You want to identify and compare the gene expression profile of several genes in a disease. Will you be able achieve this using Q-PCR technique?

Q: is possible to buy a CRISPR kit that you can do in your own home. Is this a good idea? After…

A: In 2013, the Zhang lab successfully published the first method to engineer CRISPR to edit the genome…

Q: An analyst designed a pair of primers to detect the presence of Alu sequence in human DNA genome…

A: PCR stands for a polymerase chain reaction. PCR is carried out under in-vitro or test tube…

Q: What color is the alpha gene spot on the microarray? Answer: Yellow please explain why is the…

A: The microarray can be described as a lab-on-a-chip. It can be used to detect the expression of…

Q: You are an expert molecular biologist and you just had your regular PCR run and you are now checking…

A: Following are the steps you will make to be able to avoid this in your next PCR run?   1. do the…

Q: Assuming that you start with one genome copy and are trying to amplify a 2000 bp DNA fragment, how…

A: PCR (Polymerase Chain Reaction) is a laboratory technique for amplifying a specific DNA segment. The…

Q: Select all that would be true if I had a missense mutation in an gene: The missense mutant protein…

A: A missense mutation is a type of point mutation in which a single nucleotide  change results in a…

Q: ou are amplifying both gst::egfp mRNA and 23S rRNA in the PCR steps. These are two different types…

A: The DNA in the nucleus makes different types of mRNA via transcription. Different types of mRNA have…

Q: BOTTOM The sequence depicted on the gel is 5'-GTGATGTAG-3' The sequence depicted on the gel is 3'-…

A: This method is used for DNA sequencing and it relies on the use of Deoxyribonucleoside…

Q: Aside from Sanger Sequencing, what are the other DNA sequencing methods that are available today?…

A: Sanger sequencing is a DNA sequencing technology that uses electrophoresis and is based on DNA…

Q: You would like to amplify the gene shown here using PCR.  Assume the primer to the 5' end of the…

A: PCR stands for Polymerase Chain Reaction. This is used to amplify the gene of interest.

Q: There are four possible observations that suggest an error occurred in a PCR reaction: a) Sequence…

A: PCR reaction is a method used to amplify a small sample into millions of small ones which help the…

Q: CRISPR techniques allow scientists to modify specific genes while sparing all others, thus…

A: Genetic diseases are the hereditary diseases that are very difficult to treat. With the advancement…

Q: Examine the DNA sequence shown below. You have been tasked with designing Primers for PCR…

A: Polymerase chain reaction (PCR) is a method used to  make millions of copies of a specific DNA…

Q: What is expected theoretical number of copies of DNA molecules after 28 cycles in a PCR experiment?…

A: INTRODUCTION: DNA : Its fullform is Deoxyribo nucleic acid and It has a double stranded helix…

Q: why are DTT and Mg added to PCRs? what is their affect and role in there?

A: The process of obtaining multiple copies of a known DNA sequence is known as gene amplification. It…

Q: 7. Based on the information provided, how many "cuts" would you expected after digesting the ligated…

A: The digestion of DNA with particular restriction enzyme is very critical process during the…

Q: (1) What is the major difference between the conventional PCR and real time PCR (qPCR) in terms of…

A: Question. What is the major difference between the conventional PCR and real time PCR (qPCR) in…

Q: How the Sequencing Technologies Have Progressed Rapidly ? Explain about this ?

A: Early efforts at sequencing genes ere troublesome, when Gilbert and Maxam reported the sequence of…

Q: What primers will be used to sequence your PCR product where are those sequences on your PCR product…

A: PCR is a technique used for amplification of DNA/gene of interest. At the end of the PCR technique…

Q: HI Molecule 2 Molecule 3 Legend: Thick black line - RNA. Thin black line - DNA. Thin dashed line -…

A: The polymerase chain reaction was given in 1985 by " Kary Mullis". It is a rapid in-vitro molecular…

Q: Following PCR amplification of a disease gene, you perform sequence analysis using a primer 20…

A: After performing sequencing through gel electrophoresis DNA fragments are organized according to…

