A. The location of silencer and enhancer. B. The reason behind the absence of silencer in the pancreatic cell. D. The reason behind no gene expression in kidney cell. Introduction: A genetic technique called promoter bashing, identifies genetic sequences and promote regulation of gene transcription. A gene which is usually expressed in pancreatic cells is selected for promoter bashing where different segments of the upstream region labeled A to D are deleted, and DNA(Deoxyribonucleic acid) is introduced into the pancreatic cells or kidney cells. The percentage of beta-galactosidase activity is measured in both pancreatic cells and kidney cells.

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Chapter 15, Problem 5EQ

Summary Introduction

To review:

A. The location of silencer and enhancer.

B. The reason behind the absence of silencer in the pancreatic cell.

D. The reason behind no gene expression in kidney cell.

Introduction:

A genetic technique called promoter bashing, identifies genetic sequences and promote regulation of gene transcription. A gene which is usually expressed in pancreatic cells is selected for promoter bashing where different segments of the upstream region labeled A to D are deleted, and DNA(Deoxyribonucleic acid) is introduced into the pancreatic cells or kidney cells. The percentage of beta-galactosidase activity is measured in both pancreatic cells and kidney cells.

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