Genetics: Analysis and Principles
6th Edition
ISBN: 9781259616020
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Chapter 15, Problem 12CONQ
Summary Introduction
To review:
A. The prevention of the function of the glucocorticoid receptor.
B. The enzyme required to inhibit the transcription activation by CREB protein.
Introduction:
The CREB protein(cyclic adenosine monophosphate response element binding protein) and the glucocorticoid receptors work asactivators of transcription of genes. Specific sites on DNA (deoxyribonucleic acid)gets bind with CREB protein and promote the transcription of target genes. They regulate the gene expression in a cell.
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Check out a sample textbook solutionStudents have asked these similar questions
Which of the following is the most likely result of a mutation in the lac operator that prevents release of the depressor protein?
Choose 1 answer:
(A)The lactose permease protein will be produced, even in the absence of lactose.
(B) Transcription of the structural genes will be blocked, even in the presence of lactose.
(C) The catabolite activator protein will be bound to the CAP site, even in the presence of glucose.
(D) Expression of the lacI gene will be repressed, even in the absence of glucose.
Now read this abstract from a 2013 journal article What is the authors' explanation of how Gal80p works?
Note UASG from the question above is the same as UASGAL
The DNA-binding transcriptional activator Gal4 and its regulators Gal80 and Gal3 constitute a galactose-responsive switch for
the GAL genes of Saccharomyces cerevisiae. Gal4 binds to GAL gene UASGAL. (upstream activation sequence in GAL gene pro-
moter) sites as a dimer via its N-terminal domain and activates transcription via a C-terminal transcription activation domain
(AD). In the absence of galactose, a Gal80 dimer binds to a dimer of Gal4, masking the Gal4AD. Galactose triggers Gal3-Gal80
interaction to rapidly initiate Gal4-mediated transcription activation. Just how Gal3 alters Gal80 to relieve Gals0 inhibition of
Gal4 has been unknown, but previous analyses of Gal80 mutants suggested a possible competition between Gal3-Gal80 and
Gal80 self-association interactions. Here we assayed Gal80-Gal80 interactions and tested for…
If I have discovered a mutation in the glucocorticoid reception that does not prevent thebinding of glucocorticoid hormone to the protein but prevents the ability of the receptor toactivate transcription, what are all of the possible defects that may explain why transcriptioncannot be activated?
Chapter 15 Solutions
Genetics: Analysis and Principles
Ch. 15.1 - 1. Combinatorial control refers to the phenomenon...Ch. 15.1 - 2. A regulatory transcription factor protein...Ch. 15.1 - 3. A bidirectional enhancer has the following...Ch. 15.1 - 4. Regulatory transcription factors can be...Ch. 15.2 - 1. A chromatin-remodeling complex may
a. change...Ch. 15.2 - Prob. 2COMQCh. 15.2 - 3. Which of the following characteristics is...Ch. 15.2 - 4. Transcriptional activation of eukaryotic genes...Ch. 15.3 - How can methylation affect transcription? a. It...Ch. 15.3 - 2. The process in which completely unmethylated...
Ch. 15.4 - Prob. 1COMQCh. 15.5 - The overall goal of the ENCODE Project is a. to...Ch. 15.6 - The binding of iron regulatory protein (IRP) to...Ch. 15 - Discuss the common points of control in eukaryotic...Ch. 15 - 2. Discuss the structure and function of...Ch. 15 - 3. What is meant by the term transcription factor...Ch. 15 - What are the functions of transcriptional...Ch. 15 - 5. Is each of the following statements true or...Ch. 15 - 6. Transcription factors usually contain one or...Ch. 15 - Prob. 7CONQCh. 15 - Prob. 8CONQCh. 15 - 9. Let’s suppose a mutation in the glucocorticoid...Ch. 15 - Prob. 10CONQCh. 15 - Prob. 11CONQCh. 15 - Prob. 12CONQCh. 15 - 13. Transcription factors such as the...Ch. 15 - An enhancer, located upstream from a gene, has the...Ch. 15 - 15. The DNA-binding domain of each CREB protein...Ch. 15 - The gene that encodes the enzyme called tyrosine...Ch. 15 - Prob. 17CONQCh. 15 - 18. What is a histone variant?
Ch. 15 - Prob. 19CONQCh. 15 - 20. What is meant by the term histone code? With...Ch. 15 - Prob. 21CONQCh. 15 - Histones are thought to be displaced as RNA...Ch. 15 - 23. What is an insulator? Describe two different...Ch. 15 - 24. What is DNA methylation? When we say that DNA...Ch. 15 - Lets suppose that a vertebrate organism carries a...Ch. 15 - 26. What is a CpG island? Where would you expect...Ch. 15 - Describe how the binding of iron regulatory...Ch. 15 - 1. Briefly describe the method of chromatin...Ch. 15 - Researchers can isolate a sample of cells, such as...Ch. 15 - Prob. 3EQCh. 15 - Prob. 4EQCh. 15 - Prob. 5EQCh. 15 - 6. As described in Chapter 21, an electrophoretic...Ch. 15 - Prob. 7EQCh. 15 - 1. Explain how DNA methylation could be used to...Ch. 15 - 2. Enhancers can occur almost anywhere in DNA and...
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- What is meant by the term transcription factor modulation? List three general ways this can occur.arrow_forwardExplain why it is advantageous for transcriptionalregulatory proteins to be multimeric and for theirbinding sites to be clustered.arrow_forwardIf the lacl gene is mutated so that the repressor protein no longer binds to lactose, what will be the effect on the expression of B-galactosidase in lactose's presence and absence? Explain. If the promoter for lacl is mutated so that the expression of the repressor increases, what will be the effect on the expression of B-galactosidase in the presence and absence of lactose? Explain. D. (Extremely tricky question!) Describe the behavior of the lac operon assuming that the lacl gene has been mutated so that the repressor now binds to DNA in the presence of lactose but cannot bind to DNA in the absence of lactose.arrow_forward
- CTP synthetase is allosterically activated by GTP. What function might this play in the cell?arrow_forwardThe retinoblastoma tumor suppressor gene Rb (RB1) codes forthe retinoblastoma protein (pRB). pRB prevents the progression of the cell cycle through G1 if DNA has been damaged. Itdoes so in part because it binds a transcription-activatingdimer referred to as E2F-DP. The pRB-E2F/DP complex recruits a histone deacetylase to chromatin. Explain.arrow_forwardThe concentration of free glutamine affects transcription of the enzyme glutamine synthetase in E. coli. Describe the mechanism of this effect.arrow_forward
- Please arrangearrow_forwardTranscriptional regulators are proteins that bind to promoters (at the 5’ flanking regions of genes) to regulate their transcription. Assume that a particular transcription regulator normally promotes transcription of gene X that codes for a membrane transport protein. If a mutation makes this transcription regulator nonfunctional, would the resulting phenotype be similar to a deleterious mutation in the membrane transporter itself? Why or why not?arrow_forwardThe binding of a small effector molecule, protein-protein interactions, and covalent modifications are three common ways to modulate the activities of transcription factors. Which of these three mechanisms are used by steroid receptors and by the CREB protein?arrow_forward
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