Microbiology: A Systems Approach
4th Edition
ISBN: 9780073402437
Author: Marjorie Kelly Cowan Professor
Publisher: McGraw-Hill Education
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Question
Chapter 10.2, Problem 5AYP
Summary Introduction
To describe:
The way in which recombinant DNA is created and also discuss its role in gene cloning.
Concept introduction
Any process by which scientists can manipulate the genetic material of the organism in the laboratory by deletion, addition or substitution of DNA is known as genetic engineering.
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Explain the role of reverse transcriptase in cloning.
Describe the cloning of DNA.
List all steps involved in a cloning workflow. Name and link the appropriate enzymes to the steps at which they are used.
Chapter 10 Solutions
Microbiology: A Systems Approach
Ch. 10.1 - Provide examples of practical applications of...Ch. 10.2 - Prob. 2AYPCh. 10.2 - Describe how gel electrophoresis is used to...Ch. 10.2 - Prob. 4AYPCh. 10.2 - Prob. 5AYPCh. 10.3 - Prob. 6AYPCh. 10.3 - List examples of genetically modified bacteria,...Ch. 10.4 - Prob. 8AYPCh. 10.4 - Prob. 9AYPCh. 10.5 - Prob. 2CF
Ch. 10.5 - Outline in general terms the process of DNA...Ch. 10.5 - Prob. 11AYPCh. 10.5 - Prob. 12AYPCh. 10.5 - Prob. 13AYPCh. 10.6 - Prob. 14AYPCh. 10 - Prob. 1CFCh. 10 - Which of the following is/are not essential to...Ch. 10 - Prob. 2MCQCh. 10 - The function of ligase is to a. rejoin segments of...Ch. 10 - The creation of biological molecules entirely from...Ch. 10 - Which of the following sequences, when combined...Ch. 10 - A region of DNA in a plasmid that is recognized by...Ch. 10 - Prob. 7MCQCh. 10 - Which of the following is a primary participant in...Ch. 10 - Single nucleotide polymorphisms are found in a....Ch. 10 - Microarrays are used to monitor a. the rate of DNA...Ch. 10 - Prob. 11TFCh. 10 - A nucleic acid probe can be used to identify...Ch. 10 - Prob. 13TFCh. 10 - In order to detect recombinant cells, plasmids...Ch. 10 - Plasmids are the only vectors currently available...Ch. 10 - You are a public health official trying to...Ch. 10 - a.Construct a strand of complementary DNA (cDNA)...Ch. 10 - Prob. 3CTQCh. 10 - Prob. 4CTQCh. 10 - Prob. 5CTQCh. 10 - Prob. 6CTQCh. 10 - a.Define the term RFLP. Explain how RFLPs are...Ch. 10 - Prob. 8CTQCh. 10 - Prob. 9CTQCh. 10 - Prob. 10CTQCh. 10 - Prob. 1CCCh. 10 - Prob. 2CCCh. 10 - Prob. 3CCCh. 10 - Prob. 4CCCh. 10 - From chapter 6, figure 6.19. What has happened to...Ch. 10 - Prob. 2VCCh. 10 - Using the words that follow, please create a...
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- How does cloning be helpful? Cite specific field or areas where cloning is beneficial. Explain in 10 sentences, please.arrow_forwardGenetics: Derive a formula for the number of copies that you should have in round x of a PCR given n copies at the startarrow_forwardExplain the Cloning a Gene? Define the different Forms of Cloning ?arrow_forward
- Briefly present experimental and practical benefits of using PCR in DNA cloning process. Give some examples of clinical applications.arrow_forwardDescribe the activities of the enzymes required to construct a recombinant DNA molecule.arrow_forwardExplain why PCR results in the amplification of a specific DNA sequence despite many competing sequencesarrow_forward
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