Biology: Life on Earth (11th Edition)
11th Edition
ISBN: 9780134168296
Author: Gerald Audesirk, Teresa Audesirk, Bruce E. Byers
Publisher: PEARSON
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Chapter 14, Problem 5FTB
Summary Introduction
To review:
The given blank space in the statement, “pieces of deoxyribonucleic acid (DNA) can be separated according to size by a process known as __________________. The identity of a specific sample of DNA is usually determined by binding a synthetic piece of DNA called a(n) __________________, which binds to the sample DNA by __________________.”
Introduction:
For the proper detection of particular genes in the DNA strands, it is necessary for the separation of the DNA strands from each other. The separation is usually done on the basis of their size. The technique by which the DNA strands are separated is called gel electrophoresis.
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Chapter 14 Solutions
Biology: Life on Earth (11th Edition)
Ch. 14 - Which of the following is not true of a single...Ch. 14 - Imagine you are looking at a DNA profile that...Ch. 14 - 3. Which of the following is not a commonly used...Ch. 14 - Prob. 4MCCh. 14 - 5. DNA cloning is
a. making multiple genetically...Ch. 14 - Are organisms that contain DNA that has been...Ch. 14 - 2._________ is the process whereby bacteria pick...Ch. 14 - 3. The______ is a technique for multiplying DNA in...Ch. 14 - Matching DNA samples in forensics uses a specific...Ch. 14 - Prob. 5FTB
Ch. 14 - Prob. 1RQCh. 14 - 2. What is a plasmid? How are plasmids involved in...Ch. 14 - 3. What is a restriction enzyme? How can...Ch. 14 - 4. Describe the polymerase chain reaction.
Ch. 14 - What is a short tandem repeat? How are short...Ch. 14 - How does gel electrophoresis separate pieces of...Ch. 14 - How are DNA probes used to identify specific...Ch. 14 - Prob. 8RQCh. 14 - Describe several uses of genetic engineering in...Ch. 14 - Prob. 10RQCh. 14 - 1. As you may know, many insects have evolved...Ch. 14 - 2. All children born with X-linked SCID are boys....
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- In E. coli, the DNA helicase is loaded onto the DNA by __________ and activated when __________ binds the helicase. Question 24 options: DnaC; DnaG DnaA; DnaB DnaG; DnaC DnaB; DnaA DnaC; DnaBarrow_forwardYou accompany a friend to the doctor with their young child. The doctor says, "your child has a heritable change in a DNA sequence that codes for a polypeptide chain. Your friend looks at you helplessly, so you translate: Jimmy has a ______ in a _______?arrow_forwardIn analyzing the base composition of a DNA sample, a student loses the information on pyrimidine content. The purine content is A = 29% and G = 21%. Using Chargaff’s rule, reconstruct the missing data and list the base composition of the DNA sample. (Include a brief explanation of how you got your results).arrow_forward
- A DNA strand was sequenced using the Sanger method (https://www.youtube.com/watch?v=KTstRrDTmWI). The reaction tube contained the DNA strand, fluorescently labelled dideoxynucleotide triphosphates (ddATP – yellow, ddGTP – green, ddCTP – blue, ddTTP - red), deoxynucleotide triphosphates, DNA polymerase, or its Klenow fragment. Synthesis of DNA is allowed to proceed, and the results are shown on the right: 15 14 13 12 11 10 (a) What is the sequence of the copy and the template strands? (b) If the template strand were in the 5'-3' direction, what will be the sequence of the DNA copy? Nucleotide Lengtharrow_forwardEach new double helix will contain ___________ strand of DNA from the parent strand and ____________ strand of newly created DNA.arrow_forwardIn order to determine the purity of a DNA sample. spectrophotometry can be carried out at calculated, and a number between to measure DNA, and to measure protein. The ratio is then indicates higher purity. 280 nm; 260 nm; A280/A260; 0.5-1 260 nm; 280 nm; A260/A280; 0.5-1 260 nm; 280 nm; A280/A260; 1.5-2 260 nm; 280 nm; A260/A280; 1.5-2arrow_forward
- We often describe the shape of DNA as aarrow_forwardDNA is an attractive option for long-term data storage because hydrogen bonding between single strands of DNA are easy to separate - True or Falsearrow_forwardA student was analyzing the base composition of a DNA sample, but has lost all the data except the content of Cytosine, which is 20%. Based on this information, reconstruct the missing data and list the base composition of the DNA sample.arrow_forward
- A portion of one strand of DNA has the sequence 5′ AATGGCTTA 3′. If this strand is used as a template for DNA replication, write the sequence of the newly synthesized strand in the direction from left to right in which it will be synthesized.arrow_forwardTrue or false: The synthesis of a new strand of DNA is initiated using a DNA primer?arrow_forwardproteins are denatured and eliminated during the DNA extraction process. how can we determine the purity of our DNA sample?arrow_forward
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