer choices a.shake tube method :::: for cultivation of aerobic bacteria's b.glycerol uptake :::: facilitated diffusion c. gonorrheae pathogen ::::: prefers low oxygen d.SAB media :::: high salt and sugar with low pH e.defined media :::: simple media f.micronutrients :::: acts as cofactors in bacterial growtharrow_forwardPhage Mu may be integrated into E. coli genomic DNA via Select one: O a. recombination catalyzed by homologous recombination enzymes. O b. conservative transposition O c. integration catalyzed by integrase. d. replicative transpositionarrow_forwarda) Explain semi-conservative modelmof DNA replication b) discuss initiation in DNA replication and explain why it is a tightly controlled process.arrow_forward
- Plasmids are circular extrachromosomal DNA in bacteria that play a role in: d. degradation of viruses a. growth of bacteria b. pathogenicity and antibiotic resistance c. production of antibodiesarrow_forwardThe HIV virus produces copies of its genome through: A. DNA replication B. Reverse transcription followed by integration then normal transcription C. RNA replication from an RNA template D. differential splicingarrow_forwardIn observing replication of prokaryotic plasmids by electron microscopy, it is apparent that: a. replication is initiated at two distinct origins of replication. b. the template strands completely unwind before replication begins. c. replication initiates from a single origin, creating a replication bubble d. the plasmid is linearized prior to replicationarrow_forward
- Which of the following is NOT common to all repair mechanisms? A. Detection of the lesion B. Removal of the damaged DNA sequence/ nucleotide/ base C. Removal of free radicals at the site of injury D. Repair of the lesion E. Involvement of enzymes in removing the lesion and/or repair of the damaged sequence/nucleotide/base.arrow_forwardDuring the late stage T4 phage infection, the phage DNA is synthesized by Select one: а. the DNA polymerase encoded by T4 genome. the ADP-ribosylated DNA polymerase encoded by E. coli genome. O c. the ADP-ribosylated DNA polymerase encoded by T4 genome. d. the DNA polymerase encoded by E. coli genome.arrow_forwardDuring transduction via phage P1,a. any small fragment of the bacterial chromosome may betransferred to another bacterium by a phage.b. only a specific fragment of DNA may be transferred to anotherbacterium by a phage.c. any small fragment of the bacterial chromosome may betransferred during conjugation.d. only a specific fragment of DNA may be transferred duringconjugation.arrow_forward
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