Campbell Biology: Concepts & Connections (8th Edition)
8th Edition
ISBN: 9780321885326
Author: Jane B. Reece, Martha R. Taylor, Eric J. Simon, Jean L. Dickey, Kelly A. Hogan
Publisher: PEARSON
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Textbook Question
Chapter 12, Problem 4TYK
A paleontologist has recovered a tiny bit of organic material from the 400-year-old preserved skin of an extinct dodo. She would like to compare DNA from the sample with DNA from living birds. Which of the following would be most useful for increasing the amount of DNA available for testing?
a. restriction fragment analysis
b. polymerase chain reaction
c. molecular probe analysis
d. electrophoresis
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Can you help me with this question, please? What are the advantages of qPCR (RT-PCR) compared to conventional PCR? Choose all that apply
a. human error is reduced as there are fewer human interactions with the samples
b. you can visualize the results as the process is running
c. samples can be compared as to the amount of template DNA in the original sample
d. more samples can be run in a day by one person
Given the situation: A paleontologist has recovered a tiny bit of organic material fromthe 400-year-old preserved skin of an extinct dodo. She would like to compare DNA from the sample with DNA from living birds.Which of the following would be most useful for increasing theamount of DNA available for testing?
Option a.) restriction fragment analysis
Option b.) polymerase chain reaction
Option c.) electrophoresis
After running PCR on your raw milk colony, you run your product on an agarose gel and see multiple bands
coming from a single sample. Assuming there was no contamination in the set up of your PCR reaction, what
could have happened?
a. the primers were not specific enough
b. the PCR reaction was run for too long
c. you forgot to add primers
d. the PCR annealing temperature was too high
e. the master mix lacked Taq enzyme
f. both a and d
Chapter 12 Solutions
Campbell Biology: Concepts & Connections (8th Edition)
Ch. 12 - Imagine you have found a small quantity of DNA....Ch. 12 - Which of the following would be considered a...Ch. 12 - The DNA profiles used as evidence in a murder...Ch. 12 - A paleontologist has recovered a tiny bit of...Ch. 12 - How many genes are there in a human sperm cell? a....Ch. 12 - When a typical restriction enzyme cuts a DNA...Ch. 12 - Why does DNA profiling rely on comparing specific...Ch. 12 - Recombinant DNA techniques are used to...Ch. 12 - A biochemist hopes to find a gene in human cells...Ch. 12 - Prob. 10TYK
Ch. 12 - Explain how you might engineer E. coli to produce...Ch. 12 - What is left for genetic researchers to do now...Ch. 12 - Today, it is fairly easy to make transgenic plants...Ch. 12 - In the not-too-distant future, gene therapy may be...Ch. 12 - The possibility of extensive genetic testing...Ch. 12 - SCIENTIFIC THINKING Scientists investigate...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Choose the one answer that fits best. Which statement regarding PCR is NOT correct (videos)? a. PCR requires a copy of RNA that serves as a template b. Taq polymerase adds nucleotides to the primers and creates a complementary strand of DNA c. Annealing requires cooler temperatures than denaturation d. Repeated cycles of denaturation, annealing and extending DNA strands creates many identical copies of DNA e. PCR is a quick way of using minute quantities of DNA to create millions of copiesarrow_forwardChoose the combination of answers that most accurately completes the statement.For which of the following would a nucleic acid probe not be used? a. locating a gene on a chromosome b. developing a Southern blot c. identifying a microorganism d. constructing a recombinant plasmidarrow_forwardBrenda is a junior student in the biomedical program at her school. She is starting the PCR genetic testing lab activity. She is about to obtain her DNA sample but doesn’t want like the taste of NaCl solution. Her friend, Mark, let her use some of his DNA. What laboratory tule did the students break? A. Obtaining and handling DNA sample without wearing googles or gloves B. Improper use of human DNA samples C. Violating Patient Confidentiality D. Disposing of bio hazardous material in a regular trasharrow_forward
- Which of the following techniques separate molecules by size using electric charge? a. Group of answer choices b. Gel electrophoresis c. PCR d. restriction enzymes gel filteringarrow_forwardYou have been performing a PCR reaction but your results aren't the greatest. Your Supervisor has told you that you should increase the concentration of Magnesium. What affect will this have on the reaction? a. The annealing temperature will not be affected but the enzyme activity will be affected. b. The annealing temperature will decrease. c. The denaturation temparture will have to be increased in the PCR protocol. d. The denaturation temparture will have to be decreased in the PCR protocol. e. The Annealing temperature will increase.arrow_forwardAnswer Part d,e,farrow_forward
- In sequential order, what are the three steps of PCR? A. Denature DNA, Anneal Primers, Extend DNA B. Denature DNA, Extend DNA, Anneal Primers C. Anneal Primers, Extend DNA, Denature DNA D. Extend DNA, Anneal Primers, Denature DNAarrow_forwardMatch the following terms with their definitions and label each component of the PCR mixture in the diagram (use the letters A-D):I. DNA polymeraseII. PrimersIII. NucleotidesIV. Genomic DNA template A. DNA that contains the target sequence that will be replicated using PCR.B. An enzyme that copies the DNA sequence.C. A mixture of 4 nucleotides (A,G,C, and T) that will be polymerized into the replicated DNA sequence.D. A short DNA sequence that allows the enzyme to bind and initiate polymerization.arrow_forwardWhich of the following is involved in recombinant DNA technology? Explain. a. DNA polymerase b. DNA probes c. Restrition enzymes d. Reverse transcriptasearrow_forward
- Provide the role of the following in the crude DNA extraction:a. salt b. cold alcohol c. cold distilled water d. blending or blender methode. liquid detergentarrow_forwardUse the plasmid to answer the following questions a) Digest the plasmid with EcoRI only and then both EcoRI and BamHi together. Fill in the table to show the fragment sizes produced.arrow_forwardThe following question is related to Restriction Enzymes and RFLP. Using EcoRl, how many cuts were performed on DNA sequence A? A.) 2 B.) 3 C.) 4arrow_forward
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