Biochemistry
Biochemistry
9th Edition
ISBN: 9781305961135
Author: Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher: Cengage Learning
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Chapter 14, Problem 1RE
Interpretation Introduction

Interpretation:

The genetic material of viruses is to be stated.

Concept introduction:

Deoxyribonucleic acid is made up of two chains that form a double helix structure. DNA is used in the development, growth, reproduction, and functioning of a living organism.

Ribonucleic acid is a polymeric molecule, which is used in coding, decoding, and regulation and explains the nature of genes.

Expert Solution & Answer
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Answer to Problem 1RE

Solution: Some viruses contain DNA and some viruses contain RNA and, in a few cases, the viral genome is single-stranded.

Explanation of Solution

Viruses are nanoparticles, which are made up of nucleic acid and proteins. The nucleic acid that may be single-stranded or double-stranded contains a chromosome, in which the core of the genetic material DNA and RNA is present and it is placed within the protein, which simulates only in an affected host cell.

Conclusion

DNA and RNA are the core of the genetic material of viruses and, in some cases, the nucleic acid of the virus is single stranded or double stranded.

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The beta-lactamase hydrolyzes the lactam-ring in penicillin. Describe the mechanism  of hydrolysis, insuring to include the involvement of S, D, & K in the reaction sequence. Please help
To map the active site of beta-lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Why doesn't D in this hexapeptide not participate in the hydrolysis of the beta-lactam ring even though S, K, and D are involved in the catalyst?
To map the active site of -lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine.  Using the experimental results described above derive the primary sequence of the active site hexapeptide. Please help!
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