Microbiology: A Systems Approach
4th Edition
ISBN: 9780073402437
Author: Marjorie Kelly Cowan Professor
Publisher: McGraw-Hill Education
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Question
Chapter 10.2, Problem 2AYP
Summary Introduction
To explain:
The role of restriction endonuclease in the process of genetic engineering.
Concept introduction
Recombinant DNA is defined as the joining of two different DNA from two different species and then the integrated DNA transformed into the host. This technology is termed as recombinant DNA technology.
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Explain what restriction enzymes are, how they function and how they can be used to make recombinant DNA
Explain how restriction enzymes recognize and digest DNA to create either blunt or sticky ends
b) Describe how DNA is digested by different restriction enzymes
c) Describe how gel electrophoresis is used to estimate the size of DNA fragments.
Chapter 10 Solutions
Microbiology: A Systems Approach
Ch. 10.1 - Provide examples of practical applications of...Ch. 10.2 - Prob. 2AYPCh. 10.2 - Describe how gel electrophoresis is used to...Ch. 10.2 - Prob. 4AYPCh. 10.2 - Prob. 5AYPCh. 10.3 - Prob. 6AYPCh. 10.3 - List examples of genetically modified bacteria,...Ch. 10.4 - Prob. 8AYPCh. 10.4 - Prob. 9AYPCh. 10.5 - Prob. 2CF
Ch. 10.5 - Outline in general terms the process of DNA...Ch. 10.5 - Prob. 11AYPCh. 10.5 - Prob. 12AYPCh. 10.5 - Prob. 13AYPCh. 10.6 - Prob. 14AYPCh. 10 - Prob. 1CFCh. 10 - Which of the following is/are not essential to...Ch. 10 - Prob. 2MCQCh. 10 - The function of ligase is to a. rejoin segments of...Ch. 10 - The creation of biological molecules entirely from...Ch. 10 - Which of the following sequences, when combined...Ch. 10 - A region of DNA in a plasmid that is recognized by...Ch. 10 - Prob. 7MCQCh. 10 - Which of the following is a primary participant in...Ch. 10 - Single nucleotide polymorphisms are found in a....Ch. 10 - Microarrays are used to monitor a. the rate of DNA...Ch. 10 - Prob. 11TFCh. 10 - A nucleic acid probe can be used to identify...Ch. 10 - Prob. 13TFCh. 10 - In order to detect recombinant cells, plasmids...Ch. 10 - Plasmids are the only vectors currently available...Ch. 10 - You are a public health official trying to...Ch. 10 - a.Construct a strand of complementary DNA (cDNA)...Ch. 10 - Prob. 3CTQCh. 10 - Prob. 4CTQCh. 10 - Prob. 5CTQCh. 10 - Prob. 6CTQCh. 10 - a.Define the term RFLP. Explain how RFLPs are...Ch. 10 - Prob. 8CTQCh. 10 - Prob. 9CTQCh. 10 - Prob. 10CTQCh. 10 - Prob. 1CCCh. 10 - Prob. 2CCCh. 10 - Prob. 3CCCh. 10 - Prob. 4CCCh. 10 - From chapter 6, figure 6.19. What has happened to...Ch. 10 - Prob. 2VCCh. 10 - Using the words that follow, please create a...
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- Describe two features that are characteristic of the action of restriction endonucleases. How do these features make restriction endonucleases useful to genetic engineers?arrow_forwardDescribe some general features of restriction sites.arrow_forwardOutline a series of steps by which reverse transcriptase produces DNA on an RNA template.arrow_forward
- Explain the role of reverse transcriptase in cloning.arrow_forwarda) Restriction enzymes are essential tools in genetic engineering. Discuss with diagrams b)Define the term genetic engineering and in a tabular form outline the differences between genetic recombination and genetic engineeringarrow_forwardDefine restriction endonuclease and explain what it does.arrow_forward
- Describe how the process of gene cloning results in a cell clonecontaining a recombinant plasmid.arrow_forwardDefine mutagen and describe how mutagens are usedin genetic research.arrow_forwardExplain how the presence of an antibiotic resistance gene can be used to determine whether a transformation has been successful.arrow_forward
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