EBK MICROBIOLOGY:W/DISEASES BY BODY...-
5th Edition
ISBN: 9780134608242
Author: BAUMAN
Publisher: PEARSON
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Textbook Question
Chapter 15, Problem 15MTF
Modified True/False
15. __________________ NETs are webs produced by neutrophils to trap microbes.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Based on your results, which suspect's DNA best matches the DNA found at the crime scene?
Chapter 15 Solutions
EBK MICROBIOLOGY:W/DISEASES BY BODY...-
Ch. 15 - Prob. 1TMWCh. 15 - Some strains of Staphylococcus aureus produce...Ch. 15 - Evaluating an Abnormal CBC Roger Brown, an African...Ch. 15 - The Stealth Invader Tim is often seen walking...Ch. 15 - Prob. 3TMWCh. 15 - Phagocytes of the epidermis are called _________....Ch. 15 - Mucus-secreting membranes are found in ________....Ch. 15 - The complement system involves _________. a. the...Ch. 15 - The alternative complement activation pathway...Ch. 15 - Which of the complement fragments is inflammatory?...
Ch. 15 - The type of interferon present late in an...Ch. 15 - Interferons ________. a. do not protect the cell...Ch. 15 - Prob. 8MCCh. 15 - Toll-like receptors (TLRs) act to ________. a....Ch. 15 - Prob. 10MCCh. 15 - Modified True/False 1. _______ The surface cells...Ch. 15 - Modified True/False 2. _______ The surface cells...Ch. 15 - Prob. 3MTFCh. 15 - Prob. 4MTFCh. 15 - Prob. 5MTFCh. 15 - Prob. 6MTFCh. 15 - Prob. 7MTFCh. 15 - Prob. 8MTFCh. 15 - Prob. 9MTFCh. 15 - Prob. 10MTFCh. 15 - Prob. 11MTFCh. 15 - Prob. 12MTFCh. 15 - Prob. 13MTFCh. 15 - Modified True/False 14. ___________________...Ch. 15 - Modified True/False 15. __________________ NETs...Ch. 15 - Prob. 1MCh. 15 - Write the letter of the description that applies...Ch. 15 - Label the steps of phagocytosis.Ch. 15 - Prob. 2VICh. 15 - In order for a pathogen to cause disease, what...Ch. 15 - How does a phagocyte know it is in contact with a...Ch. 15 - Give three characteristics of the epidermis that...Ch. 15 - What is the role of Toll-like receptors in innate...Ch. 15 - Describe the classical complement cascade pathway...Ch. 15 - Prob. 6SACh. 15 - John received a chemical burn on his arm and was...Ch. 15 - What might happen to someone whose body did not...Ch. 15 - Prob. 3CTCh. 15 - Prob. 4CTCh. 15 - There are two kinds of agranulocytes in the...Ch. 15 - A patient has a genetic disorder that prevents him...Ch. 15 - Prob. 7CTCh. 15 - Scientists can raise germ-free animals in axenic...Ch. 15 - Prob. 9CTCh. 15 - Scientists are interested in developing...Ch. 15 - A medical laboratory scientist argues that...Ch. 15 - A patient has a genetic disorder that makes it...Ch. 15 - Prob. 13CTCh. 15 - Prob. 14CTCh. 15 - Using the following terms, fill in the following...
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