Campbell Biology: Concepts & Connections (8th Edition)
8th Edition
ISBN: 9780321885326
Author: Jane B. Reece, Martha R. Taylor, Eric J. Simon, Jean L. Dickey, Kelly A. Hogan
Publisher: PEARSON
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Chapter 12, Problem 6TYK
When a typical restriction enzyme cuts a DNA molecule, the cuts are uneven, giving the DNA fragments single-stranded ends. These ends are useful in recombinant DNA work because
a. they enable a cell to recognize fragments produced by the enzyme.
b. they serve as starting points for
c. the fragments will bond to other fragments with complementary ends.
d. they enable researchers to use the fragments as molecular probes.
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Students have asked these similar questions
Which of the following describes an advantage of using a recombinant plasmid for DNA cloning over PCR?
A. PCR is more likely to have errors introduced in the copying process.
B. Recombinant DNA plasmids are able to create large amounts of copies more quickly than PCR.
C. PCR can only be conducted in eukaryotic cells.
D. PCR requires prior knowledge of the sequence in question, while a recombinant plasmid does not.
Which of the following statements about DNA probes are false?
a. Probes can be labeled with a fluorescent dye to facilitate detection.
b. Probes can be labeled with radioactive phosphorus to facilitate detection.
c. Probes are typically composed of double stranded pieces of DNA.
d. Probes are typically composed of single stranded pieces of DNA.
Some restriction enzymes produce DNA fragments with overhanging stretches called sticky ends on each strand. Sticky ends are useful in making recombinant DNA because
Select one:
a. Sticky ends contain the exact same nucleotides that allows fragments to splice together.
b. Sticky ends contain nucleotides with complementary bases that allows fragments to splice together.
c. Sticky ends contain the exact same nucleotides that can form hydrogen bonds.
d. Sticky ends contain nucleotides with complementary bases that can form hydrogen bonds.
Chapter 12 Solutions
Campbell Biology: Concepts & Connections (8th Edition)
Ch. 12 - Imagine you have found a small quantity of DNA....Ch. 12 - Which of the following would be considered a...Ch. 12 - The DNA profiles used as evidence in a murder...Ch. 12 - A paleontologist has recovered a tiny bit of...Ch. 12 - How many genes are there in a human sperm cell? a....Ch. 12 - When a typical restriction enzyme cuts a DNA...Ch. 12 - Why does DNA profiling rely on comparing specific...Ch. 12 - Recombinant DNA techniques are used to...Ch. 12 - A biochemist hopes to find a gene in human cells...Ch. 12 - Prob. 10TYK
Ch. 12 - Explain how you might engineer E. coli to produce...Ch. 12 - What is left for genetic researchers to do now...Ch. 12 - Today, it is fairly easy to make transgenic plants...Ch. 12 - In the not-too-distant future, gene therapy may be...Ch. 12 - The possibility of extensive genetic testing...Ch. 12 - SCIENTIFIC THINKING Scientists investigate...
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- The function of a restriction enzyme is to a. prevent the movement of DNA outside the nucleus b. separate the DNA double helix c. cut the nucleotide sequence at a specific location in DNA d. proofread DNA for accidental damages and corrects these errorsarrow_forwardPlace the steps of sanger sequencing in order.A. A fluorescent laser excites the fragments and records the wavelength consistent with a single nucleotide. B. ddNTPs bind and stop chain extension.C. DNA fragments are separated by size through a capillary tube. D. DNA polymerase copies the target region of template DNA.E. The final nucleotide of each fragment is labeled with a fluorescent tag.arrow_forwardExplain how electrophoresis separates DNA strands. a. How is a DNA fingerprinting test interpreted? b. Define plasmid and how plasmids can change a bacteria’s activity. c. How do we digest/cleave plasmids? Explain the role of a restriction enzyme. d. Define sticky end and blunt end and which one is useful in molecular biology.arrow_forward
- Certain restriction endonucleases produce cohesive (sticky) ends. This means that they: a. stick tightly to the ends of the DNA they have cut. b. cut both DNA strands at the same base pair. c. make a staggered double-strand cut, leaving ends with a few nucleotides of single-stranded DNA protruding. d. cut in regions of high GC content, leaving ends that can form more hydrogen bonds than ends of high AT content. e. cut in regions of high AT content, leaving ends that can form more hydrogen bonds than ends of high GC content.arrow_forwardA researcher is performing PCR to amplify a sample of DNA. Unfortunately, he forgot to add the DNA primer prior to starting the experiment. Which of the following results is he most likely to observe? a. The reaction will work, but at a significantly slower rate. b. The reaction will work, but the product will contain many undesired mutations. c. The reaction will work, but amplify a region that was not his target. d. The reaction will be completely unsuccessfuarrow_forwardWhich of the following describes sequencing by synthesis? A. Large sections of DNA are inserted into a plasmid. B. DNA sequence is recorded as the complementary strand is built. C. Many copies of the DNA are made, each terminating at a specific nucleotide. D. A DNA strand is forced through a pore and its sequence synthesized as it passes through.arrow_forward
- Which of the following is NOT true of DNA polymerase? a. DNA polymerase builds the new strand from 5' to 3'. b. DNA polymerase can start new DNA strands independently. c. DNA polymerase uses a "guess and check" method to add nucleotides. d. DNA polymerase uses dATP as a nucleotide. e. DNA polymerase reads the template from 3' to 5'.arrow_forwardA linear piece of DNA is cut by two restriction enzymes. One restriction enzyme will cut the target DNA once. The other restriction enzyme will cut the target DNA twice. How many fragments of DNA should result from this reaction? a. 3 fragments of DNA b. 4 fragments of DNA c. 5 fragments of DNA d. 6 fragments of DNA e. None of the abovearrow_forwardThe restriction endonucleases used in recombinant DNA work: a. are synthesized by bacteria b. recognize sequences 14-16 bp long c. cut the DNA outside the recognition sequence d. all the above are truearrow_forward
- DNA fragments that are 500 bp, 1000 bp, and 2000 bp in length are separated by gel electrophoresis. Which fragment will migrate farthest in the gel? a. The 2000-bp fragment b. The 1000-bp fragment c. The 500-bp fragment d. All will migrate equal distances.arrow_forwardArrange the following steps in the sequence they would happen in a DNA cloning experiment. a. sealing DNA fragments into vectors with DNA ligase; b. utilizing a probe to detect a clone in the library; c. sequencing the clone's DNA; d. creating a DNA library of clones; e. cutting genomic DNA with restriction enzymes. A. e,a,d,b,c B. a,d,b,c,e C. c,b,e,a,d D. e,d,a,c,barrow_forwardPCR is used to: Select one: a. Randomly break large pieces of DNA or plasmids into smaller fragments. O b. Determine if a particular piece of DNA contains a certain gene. Oc. Amplify a specific segment of DNA. d. Separate DNA fragments based on size. e. Isolate specific pieces of DNA. ess Jump to...arrow_forward
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