Biology: Life on Earth (11th Edition)
11th Edition
ISBN: 9780134168296
Author: Gerald Audesirk, Teresa Audesirk, Bruce E. Byers
Publisher: PEARSON
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Chapter 14, Problem 1RQ
Summary Introduction
To review:
The three natural forms of genetic recombination and its comparison with recombinant deoxyribonucleic acid (DNA) technology.
Introduction:
The three natural forms of genetic recombination are sexual reproduction, viral infection, and bacterial transformation. The recombinant DNA technology is the combination of two different molecules of DNA from different species in a host organism in order to produce new genetic combination.
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Describe the basic principles behind recombinant DNA technology.
Name the five key tools for accomplishing the tasks of recombinant DNA technology.also mention the function of each tool.
How can site-specific recombination be used in recombinant DNA technology? Explain with an example
Chapter 14 Solutions
Biology: Life on Earth (11th Edition)
Ch. 14 - Which of the following is not true of a single...Ch. 14 - Imagine you are looking at a DNA profile that...Ch. 14 - 3. Which of the following is not a commonly used...Ch. 14 - Prob. 4MCCh. 14 - 5. DNA cloning is
a. making multiple genetically...Ch. 14 - Are organisms that contain DNA that has been...Ch. 14 - 2._________ is the process whereby bacteria pick...Ch. 14 - 3. The______ is a technique for multiplying DNA in...Ch. 14 - Matching DNA samples in forensics uses a specific...Ch. 14 - Prob. 5FTB
Ch. 14 - Prob. 1RQCh. 14 - 2. What is a plasmid? How are plasmids involved in...Ch. 14 - 3. What is a restriction enzyme? How can...Ch. 14 - 4. Describe the polymerase chain reaction.
Ch. 14 - What is a short tandem repeat? How are short...Ch. 14 - How does gel electrophoresis separate pieces of...Ch. 14 - How are DNA probes used to identify specific...Ch. 14 - Prob. 8RQCh. 14 - Describe several uses of genetic engineering in...Ch. 14 - Prob. 10RQCh. 14 - 1. As you may know, many insects have evolved...Ch. 14 - 2. All children born with X-linked SCID are boys....
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- Briefly explain about computer-automated high-throughput DNA sequencing ?arrow_forwardPractical applications of recombinant DNA technology include the: 1. Efficient production of useful proteins and development of a new type of vaccines 2. Creation of novel genotypes for the synthesis of economically important molecules. 3. Generation of DNA and RNA sequences for use in medical diagnosis. 4. all of the above WHICH OF THE FOLLOWING TECHNIQUE IS NOT USED TO MANIPULATE THE GENOME 1. siRNA 2. ZF (zinc finger) 3. TALE (transcription activator light effector) 4. CRISPR COMMON DISEASE- COMMON VARIANT HYPOTHESIS STATES THAT 1. common disorders are likely influences by genetic variation that is also common in the population 2. if an SNP produced a deleterious mutation and changes amino acid sequence in 40% of individuals- it will produce disease phenotype in 40% individual 3. A SNP produce little change in gene expression- it will produce slight increase in risk/ manifestation of disease phenotype 4. all of the above WHAT IS TRUE REGARDING GENE ORGANIZATION IN THE NUCLEUS…arrow_forwarda) Restriction enzymes are essential tools in genetic engineering. Discuss with diagrams b)Define the term genetic engineering and in a tabular form outline the differences between genetic recombination and genetic engineeringarrow_forward
- Describe at least two safety issues associated with DNA technologyand explain how these issues are being addressed.arrow_forwarddescribe how DNA shuffling and molecular breeding are used to generate hybrid genes and the advantages and disadvantages of this method for muthagenesis.arrow_forwardHigh Frequency Recombination results in which of the following? O 1) Plasmid DNA incorporated into the cell 2) Genomic DNA incorporated into the cell 3) Viral DNA incorporated into the cell 4) Plasmid and genomic DNA from the donor cell is incorporated into the recipient cell's genome 5) The movement of genes within the cell through homologous recombinationarrow_forward
- Describe how the processes of recombinant DNA technology are related to one another.arrow_forwardCompare and contrast at least three considerations in terms of methods and/or requirements in introducing recombinant DNA in plant calli versus animal cell lines. Provide at least three criteria of comparisonarrow_forwardDescribe the technique of in situ hybridization. Explain how it can be used to map genes.arrow_forward
- List the steps involved in making recombinant DNAarrow_forwardConsider the following experiment. First, large populations of two mutant strains of Escherichia coli are mixed, each requiring a different, single amino acid. After plating them onto a minimal medium, 45 colonies grew. Which of the following may explain this result? A) The colonies may be due to back mutation (reversion). B) The colonies may be due to recombination. C) Either A or B is possible. D) Neither A nor B is possible.arrow_forwardRecombinant DNA Technology Provide a detailed explanation how DNA structure is exploited for the following techniques DNA Sequencing Molecular Cloning Polymerase Chain Reaction (PCR)arrow_forward
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genetic recombination strategies of bacteria CONJUGATION, TRANSDUCTION AND TRANSFORMATION; Author: Scientist Cindy;https://www.youtube.com/watch?v=_Va8FZJEl9A;License: Standard youtube license