Campbell Biology: Concepts & Connections (8th Edition)
Campbell Biology: Concepts & Connections (8th Edition)
8th Edition
ISBN: 9780321885326
Author: Jane B. Reece, Martha R. Taylor, Eric J. Simon, Jean L. Dickey, Kelly A. Hogan
Publisher: PEARSON
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Chapter 12, Problem 6TYK

When a typical restriction enzyme cuts a DNA molecule, the cuts are uneven, giving the DNA fragments single-stranded ends. These ends are useful in recombinant DNA work because

a. they enable a cell to recognize fragments produced by the enzyme.

b. they serve as starting points for DNA replication.

c. the fragments will bond to other fragments with complementary ends.

d. they enable researchers to use the fragments as molecular probes.

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Which of the following describes an advantage of using a recombinant plasmid for DNA cloning over PCR? A. PCR is more likely to have errors introduced in the copying process. B. Recombinant DNA plasmids are able to create large amounts of copies more quickly than PCR. C. PCR can only be conducted in eukaryotic cells. D. PCR requires prior knowledge of the sequence in question, while a recombinant plasmid does not.
Which of the following statements about DNA probes are false? a.  Probes can be labeled with a fluorescent dye to facilitate detection. b. Probes can be labeled with radioactive phosphorus to facilitate detection. c. Probes are typically composed of double stranded pieces of DNA. d. Probes are typically composed of single stranded pieces of DNA.
Some restriction enzymes produce DNA fragments with overhanging stretches called sticky ends on each strand.  Sticky ends are useful in making recombinant DNA because Select one: a.  Sticky ends contain the exact same nucleotides that allows fragments to splice together. b.  Sticky ends contain nucleotides with complementary bases that allows fragments to splice together. c. Sticky ends contain the exact same nucleotides that can form hydrogen bonds. d. Sticky ends contain nucleotides with complementary bases that can form hydrogen bonds.
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