Below are some real data I collected doing a glutamate uptake experiment. I plated astrocytes in a dish and applied glutamate spiked with a radioactive tracer. Because I was measuring glutamate transport (and not binding of glutamate to a receptor), I measured velocity instead of binding: pmoles of glutamate transported per minute, normalized to the amount of protein (mg) in the cells. Use the figure to answer the following questions: Velocity/Concentration (pmol/mg/min/uM) 300 250 200 150 100 50 Glutamate Uptake Pharmacodynamics y = -0.0158x + 119.79 y = -0.0169x + 271.33 0 0 2000 4000 6000 8000 10000 12000 14000 16000 18000 Velocity (pmol/mg/min) Control Condition Experimental Condition

Below are some real data I collected doing a glutamate uptake experiment. I plated astrocytes in a dish and applied glutamate spiked with a radioactive tracer. Because I was measuring glutamate transport (and not binding of glutamate to a receptor), I measured velocity instead of binding: pmoles of glutamate transported per minute, normalized to the amount of protein (mg) in the cells. Use the figure to answer the following questions: Velocity/Concentration (pmol/mg/min/uM) 300 250 200 150 100 50 Glutamate Uptake Pharmacodynamics y = -0.0158x + 119.79 y = -0.0169x + 271.33 0 0 2000 4000 6000 8000 10000 12000 14000 16000 18000 Velocity (pmol/mg/min) Control Condition Experimental Condition

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter10: Reconstitution Of Powdered Drugs

Section: Chapter Questions

Problem 25SST

See similar textbooks

Similar questions

Now draw a rough sketch of what the control data might look like if in addition to the specific binding, there was also a considerable amount of nonspecific binding (again using a normal dose/response curve) (do % total bound ligand vs concentration)
One of the important uses of the Nernst equation is in describing the flow of ions across plasma membranes. Ions move under the influence of two forces: the concentration gradient (given in electrical units by the Nernst equation) and the electrical gradient (given by the membrane voltage). This is summarized by Ohms law: Ix=Gx(VmEx) which describes the movement of ion x across the membrane. I is the current in amperes (A); G is the conductance, a measure of the permeability of x, in Siemens (S), which is I/V;Vm is the membrane voltage; and Ex is the equilibrium potential of ion x. Not only does this equation tell how large the current is, but it also tells what direction the current is flowing. By convention, a negative value of the current represents either a positive ion entering the cell or a negative ion leaving the cell. The opposite is true of a positive value of the current. a. Using the following information, calculate the magnitude of Na [ Na+ ]0=145mM,[ Na+ ]i=15mM,Gna+=1nS,Vm=70mV b. Is Na+ entering or leaving the cell? c. Is Na+ moving with or against the concentration gradient? Is it moving with or against the electrical gradient?
Describe the contribution of each of the following to establishing and maintaining membrane potential: (a) the Na+K+ pump, (b) passive movement of K+ across the membrane, (c) passive movement of Na+ across the membrane, and (d) the large intracellular anions.
The rate at which the Na+K+ pump operates is not constant but is controlled by a combined effect of changes in ICF Na+ concentration and ECF K+ concentration. Do you think the changes in both ICF Na+ and ECF K+ concentration following a series of action potentials in a neuron would accelerate, slow down, or have no effect on the Na+K+ pumps in this cell?
Outline the three general means by which binding of a water soluble extracellular chemical messenger to its matching surface membrane receptor brings about the desired intracellular response.
Second-messenger systems ultimately bring about the desired cell response by inducing a change in the shape and function of particular designated intracellular proteins. (True or false?)
Using the Nernst equation, calculate the equilibrium potential for Ca2 and for C1 from the following sets of data: a. Given [ Ca2+ ]0=1mM,[ Ca2+ ]i=100nM, find Eca2+ b. Given [ Cl- ]0=110mM,[ Cl- ]i=100mM, find Ecl
Using the Goldman-Hodgkin-Katz equation, determine what happens to the resting membrane potential if the ECF K+ concentration doubles to 10 mM.
How is an extracellular signal converted to an intracellular signal in signal transduction? Give a specific example.
A common membrane-bound intermediary between the receptor and the effector protein within the plasma membrane is the __________________.
Using the answer code on the right, indicate which membrane component is responsible for the function in question: 1. channel formation____________________________________ (a) lipid bilayer 2. barrier to passage of water- __________________ (b) proteins soluble substances ____________________________________ (c) carbohydrates 3. receptor sites 4. membrane fluidity 5. recognition of self 6. membrane-bound enzymes 7. structural boundary 8. carriers
Answer the following questions regarding conduction of action potentials using the velocities given on p. 100: a. How long would it take for an action potential to travel 0.6 m along the axon of an unmyelinated neuron of the digestive tract? b. How long would it take for an action potential to travel the same distance along the axon of a large myelinated neuron innervating a skeletal muscle? c. Suppose there were two synapses in a 0.6 m nerve tract and the delay at each synapse is 1 msec. How long would it take an action potential and chemical signal to travel the 0.6 m now, for both the myelinated and unmyelinated neurons? d. What if there were five synapses?