Biology: Life on Earth with Physiology (11th Edition)
11th Edition
ISBN: 9780133923001
Author: Gerald Audesirk, Teresa Audesirk, Bruce E. Byers
Publisher: PEARSON
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Chapter 16, Problem 2FIB
Different versions of the same gene are called ______. These versions arise as a result of changes in the sequence of ______ that form the gene. These changes are known as ______. An Individual with two identical copies of a given gene is described as being ______ for that gene, while an individual with two different versions of that gene is described as ______.
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 16 Solutions
Biology: Life on Earth with Physiology (11th Edition)
Ch. 16.1 - define evolution in terms of concepts from...Ch. 16.1 - define equilibrium population and describe the...Ch. 16.2 - Evolution of a Menace The mutant alleles that...Ch. 16.2 - describe how mutation, gene flow, genetic drift,...Ch. 16.2 - A flu vaccination stimulates your immune system to...Ch. 16.2 - If it were true that mutations do occur in...Ch. 16.2 - Explain how the distribution of genotypes in...Ch. 16.2 - If a population grows large again after a...Ch. 16.2 - Prob. 4TCCh. 16.3 - describe why selection of phenotypes can affect...
Ch. 16.3 - A team of phys clans treated four patients with...Ch. 16.3 - If we studied a population of bighorn sheep and...Ch. 16.3 - explain how competition and predation influence...Ch. 16.3 - When selection is directional, is there any limit...Ch. 16.3 - Prob. 3CYLCh. 16.3 - Microbiologists have discovered that alleles...Ch. 16.3 - compare and contrast directional selection,...Ch. 16 - In North America, the average height of adult...Ch. 16 - The ______ provides a simple mathematical model...Ch. 16 - The alleles responsible for antibiotic resistance...Ch. 16 - What is a gene pool? How would you determine the...Ch. 16 - By the 1940s, the whooping crane population had...Ch. 16 - Different versions of the same gene are called...Ch. 16 - Stabilizing selection on a trait tends to a. make...Ch. 16 - Define equilibrium population. Outline the...Ch. 16 - An organisms ______ refers to the specific alleles...Ch. 16 - An adaptation is a. any trait that arises from a...Ch. 16 - How does population size affect the likelihood of...Ch. 16 - A random form of evolution is called ________....Ch. 16 - Which of the following statements about mutations...Ch. 16 - If you measured the allele frequencies of a gene...Ch. 16 - Competition is most Intense between members of...Ch. 16 - Genetic drift occurs a. when different phenotypes...Ch. 16 - People like to say that you cant prove a negative....Ch. 16 - The evolutionary fitness of an organism is...Ch. 16 - Describe the three ways in which natural selection...Ch. 16 - What is sexual selection? How is sexual selection...
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- Which of the following is not a strength of using 16S rRNA for phylogenetic analyses? OA. It's cheap OB. It's easy to do C. It can be used to identify all the way down to the strain level OD. Both A & B OE. None of the abovearrow_forwardWhy are molecular approaches important to the field of microbial taxonomy and phylogeny? Phylogenetic inferences based on molecular approaches provide the most robust analysis of microbial evolution currently available. It allows for the collection of a large and accurate dataset from many organisms Almost no fossil record was left by microbes when compared to plants and animals All of the above None of the abovearrow_forwardYou will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have already cultured it and gone through the plate isolation procedure. Before you ship your samples off for sequencing, you want to do one final check of the A260 ratios. You get back the following ratios: A260/280 ratio is 1.89; A260/230 is 2.01. These ratios are close enough to the accepted "pure" values so they could be considered "pure" and mostly (if not completely) free of contaminants from the PCR process. True Falsearrow_forward
- You will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. After receiving your sequence back from the sequencing lab, you feel that you have, in fact, discovered and isolated a new species. You ask a fellow labmate about how you should proceed, and he tells you the following is the proper way to introduce a new species for recognition: Cultures have to be sent to international culture collections. Then a paper must be published describing the new organism and providing a genus and species name. You recall learning about this in your Microbiology course in college. Is this information from your colleague true or false? True Falsearrow_forwardis often a good indication of phylogenetic relatedness in phenotypes. Life-cycle patterns Cleavage patterns O Gene expression O Morphological similarityarrow_forwardWhich of the following is a weakness of using 16S rRNA for phylogenetic analyses? It can only go down to the family and genus levels It takes months to complete O Both of the above O None of the abovearrow_forward
- An unrooted tree containing ten unrelated species can become rooted by adding a descendant group related to two of the species. an unrelated outgroup. O a distantly related outgroup. O a descendant related to only one of the species.arrow_forwardWhat is the most appropriate purpose of building a phylogenetic tree? They look awesome You can use a tree to compare morphological characteristics of organisms It can be used to establish and analyze evolutionary relationships between species All of the abovearrow_forwardWhich of the following sequencing techniques can identify down to the strain level? O Multilocus sequence typing Genomic fingerprinting Whole genome sequencing OSNP analysis All of the abovearrow_forward
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