Q: you wanted to design two primers to PCR amplify a gene from an organism that has 4 chromosomes, each…

A: In PCR that is, polymerase chain reaction two primers are needed  to hybridize with the sample DNA…

Q: You are working in a molecular biology laboratory and are having challenges with your PCR. You…

A: Polymerase chain reaction (PCR) is the method employed by the geneticist to multiply the number or…

Q: Imagine that you are producing a PCR product of approximately 2.1 kb in size with constant…

A: PCR: Represents the term polymerase chain reaction, and it helps to form more copies of a particular…

Q: Identify the mRNA sequence that encodes the protein Design primers that will allow them to amplify…

A: Deoxyribonucleic acid(DNA) is a molecule comprised of two polynucleotide chains coiled around each…

Q: Our PCR samples already contain loading dye, but sometimes this isn’t the case. If your samples…

A: Gel electrophoresis is a process that helps in the separation of DNA fragments based on sizes with…

Q: You are setting up your PCR reaction and accidentally add twice as much of the salt buffer as you…

A: Answer: PCR = Polymerase Chain Reaction is the technique used to amplify the strand of DNA using…

Q: 5'-GATTACAATAGGAATTC~~ GAATTCTCGTGACTCCG-3' 3'-CTAATGTTATCCTTAAG~ CTTAAGAGCACTGAGGC-5' Which of the…

A: PCR or polymerase chain reaction is a DNA amplifying technique where a short piece of a gene or a…

Q: Why RT-PCR is important in the sample preparation to perform expression microarray experiment?

A: RT - PCR : Reverse transcription- polymerase chain reaction is one of the types of PCR.  DNA…

Q: You would like to amplify the gene shown below using PCR with two primers: the primers are shown on…

A: Explanation: A specific area of DNA is amplified using PCR. Primers, which are compact fragments of…

Q: The following DNA sequence has been identified and named ‘Gene Z’. It is thought that this sequence…

A: PCR is a polymerase chain reaction, this is a biotechnological process which is used to amplify the…

Q: You are tasked with amplifying a gene of interest with PCR. a. After performing a Southern blot, you…

A: Polymerase Chain Reaction(PCR)  is a technique used to  amplify the number of DNA or RNA strands.…

Q: Primers can sometimes bind and target the wrong gene, especially if the primers are allowed to bind…

A: It is difficult for one to to find out gene of interest out of a complete genome. However, a given…

Q: please solve this with step-by-step calculations and explanations.

A: 1:

Q: You want to amplify a specific region of DNA during PCR. Your primers should

A: Primeras are short sequences of DNA with up to 18-20 base pairs composed of nitrogenous bases (…

Q: This is what the LAB FLOW would look like:                                   CULTURE BACTERIA…

A: PCR means polymerase chain reaction. Polymerase chain reaction is a method widely used to rapidly…

Q: let three loci be X,Y,Z. three pairs of primes can be used to amplify these loci in multiplex PCR.

A: STR are Short Tandem Repeats. These are the set of a few nucleotides which repeat themselves in a…

Q: If you were to isolate genomic DNA from two people and perform restriction enzyme digestion followed…

A: Agarose gel electrophoresis is a technique for distinguishing DNA bands based on size. To…

Q: PCR is a powerful technique to screen and amplify segments of DNA for use in recombinant protein…

A: Introduction PCR is a method for amplifying a specific region of DNA from a mixed template. Reagents…

Q: You are considering using restriction enzymes that recognizes 4-bp and 6-bp nucleotide sequences,…

A: From theory we know, the longer the restriction site recognized by a particular restriction…

Q: ou are an expert molecular biologist and you just had your regular PCR run and you are now checking…

A: Polymerase Chain Reaction PCR is an effective technique for producing large quantities of specific…

Q: What is the purpose of adding a primer to a PCR reaction? Is this primer made up of DNA or RNA…

A: The polymerase chain reaction is a method that is widely used to rapidly make millions of copies of…

Q: After running a qPCR experiment, we will have graphs showing the amount of fluorescence detected by…

A: The Polymerase Chain Reaction is a qualitative procedure which is used to produce large copies of…

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.