Microbiology: A Systems Approach
4th Edition
ISBN: 9780073402437
Author: Marjorie Kelly Cowan Professor
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 10, Problem 3MCQ
The function of ligase is to
- a. rejoin segments of DNA.
- b. make longitudinal cuts in DNA.
- c. synthesize cDNA.
- d. break down ligaments.
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The function of DNA ligase is to:
a. Catalyze formation of phosphodiester bonds between adjacent nucleotides
b. Catalyze formation of hydrogen bonds between adjacent nucleotides
c. Keep single strands of DNA apart during replication
d. Facilitate base pairing between single stranded molecules in DNA
e. Both a. and d. are correct
All of the following statements about telomerase are correct except:
A. the RNA component acts as a template for the synthesis of a segment of DNA.
B. it adds telomeric repeats to the 5'-ends of the DNA strands.
C. it provides a mechanism for replicating the ends of linear chromosomes.
D. it recognizes a G-rich single strand of DNA
E. it is a reverse transcripcase.
In nucleotide excision repair in E. coli, the function of the UvrA/UvrB complex is toa. detect DNA damage.b. make cuts on both sides of the damage.c. remove the damaged piece of DNA.d. replace the damaged DNA with undamaged DNA.
Chapter 10 Solutions
Microbiology: A Systems Approach
Ch. 10.1 - Provide examples of practical applications of...Ch. 10.2 - Prob. 2AYPCh. 10.2 - Describe how gel electrophoresis is used to...Ch. 10.2 - Prob. 4AYPCh. 10.2 - Prob. 5AYPCh. 10.3 - Prob. 6AYPCh. 10.3 - List examples of genetically modified bacteria,...Ch. 10.4 - Prob. 8AYPCh. 10.4 - Prob. 9AYPCh. 10.5 - Prob. 2CF
Ch. 10.5 - Outline in general terms the process of DNA...Ch. 10.5 - Prob. 11AYPCh. 10.5 - Prob. 12AYPCh. 10.5 - Prob. 13AYPCh. 10.6 - Prob. 14AYPCh. 10 - Prob. 1CFCh. 10 - Which of the following is/are not essential to...Ch. 10 - Prob. 2MCQCh. 10 - The function of ligase is to a. rejoin segments of...Ch. 10 - The creation of biological molecules entirely from...Ch. 10 - Which of the following sequences, when combined...Ch. 10 - A region of DNA in a plasmid that is recognized by...Ch. 10 - Prob. 7MCQCh. 10 - Which of the following is a primary participant in...Ch. 10 - Single nucleotide polymorphisms are found in a....Ch. 10 - Microarrays are used to monitor a. the rate of DNA...Ch. 10 - Prob. 11TFCh. 10 - A nucleic acid probe can be used to identify...Ch. 10 - Prob. 13TFCh. 10 - In order to detect recombinant cells, plasmids...Ch. 10 - Plasmids are the only vectors currently available...Ch. 10 - You are a public health official trying to...Ch. 10 - a.Construct a strand of complementary DNA (cDNA)...Ch. 10 - Prob. 3CTQCh. 10 - Prob. 4CTQCh. 10 - Prob. 5CTQCh. 10 - Prob. 6CTQCh. 10 - a.Define the term RFLP. Explain how RFLPs are...Ch. 10 - Prob. 8CTQCh. 10 - Prob. 9CTQCh. 10 - Prob. 10CTQCh. 10 - Prob. 1CCCh. 10 - Prob. 2CCCh. 10 - Prob. 3CCCh. 10 - Prob. 4CCCh. 10 - From chapter 6, figure 6.19. What has happened to...Ch. 10 - Prob. 2VCCh. 10 - Using the words that follow, please create a...
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- Which proteins are responsible for the unwinding of the double-stranded DNA during relication? A. Primases. B. Lyases. C. Helicases D. Topoisomerases E. Ligasesarrow_forwardWhen E. coli cells are mixed with recombinant vector DNA and subject to a stress such as heat shock, a small fraction of the cells will take up the plasmid DNA, a process known as : A. Ligation. B. Transformation. C. Transfection. D. Digestion.arrow_forwardMatch the activity below with the correct enzyme. (You won't use all the enzymes listed.) RNA acts as a template for DNA synthesis: RNA directs the cutting of an RNA molecule at a precise location: RNA directs the cutting of an DNA molecule at a precise location: options: a. Small Nuclear Ribonuclear Protein (SNRNP) b. telomerase c. primase d. helicase e. CRISPR/Cas9arrow_forward
- Match the correct terms with its definition A. Transcription B. Translation C. Amino acid D. Dehydration ..........1. Process of producing a polypeptide from mRNA. ..........2. Process that forms messenger RNA from a DNA gene. ..........3. RNA polymerase is continuous by adding nucleotides complementary to the DNA template. ..........4. Small ribosome subunit binds to mRNA, and tRNA enters the P active site on the ribosome. ..........5. Processes of monomers are linked together to synthesize a biological polymer. ..........6. The building blocks of protein.arrow_forwardPlace the steps of sanger sequencing in order.A. A fluorescent laser excites the fragments and records the wavelength consistent with a single nucleotide. B. ddNTPs bind and stop chain extension.C. DNA fragments are separated by size through a capillary tube. D. DNA polymerase copies the target region of template DNA.E. The final nucleotide of each fragment is labeled with a fluorescent tag.arrow_forwardWhich of the following is NOT true of DNA replication? a. Helicase uses ATP as an energy source. b. Primase uses ATP to build primers. c. Single-stranded binding proteins are enzymes that hold the strands open. d. Telomerase adds nucleotides to the original DNA template. e. Ligase uses a condensation reaction to make a single phosphodiester bond at each site of ligation..arrow_forward
- Transcription is initiated when: a. initiation factors assemble ribosomal subunits, mRNA, and initiator tRNA. b. RNA polymerase comes to a stop sequence. c. RNA polymerase binds to a region of DNA called the promoter. d. new nucleotides are added to an existing strand of nucleotides e. helicase unwinds the DNA templatearrow_forwardWould it be possible to start synthesizing the daughter DNA strand without assembling the RNA primer first? Why? Why not?arrow_forwardWhich of the following is true about DNA polymerase?a) It can synthesize DNA in the 5’ to 3’ directionb) It can synthesize DNA in the 3’ to 5’ directionc) It can synthesize mRNA in the 3’ to 5’ directiond) It can synthesize mRNA in the 5’ to 3’ directionarrow_forward
- The enzyme responsible for adding new nucleotides to a growing DNA chain during DNA replication is a. helicase. b. RNA polymerase. c. DNA polymerase. d. DNA ligase.arrow_forwardRNA can function: please explain your answer a.A) as a non-permanent template to synthesize protein b.B) to recruit the correct amino acid during translation c.C) as part of ribosomes to synthesize protein d.D) to help initiate DNA synthesis during replication e.Only A and C Only A, B and C A, B, C and Darrow_forwardDuring DNA replication: A. The two DNA strands separate, each strand then becomes a template for the assembly of a complementary strand. Each new DNA helix has one old strand with one new strand. B. The two DNA strands separate, each strand then becomes a template for the assembly of a similar strand. Each new DNA helix has one old strand with one new strand. C. The two DNA strands separate, each strand then becomes a template for the assembly of a similar strand. Each new DNA helix has two new strands. D. The two DNA strands separate, each strand then becomes a template for the assembly of an identical strand. Each new DNA helix has one old strand with one new strand.arrow_forward
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