Biology: Life on Earth (11th Edition)
11th Edition
ISBN: 9780134168296
Author: Gerald Audesirk, Teresa Audesirk, Bruce E. Byers
Publisher: PEARSON
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Chapter 14, Problem 2RQ
What is a plasmid? How are plasmids involved in bacterial transformation?
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. Consider a base substitution mutation that occurred in a DNA sequence that resulted in a change in the
encoded protein from the amino acid glutamic acid to aspartic acid. Normally the glutamic acid amino acid
is located on the outside of the soluble protein but not near an active site.
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A. What type of mutation occurred?
O-H
B. What 2 types of chemical bonds are found in the R-groups
of each amino acid? The R groups are shaded.
CH2
CH2
CH2
H2N-C-COOH
H2N-C-COOH
1
H
Glutamic acid
H
Aspartic acid
C. What 2 types of bonds could each R-group of each of these amino acids form with other molecules?
D. Consider the chemical properties of the two amino acids and the location of the amino acid in the
protein. Explain what effect this mutation will have on this protein's function and why.
engineered constructs that consist of hollow fibers are acting as synthetic capillaries, around which cells have been loaded. The cellular space around a single fiber can be modeled as if it were a Krogh tissue cylinder. Each fiber has an outside “capillary” radius of 100 µm and the “tissue” radius can be taken as 200 µm. The following values apply to the device:R0 = 20 µM/secaO2 = 1.35 µM/mmHgDO2,T = 1.67 x 10-5 cm2/secPO2,m = 4 x 10-3 cm/secInstead of blood inside the fibers, the oxygen transport and tissue consumption are being investigated by usingan aqueous solution saturated with pure oxygen. As a result, there is no mass transfer resistance in the synthetic“capillary”, only that due to the membrane itself. Rather than accounting for pO2 variations along the length ofthe fiber, use an average value in the “capillary” of 130 mmHg.Is the tissue fully oxygenated?
Chapter 14 Solutions
Biology: Life on Earth (11th Edition)
Ch. 14 - Which of the following is not true of a single...Ch. 14 - Imagine you are looking at a DNA profile that...Ch. 14 - 3. Which of the following is not a commonly used...Ch. 14 - Prob. 4MCCh. 14 - 5. DNA cloning is
a. making multiple genetically...Ch. 14 - Are organisms that contain DNA that has been...Ch. 14 - 2._________ is the process whereby bacteria pick...Ch. 14 - 3. The______ is a technique for multiplying DNA in...Ch. 14 - Matching DNA samples in forensics uses a specific...Ch. 14 - Prob. 5FTB
Ch. 14 - Prob. 1RQCh. 14 - 2. What is a plasmid? How are plasmids involved in...Ch. 14 - 3. What is a restriction enzyme? How can...Ch. 14 - 4. Describe the polymerase chain reaction.
Ch. 14 - What is a short tandem repeat? How are short...Ch. 14 - How does gel electrophoresis separate pieces of...Ch. 14 - How are DNA probes used to identify specific...Ch. 14 - Prob. 8RQCh. 14 - Describe several uses of genetic engineering in...Ch. 14 - Prob. 10RQCh. 14 - 1. As you may know, many insects have evolved...Ch. 14 - 2. All children born with X-linked SCID are boys....
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