You are an expert molecular biologist and you just had your regular PCR run and you are now checking your PCR products if you have amplified the correct desired DNA fragment. Upon checking the results of the electrophoresis, you noticed that you have several bands in your gel. You expected that you will only have a band with the same size as with the "X" size of the DNA ladder. Unfortunately, you have also seen bands that fall in the "W", "Y" and "Z" markers of the ladder. Assume you got a very high quality DNA template from your extraction step. B. What are the steps you will make to be able to avoid this in your next PCR run?
You are an expert molecular biologist and you just had your regular PCR run and you are now checking your PCR products if you have amplified the correct desired DNA fragment. Upon checking the results of the electrophoresis, you noticed that you have several bands in your gel. You expected that you will only have a band with the same size as with the "X" size of the DNA ladder. Unfortunately, you have also seen bands that fall in the "W", "Y" and "Z" markers of the ladder. Assume you got a very high quality DNA template from your extraction step. B. What are the steps you will make to be able to avoid this in your next PCR run?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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You are an expert molecular biologist and you just had your regular PCR run and you are now checking your PCR products if you have amplified the correct desired DNA fragment. Upon checking the results of the electrophoresis, you noticed that you have several bands in your gel. You expected that you will only have a band with the same size as with the "X" size of the DNA ladder. Unfortunately, you have also seen bands that fall in the "W", "Y" and "Z" markers of the ladder. Assume you got a very high quality DNA template from your extraction step.
B. What are the steps you will make to be able to avoid this in your next PCR run?
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