Chapter 11, Problem 13P

Interpretation Introduction

(a) Interpretation:

Calculation of the fraction of monomers that exist in the heterodimer complex of the total concentration of A+B inside the cell is 5 μm and K_d is 1 μm.

Concept introduction:

The protein has a complex structure in which two monomers in their dimer form make the complex for effective function. The degree of saturation is termed as the fraction of monomer that has bonded with ligand in a protein complex.

Interpretation Introduction

(b) Interpretation:

Calculation of the fractions of monomers if the cell is now disrupted in the buffer, the cell contents are diluted 5-fold.

Concept introduction:

The protein has a complex structure in which two monomers in their dimer form make the complex for effective function. The degree of saturation is termed as the fraction of monomer that has bonded with ligand in a protein complex.

Interpretation Introduction

(c) Interpretation:

The methods of detection of multiprotein complexes in cell-free homogenatesand discuss the methods of detection of the proteins.

Concept introduction:

The protein has a complex structure in which two monomers in their dimer form make the complex for effective function. The degree of saturation is termed as the fraction of monomer that has bonded with ligand in a protein complex.