Brock Biology of Microorganisms (14th Edition)
14th Edition
ISBN: 9780321897398
Author: Michael T. Madigan, John M. Martinko, Kelly S. Bender, Daniel H. Buckley, David A. Stahl, Thomas Brock
Publisher: PEARSON
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Textbook Question
Chapter 12, Problem 3AQ
Imagine that you have been given several bacterial strains from various countries around the world and that all the strains are thought to cause the same gastrointestinal disease and to be genetically identical. Upon carrying out a DNA fingerprint analysis of the strains, you find that four different strain types are present. What methods could you use to test whether the different strains are actually members of the same species?
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You are given two samples of DNA, one from Clostridium perfringens and the other from
Mycobacterium tuberculosis. Unfortunately, the labels on the tubes of DNA were accidentally
removed, and you do not know which DNA sample belongs to which organism. As you were
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well. The %G + %C values for each of the organisms is known (C. perfringens, 27%; M.
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determine the source of the DNA for your two samples?
In your own words
You culture bacteria from the soil at a toxic waste dump on an agar plate and pick a single bacterium from which you prepare DNA. The
isolated organism is a new, previously uncharacterized species. You perform PCR amplification of this DNA using primers to amplify a
short segment of ribosomal DNA. When you use BLAST to find similar sequences, you find the following results.
Organism
E. coli
Streptococcus
Burkhoderia
Microbacterium oxydans
DNA sequence identity. % sequence E-value
coverage
e-10
E. coli
84%
88%
98%
100%
40%
40%
40%
80%
e-12
e-15
e-30
Based on the data in the table, which species is most closely related to your toxic waste dump sample?
Microbacterium oxydans
Streptococcus
Burkhoderia
Chapter 12 Solutions
Brock Biology of Microorganisms (14th Edition)
Ch. 12.1 - What characteristics would have made the surface...Ch. 12.1 - How do we know when oceans were first present on...Ch. 12.1 - What lines of reasoning support the hypothesis...Ch. 12.2 - Why is the origin of cyanobacteria considered a...Ch. 12.2 - What caused the development of banded iron...Ch. 12.2 - What lines of evidence indicate that microbial...Ch. 12.3 - What evidence supports the idea that the...Ch. 12.3 - Prob. 2MQCh. 12.3 - In what ways are modern eukaryotes a combination...Ch. 12.4 - Prob. 1MQ
Ch. 12.4 - Prob. 2MQCh. 12.4 - Prob. 3MQCh. 12.5 - How are DNA sequences obtained for phylogenetic...Ch. 12.5 - What does a phylogenetic tree depict?Ch. 12.5 - Prob. 3MQCh. 12.6 - What are the different processes that give rise to...Ch. 12.6 - What is the difference between selection and...Ch. 12.6 - In the experiment of Figure 13.12, why did the...Ch. 12.7 - What is the difference between the core and pan...Ch. 12.7 - What kind of recombination might have the greatest...Ch. 12.7 - What effects do deletions have on the evolution of...Ch. 12.8 - What is the difference between taxonomy and...Ch. 12.8 - What are some key criteria from the phylogenetic...Ch. 12.8 - How many species of Bacteria and Archaea have been...Ch. 12.9 - What class of genes is used in MLST analyses?Ch. 12.9 - How is ribotyping different from rep-PCR?Ch. 12.9 - What is FAME analysis?Ch. 12.10 - What roles do culture collections play in...Ch. 12.10 - What is the IJSEM and what taxonomic function does...Ch. 12.10 - Why might viable cell cultures be of more use in...Ch. 12 - Prob. 1RQCh. 12 - Prob. 2RQCh. 12 - Prob. 3RQCh. 12 - What is the endosymbiotic hypothesis for the...Ch. 12 - Prob. 5RQCh. 12 - REVIEW QUESTIONS
6. What major physiological and...Ch. 12 - Prob. 7RQCh. 12 - Prob. 8RQCh. 12 - Prob. 9RQCh. 12 - What is the difference between a gene tree and an...Ch. 12 - Prob. 11RQCh. 12 - Prob. 12RQCh. 12 - What is fitness? To what degree does fitness...Ch. 12 - What are some processes that influence the content...Ch. 12 - Prob. 15RQCh. 12 - What is the "species problem" and why is the...Ch. 12 - Prob. 17RQCh. 12 - Prob. 18RQCh. 12 - Prob. 19RQCh. 12 - Prob. 20RQCh. 12 - Prob. 21RQCh. 12 - Compare and contrast the physical and chemical...Ch. 12 - For the following sequences, construct the...Ch. 12 - Imagine that you have been given several bacterial...Ch. 12 - Imagine that you have discovered a new form of...
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