Microbiology: A Systems Approach
4th Edition
ISBN: 9780073402437
Author: Marjorie Kelly Cowan Professor
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 10, Problem 14TF
In order to detect recombinant cells, plasmids contain antibiotic-resistance genes.
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You have set up a recombinant DNA experiment using the plasmid PBR322 as the vector (see plasmid below). You use the BamHI restriction site on the plasmid to insert the target DNA. The
plasmid is then used to transform E.coli colls Is the following statement True or False?
Growth of the transformed cells on agar containing both ampicillin and tetracycline will eliminate any cells that do not contain a plasmid.
Clal Hindlll
EcoRI
Pvul
BamHI
Pstl
amp
tet
PBR322
-Sall
ori
rop
Pvull
True
False
In bacterial transformation, the purpose of having antibiotic within an agar plate is to:
Select one:
confirm which plasmids been have successfully ligated with a gene of interest.
isolate bacteria which have been successfully transformed with the plasmid.
indicate which plasmids were successfully digested by the endonuclease.
act as a substrate which will be cleaved and produce a blue product when ligation is unsuccessful.
show which plasmids contain the lacZ gene.
Explain why recipient cells do not successfully takeup plasmids during natural transformation.
Chapter 10 Solutions
Microbiology: A Systems Approach
Ch. 10.1 - Provide examples of practical applications of...Ch. 10.2 - Prob. 2AYPCh. 10.2 - Describe how gel electrophoresis is used to...Ch. 10.2 - Prob. 4AYPCh. 10.2 - Prob. 5AYPCh. 10.3 - Prob. 6AYPCh. 10.3 - List examples of genetically modified bacteria,...Ch. 10.4 - Prob. 8AYPCh. 10.4 - Prob. 9AYPCh. 10.5 - Prob. 2CF
Ch. 10.5 - Outline in general terms the process of DNA...Ch. 10.5 - Prob. 11AYPCh. 10.5 - Prob. 12AYPCh. 10.5 - Prob. 13AYPCh. 10.6 - Prob. 14AYPCh. 10 - Prob. 1CFCh. 10 - Which of the following is/are not essential to...Ch. 10 - Prob. 2MCQCh. 10 - The function of ligase is to a. rejoin segments of...Ch. 10 - The creation of biological molecules entirely from...Ch. 10 - Which of the following sequences, when combined...Ch. 10 - A region of DNA in a plasmid that is recognized by...Ch. 10 - Prob. 7MCQCh. 10 - Which of the following is a primary participant in...Ch. 10 - Single nucleotide polymorphisms are found in a....Ch. 10 - Microarrays are used to monitor a. the rate of DNA...Ch. 10 - Prob. 11TFCh. 10 - A nucleic acid probe can be used to identify...Ch. 10 - Prob. 13TFCh. 10 - In order to detect recombinant cells, plasmids...Ch. 10 - Plasmids are the only vectors currently available...Ch. 10 - You are a public health official trying to...Ch. 10 - a.Construct a strand of complementary DNA (cDNA)...Ch. 10 - Prob. 3CTQCh. 10 - Prob. 4CTQCh. 10 - Prob. 5CTQCh. 10 - Prob. 6CTQCh. 10 - a.Define the term RFLP. Explain how RFLPs are...Ch. 10 - Prob. 8CTQCh. 10 - Prob. 9CTQCh. 10 - Prob. 10CTQCh. 10 - Prob. 1CCCh. 10 - Prob. 2CCCh. 10 - Prob. 3CCCh. 10 - Prob. 4CCCh. 10 - From chapter 6, figure 6.19. What has happened to...Ch. 10 - Prob. 2VCCh. 10 - Using the words that follow, please create a...
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- When using a conventional plasmid cloning vector containing a b-galactosidase gene, it is possible to perform a "blue-white screen" to determine which bacteria have taken up a plasmid into which a DNA fragment as been inserted, as opposed to those that have taken up just reclosed plasmid vector, by growing the transformed cells on nutrient agar plates containing the artificial b-gal substrate X-gal. Will bacteria that have taken up a plasmid into which a DNA fragment has been inserted form a blue colony or a white colony when grown on this medium? Briefly explain why these bacteria would form a colony of the color you chose.arrow_forwardWith reference to the image below, discuss the process and principle involved for screening/selection of hosts (last stage of cloning) containing the intended recombinant plasmid. LacZ' = Gene for alpha-peptide of β-galactosidase.arrow_forwardThe transfection reagent used to introduce plasmids to the HEK293 cells was a lipid reagent. Why is lipid utilized for this procedure?arrow_forward
- In the "Bacterial Transformation" experiment, why were there no fluorescence bacteria present in the other plates even though these were inserted by the +pGLO plasmid? (Note: Discuss the importance on the addition of ampicillin and arabinose to the medium of the genetically engineered bacteria). Limit your answer to 1-3 sentences only.arrow_forwarda) Where is the F plasmid located in an Hfr cell? Where is the F plasmid located in an F+ cell? b)arrow_forwarda) If lacZ was successfully inserted into the plasmid and E. coli successfully transformed, then the lacZ gene will be expressed which will breakdown X-gal producing a blue-colored colony. b) If the gene of interest was successfully inserted into the plasmid and E. coli successfully transformed, then the LacZ gene will not be expressed so X-gal will be broken down leading to a blue-colored colony. c) If the gene of interest was successfully inserted into the plasmid and E. coli successfully transformed, then the LacZ gene will not be expressed so X-gal will not be broken down leading to a white-colored colony. d) If lacZ was successfully inserted into the plasmid and E. coli successfully transformed, then the lacZ gene will be expressed which will breakdown X-gal producing a white-colored colony.arrow_forward
- Match the following Conjugation terms with the most appropriate description in the image: F- recombinant F- strain F+ strain HFR strainarrow_forwardYou want to propagate large amounts of a DNA fragment. To do this, use a plasmid vector. a) How should this plasmid vector be constructed? b) Also describe the function of the components.arrow_forwardWhat role did the transposon play in the transfer of resistance?arrow_forward
- Explain how you would use the blue-white colony screening technique to identify bacteria containing the recombinant plasmidarrow_forwardBriefly describe two different methods for inserting foreign DNA into plasmids, giving the strengths and weaknesses of each method.arrow_forwardUsing nucleotide letters, show the kind of cut that could be made on a DNA molecule to circularize it into a plasmid.What are restriction length polymorphisms, and how are they used?arrow_forward
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