L 1 2 3 Locus A Locus B L = Standard marker 1 = Suspect 1 2 = Suspect 2 3 = DNA from crime scene

Q: You have a DNA sequence of 1800 bp. To characterize it, you decide to make a restriction map.…

A: Restriction mapping is a process that helps to determine the location of the restriction site. The…

Q: The figure below represents the size of various SSRS that are used for forensic analysis. The bars…

A: vWA produce 200bp in PCR . All others produce less or more than 200 bp in PCR.  Here SSR means is…

Q: If a sample of double-stranded DNA gave the following results, what is the concentration (ng/uL) of…

A: The absorption value of 1 at A260 corresponds to 50 ug/ml So, the value of 1.637 at A260 corresponds…

Q: The following figure shows the FBI-style analysis of the genomic DNA of 10 people (1-10), and also…

A: Chromosomes are carrier of deoxyribonucleic acid (DNA). DNA is the genetic material. Simple sequence…

Q: do you need to put the reagents in the reaction tubes into the tubes in a specific order for DNA…

A: DNA fingerprint analysis consists of the process of identification of individuals based on their DNA…

Q: An AGE run was set at 100V for 30 min, where 3 ul of the ladder (100 ng/ul) was loaded into the gel,…

A: Lane 1 bottom band has below 500 basepairs. Lane 1 top band has 5000bp. Lane 2 band 1000bp.

Q: You have 15 tubes, each containing 5 ul of DNA (each tube has a different DNA to cut). You want to…

A: A reaction mixture is a combination of 2 or more reactants in definite proportions to carry out a…

Q: What will happen to the concentration and the A260/280 ratio of the DNA sample in the following…

A: Biotechnology is the use of our understanding of biological processes to develop useful applications…

Q: Identify what is false for the following statement: In order to use PCR to detect genes, primers…

A: Kary B. Mullis invented the Polymerase Chain Reaction (PCR) in 1983 as a method for amplification of…

Q: What is the sequence of the sample DNA submitted for sequencing given the gel electrophoresis…

A: Sanger sequencing method is chain terminating method.

Q: You have run a gel with 5 uL of amplified DNA in addition to 1 uL of 6X loading dye. Comparing the…

A: Agarose Gel Electrophoresis (AGE), is an biochemistry technique which is used to estimate DNA…

Q: Which female could be the mother of the child and why? Which male could be the father of the child…

A: There are several ways to identify the mother of a child from VNTR loci. One way is to look at the…

Q: Someone broke into the bookstore and stole thousands of dollars’ worth of textbooks. Some hairs were…

A: Most likely the culprit is suspect 1. This is so as the banding pattern of suspect 1 is matching…

Q: Using the designed forward and reverse primer from Question 23-30, you performed PCR to amplify the…

A: The first step in designing a PCR primer to amplify the CO1 gene of S. tawilis and S. malengi is to…

Q: Are any of the loci on the X chromosome? If so, identify this (these) locus (loci) by color.

A: The term SSR stands for simple sequence repeat that is a microsatellite VNTR (variable number tandem…

Q: The gel below shows results for the bitter tasting SNP analysis. Analyze the results and annotate…

A: PCR or polymerase chain reaction is a technique that is used to amplify the desired segment of DNA…

Q: What is the formula used to calculate the number of DNA molecules which will be created for a given…

A: PCR or polymerase chain reaction is a technique of DNA amplification. Here a small amount of DNA is…

Q: Complete this Master Mix table for 8 DNA samples, a positive control, negative control, and an extra…

A: Volume per tube (uL) number of reactions volume in master mix (uL) PCR buffer 1.2 11 13.2 dNTP…

Q: Determine which primer pair is the best choice. • Explain why the other primers are not good…

A: A brief DNA or RNA sequence serves as the template and controls the production of a complementary…

Q: A 100ml dsDNA solution prepared via 1:20 dilution was taken for spectrometry analysis. The A260 and…

A: The objective of the question is to calculate the total DNA yield of the solution in micrograms. The…

Q: 1) you have a locus in your PCR reaction that is a DNA squence is on the fifth chromosome, is a…

A: Gel electrophoresis is a common laboratory method used for the separation of DNA, RNA, or proteins…

Q: Who stole Mr. Marshall's blue stapler? A suspect was seen fleeing from Peck with Mr. Marshall's blue…

A: Examining genetic material taken from a living thing can provide meaningful insight. By making many…

Q: In STR DNA typing, a typical DNA pattern shows (two, three) bands.

A: In STR DNA typing, a typical DNA pattern shows (two,) bands.

Q: The following gel shows the results of a crime scene investigation. Lane 1 shows a DNA sample that…

A: Gel electrophoresis:- it is a technique used for the separation of Nucleic acids i.e. DNA and RNA as…

Q: In lane 1, a size standard was loaded, which contained a mix a DNA fragments known to be 1000 bp,…

A: Gel electrophoresis is a gel technique that uses an applied electrical field to separate nucleic…

Q: In lane 1, a size standard was loaded, which contained a mix a DNA fragments known to be 1000 bp,…

A: Introduction Comparison between the location and size of the standard and human samples dictates the…

Crime Scene Investigation

A crime scene refers to a place where a crime has occurred. The purpose of crime scene investigation (CSI) is to analyze the complete scene and catch the culprit based on the pieces of evidence collected from the place of crime. The people involved in this process are referred to as crime scene investigators.