Chapter 15.6, Problem B15.4P

Interpretation Introduction

Interpretation:

If a DNA fragment with the base sequence $\text{TACAGGTTCAGT}$ is analysing the number of complementary chain pieces obtained in the tube containing $\text{ddATP}$ and the tube containing $\text{ddCTP}$ has to be given.

Concept Introduction:

When $\text{ddNTP}$ is added to the growing DNA chain, it stops the formation of phosphodiester bonds as polymerization of the chain is stopped.  The $\text{ddATP}$ stops the chain at base T and base T encounters by base A and $\text{ddCTP}$ stops the chain at base G and base G encounters by base C.