Campbell Biology: Concepts & Connections (8th Edition)
8th Edition
ISBN: 9780321885326
Author: Jane B. Reece, Martha R. Taylor, Eric J. Simon, Jean L. Dickey, Kelly A. Hogan
Publisher: PEARSON
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Textbook Question
Chapter 12, Problem 8TYK
Recombinant DNA techniques are used to custom-build bacteria for two main purposes: to obtain multiple copies of certain genes and to obtain useful proteins produced by certain genes. Give an example of each of these applications in medicine and agriculture.
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How can site-specific recombination be used in recombinant DNA technology? Explain with an example
describe how the bacteria should have the ability to be recovered back from the infected host for the purpose of experimentation including sequencing ,vitro culturing and polymerase chain reaction amplification.
In bacterial transformation, the purpose of having antibiotic within an agar plate is to:
Select one:
confirm which plasmids been have successfully ligated with a gene of interest.
isolate bacteria which have been successfully transformed with the plasmid.
indicate which plasmids were successfully digested by the endonuclease.
act as a substrate which will be cleaved and produce a blue product when ligation is unsuccessful.
show which plasmids contain the lacZ gene.
Chapter 12 Solutions
Campbell Biology: Concepts & Connections (8th Edition)
Ch. 12 - Imagine you have found a small quantity of DNA....Ch. 12 - Which of the following would be considered a...Ch. 12 - The DNA profiles used as evidence in a murder...Ch. 12 - A paleontologist has recovered a tiny bit of...Ch. 12 - How many genes are there in a human sperm cell? a....Ch. 12 - When a typical restriction enzyme cuts a DNA...Ch. 12 - Why does DNA profiling rely on comparing specific...Ch. 12 - Recombinant DNA techniques are used to...Ch. 12 - A biochemist hopes to find a gene in human cells...Ch. 12 - Prob. 10TYK
Ch. 12 - Explain how you might engineer E. coli to produce...Ch. 12 - What is left for genetic researchers to do now...Ch. 12 - Today, it is fairly easy to make transgenic plants...Ch. 12 - In the not-too-distant future, gene therapy may be...Ch. 12 - The possibility of extensive genetic testing...Ch. 12 - SCIENTIFIC THINKING Scientists investigate...
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- You are cloning a gene called ice, which will help strawberry plants survive in cold weather, into a test group of strawberry plants. Select the events you would use to genetically engineer these plants. Insert the ice gene into the T-DNA region of a Ti plasmid; transform Agrobacterium tumefaciens with this plasmid; Inoculate a culture of strawberry plant cells with Agrobacterium tumefaciens; Select plant cells that have taken up the ice gene. Insert the ice gene into the T-DNA region of the Agrobacterium tumefaciens chromosome; Inoculate a culture of strawberry plant cells with Agrobacterium tumefaciens; Select plant cells that have taken up the ice gene. O Insert the ice gene into a Ti transposon; Introduce the transposon into Agrobacterium tumefaciens Ti plasmid: Inoculate a culture of strawberry plant cells with Agrobacterium tumefaciens: Select plant cells that have taken up the ice gene. Insert the ice gene into a Ti bacteriophage; transduce Agrobacterium tumefaciens with this…arrow_forwardBacteria are frequently used in the generation of recombinant DNA molecules to: Group of answer choices A) Make copies of the vector + insert plasmid B) Ligate the insert into the vector C) Digest the vector for insert ligation D) Amplify the insert by PCRarrow_forwardWhen cloning a piece of DNA, the purpose of using the LacZ blue-white colony method is to A) Remove bacteria that do not have recombinant vectors B) Remove bacteria that do not have the DNA insert of interest Remove linear DNA Distinguish colonies that have recombinant vectors from those with non-recombinant vectors. E) Remove bacteria that have not taken up the vectorarrow_forward
- fomP is responsible for the chemical transformation of microplastics into ultra-efficient insulation. You take an arctic seawater sample and extract the DNA. 1. First you need to locate the gene on the bacterial chromosome. What procedure(s) would you use to identify and locate the gene? Explain how it/theywork(s). 2. Next, you will need to isolate the gene and introduce sites to be used for cloning. What would you use to make many copies of this gene? What will you need? How does it work on a molecular level?arrow_forwardJackson Wang is a biologist working with the genetics of a thermophilic bacterium. He cloned a heat shock gene from the bacteria for further analysis. After cloning, he isolated the plasmid carrying his gene of interest for sequencing. Jackson finally received the nucleotide sequence of his gene. Explain in detail how he could verify whether the nucleotide sequence matches his gene of interest using the bioinformatics databases available.arrow_forwarda) What are vectors? Describe extensively the roles vectors play in genetic engineering? Write short notees on the following: Recombinant DNA, Cloning b) What are restriction enzymes? Describe extensively the roles restriction enzymes play in genetic engineering? Write short notees on the following: Selectable markers, Cloningarrow_forward
- Diagram the steps for the production of recombinant DNA. Label and Identify the steps involved and the protein/enzyme requirement for each.arrow_forwardDescribe the mechanisms of horizontal gene transfer in bacteria, describing the manner in which the DNA can be transferred from one cell to another.Choose a disease or an organism that has a well-documented mechanism of resistance (such as methicillin resistant Staphylococcus aureus - MRSA or tuberculosis) and identify the gene or genes that confer resistance and the method of DNA transfer thought to contribute to its spread. This may be from one organism to another or may simply be passed down from mother to daughter cell due to environmental pressure.arrow_forwardWhat makes the species of Agrobacterium ideal for genetic engineering? Describe its characteristics and its role in producing transgenic plants.arrow_forward
- Explain how biotechnology and bioinformatics are used to identify microorganisms Explain why biotechnology has replaced many of the traditional diagnostic tools in microbiology Explain why a PCR reaction needs a source of nucleotides in the form of ATP, GTP, CTP and TTP. Explain what primers are and describe their use in PCR reactions Given the results from a PCR/Gel electrophoresis or a DNA sequence; determine the identity of a bacterium or virus...arrow_forwardPCR has many useful applications. However, an incorrect application of PCR is: a) Amplification of any gene of interest from genomic DNA b) Screening various foods for genes that are typical in genetically modified organisms c) Identification of microbial DNA in a sample (viral or bacterial) d) Amplification of any protein of interestarrow_forwardDEFINE THE FOLLOWING: 1) restriction enzyme 2) plasmid 3) recombinant DNAarrow_forward
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genetic recombination strategies of bacteria CONJUGATION, TRANSDUCTION AND TRANSFORMATION; Author: Scientist Cindy;https://www.youtube.com/watch?v=_Va8FZJEl9A;License: Standard youtube license