a) When you added the primary antibody to the wells, what happened if the sample contained the antigen? What if it did not contain the antigen? b) When you added the secondary antibody to the wells, what happened if the sample contained the antigen? What if it did not contain the antigen? c) If your sample gave a negative result for the antigen does this guarantee the antigen is not present? What reasons could there be for a negative result when the antigen is actually present?

a) When you added the primary antibody to the wells, what happened if the sample contained the antigen? What if it did not contain the antigen? b) When you added the secondary antibody to the wells, what happened if the sample contained the antigen? What if it did not contain the antigen? c) If your sample gave a negative result for the antigen does this guarantee the antigen is not present? What reasons could there be for a negative result when the antigen is actually present?

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter4: Basic Immunology And Immunohematology

Section4.7: Rh Typing

Problem 3RQ

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Similar questions

a) When you added the primary antibody to the wells, what happened if the sample contained the antigen? What if it did not contain the antigen? b) When you added the secondary antibody to the wells, what happened if the sample contained the antigen? What if it did not contain the antigen?
If you were using the ELISA to look for the presence of antibodies and the sample gave a negative result, does this mean that there were no antibodies present? Explain.
a) Which sample(s) contained the antigen? b)The samples you added to the microplate strip contain many proteins and may or may not contain the antigen. What happened to the proteins in the plastic well if the sample contained the antigen? If it did not contain the antigen? c) Why did you need to wash the wells after every step?
a) What does ELISA stand for and what is the purpose of this type of test. b) Which region of the antigen is at the tips, and what do the variable regions do during an infection to an antigen? c) What are the four components of the ELISA test and what do they do?
In a direct ELISA, which of the following does the patient provide? A.) Substrate B.) Enzymes C.) Antibodies D.) Antigens
A technologist failed to notice that the centrifuge had not properly centrifuged the test tubes prepared for antibody identification. The time of centrifugation was 15 seconds instead of 30 seconds. What would be the potential error in the interpretation of this test? can please any one provide me ans?
When the ELISA test was conducted on an immune-suppressed person, he tested positive for a pathogen. (a) Identify the disease the patient is suffering from. (b) Name the causative entity. (c) Mention the cells of the body that are attacked by the pathogen.
If the patient's mother had previously vaccinated her son against chickenpox, what form of immunity would he have had against the virus? A) Active natural immunity B) Passive natural immunity C) Active artificial immunity D) Passive artificial immunity
Choose all that are correct about indirect ELISA. A) An antigen can bind the substrate B) A secondary antibody can bind the antigen C) A secondary antibody can bind a primary antibody D) A primary antibody can bind an antigen
To diagnosis, we used a procedure known as a "sandwich ELISA" (Enzyme-Linked Immunosorbent Assay). 1.In the "ELISA sandwich," what is the "meat"? 2.Both patients A and B had positive results. Why are these two patients' outcomes so different? 3.What is one potential cause of an ELISA false negative result? What is one potential cause of an ELISA false positive result? What is another example of how an ELISA assay should be used?
All of the following are used in a direct ELISA, except: A) Primary enzyme linked antibody B) Substrate C) Secondary enzyme linked antibody D) Primary antibody
When a nurse takes your blood, why must the collection tube be heparinized? A) To prevent the blood from agglutinating. B) To keep the blood fresh so the components don't break down before the diagnostic tests can be run. C) To prevent the blood from clotting after collection. D) To prepare the blood for a specific diagnostic test that is going to be performed.