(a) Interpretation: The affect of the decreasing amount of [ Mg 2 + ] over the value of Δ G for the hydrolysis of ATP molecules needs to be explained. Concept introduction: A thermodynamic potential that is utilized in the calculation of the highest reversible function taking place in the thermodynamic system at the constant value pressure and temperature is known as Gibbs-free energy. It is denoted by Δ G ° .

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Answer 1

Question

Chapter 15, Problem 37P

Interpretation Introduction

(a)

Interpretation:

The affect of the decreasing amount of [Mg2+] over the value of ΔG for the hydrolysis of ATP molecules needs to be explained.

Concept introduction:

A thermodynamic potential that is utilized in the calculation of the highest reversible function taking place in the thermodynamic system at the constant value pressure and temperature is known as Gibbs-free energy. It is denoted by ΔG°.

Interpretation Introduction

(b)

Interpretation:

The effect of the decreasing concentration of [Mg2+] over the value of ΔG for the hydrolysis of ATP moleculesneeds to be explained.

Concept introduction:

A thermodynamic potential that is utilized in the calculation of the highest reversible function taking place in the thermodynamic system at the constant value pressure and temperature is known as Gibbs-free energy. It is denoted by ΔG°.

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The beta-lactamase hydrolyzes the lactam-ring in penicillin. Describe the mechanism of hydrolysis, insuring to include the involvement of S, D, & K in the reaction sequence. Please help

To map the active site of beta-lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Why doesn't D in this hexapeptide not participate in the hydrolysis of the beta-lactam ring even though S, K, and D are involved in the catalyst?

To map the active site of -lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Using the experimental results described above derive the primary sequence of the active site hexapeptide. Please help!

Answer 2

Question

Chapter 15, Problem 37P

Interpretation Introduction

(a)

Interpretation:

The affect of the decreasing amount of [Mg2+] over the value of ΔG for the hydrolysis of ATP molecules needs to be explained.

Concept introduction:

A thermodynamic potential that is utilized in the calculation of the highest reversible function taking place in the thermodynamic system at the constant value pressure and temperature is known as Gibbs-free energy. It is denoted by ΔG°.

Interpretation Introduction

(b)

Interpretation:

The effect of the decreasing concentration of [Mg2+] over the value of ΔG for the hydrolysis of ATP moleculesneeds to be explained.

Concept introduction:

A thermodynamic potential that is utilized in the calculation of the highest reversible function taking place in the thermodynamic system at the constant value pressure and temperature is known as Gibbs-free energy. It is denoted by ΔG°.

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Answer 3

Question

Chapter 15, Problem 37P

Interpretation Introduction

(a)

Interpretation:

The affect of the decreasing amount of [Mg2+] over the value of ΔG for the hydrolysis of ATP molecules needs to be explained.

Concept introduction:

A thermodynamic potential that is utilized in the calculation of the highest reversible function taking place in the thermodynamic system at the constant value pressure and temperature is known as Gibbs-free energy. It is denoted by ΔG°.

Interpretation Introduction

(b)

Interpretation:

The effect of the decreasing concentration of [Mg2+] over the value of ΔG for the hydrolysis of ATP moleculesneeds to be explained.

Concept introduction:

A thermodynamic potential that is utilized in the calculation of the highest reversible function taking place in the thermodynamic system at the constant value pressure and temperature is known as Gibbs-free energy. It is denoted by ΔG°.

Expert Solution & Answer

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Answer 4

Question

Chapter 15, Problem 37P

Interpretation Introduction

(a)

Interpretation:

The affect of the decreasing amount of [Mg2+] over the value of ΔG for the hydrolysis of ATP molecules needs to be explained.

Concept introduction:

A thermodynamic potential that is utilized in the calculation of the highest reversible function taking place in the thermodynamic system at the constant value pressure and temperature is known as Gibbs-free energy. It is denoted by ΔG°.

Interpretation Introduction

(b)

Interpretation:

The effect of the decreasing concentration of [Mg2+] over the value of ΔG for the hydrolysis of ATP moleculesneeds to be explained.

Concept introduction:

A thermodynamic potential that is utilized in the calculation of the highest reversible function taking place in the thermodynamic system at the constant value pressure and temperature is known as Gibbs-free energy. It is denoted by ΔG°.

Expert Solution & Answer

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Answer 5

Chapter 15, Problem 37P

Interpretation Introduction

(a)

Interpretation:

The affect of the decreasing amount of [Mg2+] over the value of ΔG for the hydrolysis of ATP molecules needs to be explained.

Concept introduction:

A thermodynamic potential that is utilized in the calculation of the highest reversible function taking place in the thermodynamic system at the constant value pressure and temperature is known as Gibbs-free energy. It is denoted by ΔG°.

Interpretation Introduction

(b)

Interpretation:

The effect of the decreasing concentration of [Mg2+] over the value of ΔG for the hydrolysis of ATP moleculesneeds to be explained.

Concept introduction:

A thermodynamic potential that is utilized in the calculation of the highest reversible function taking place in the thermodynamic system at the constant value pressure and temperature is known as Gibbs-free energy. It is denoted by ΔG°.

Answer 6

Chapter 15, Problem 37P

Interpretation Introduction

(a)

Interpretation:

The affect of the decreasing amount of [Mg2+] over the value of ΔG for the hydrolysis of ATP molecules needs to be explained.

Concept introduction:

A thermodynamic potential that is utilized in the calculation of the highest reversible function taking place in the thermodynamic system at the constant value pressure and temperature is known as Gibbs-free energy. It is denoted by ΔG°.

Answer 7

Chapter 15, Problem 37P

Interpretation Introduction

(a)

Interpretation:

The affect of the decreasing amount of [Mg2+] over the value of ΔG for the hydrolysis of ATP molecules needs to be explained.

Concept introduction:

A thermodynamic potential that is utilized in the calculation of the highest reversible function taking place in the thermodynamic system at the constant value pressure and temperature is known as Gibbs-free energy. It is denoted by ΔG°.

Answer 8

Interpretation Introduction

(b)

Interpretation:

The effect of the decreasing concentration of [Mg2+] over the value of ΔG for the hydrolysis of ATP moleculesneeds to be explained.

Concept introduction:

A thermodynamic potential that is utilized in the calculation of the highest reversible function taking place in the thermodynamic system at the constant value pressure and temperature is known as Gibbs-free energy. It is denoted by ΔG°.

Answer 9

Interpretation Introduction

(b)

Interpretation:

The effect of the decreasing concentration of [Mg2+] over the value of ΔG for the hydrolysis of ATP moleculesneeds to be explained.

Concept introduction:

A thermodynamic potential that is utilized in the calculation of the highest reversible function taking place in the thermodynamic system at the constant value pressure and temperature is known as Gibbs-free energy. It is denoted by ΔG°.

Answer 10

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Answer 11

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Answer 12

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Answer 13

Ch. 15 - Prob. 1P Ch. 15 - Prob. 2P Ch. 15 - Prob. 3P Ch. 15 - Prob. 4P Ch. 15 - Prob. 5P Ch. 15 - Prob. 6P Ch. 15 - Prob. 7P Ch. 15 - Prob. 8P Ch. 15 - Prob. 9P Ch. 15 - Prob. 10P

Solution Summary: The author analyzes the effect of Gibbs-free energy, which is denoted by Delta G°, on hydrolysis of ATP molecules.

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