You purchase a premix flour containing iron (Fe), riboflavin, niacin, thiamin, and folate. Fordetermination of Fe concentration in the flour sample, you conducted an atomic absorptionspectrometer (AAS) experiment using known concentration of Fe (standard). About 10 g offlour sample is used and mixed in 100 mL of solution then measured using AAS.1.20.80.60.40.281012-0.2Fe concentration (mg/L)[a] If the absorbance value of the flour sample is at 0.35, calculate the Fe concentrationpresence in the flour sample (obtained equation from the standard curve is y = 0.1018x -0.0514).[b] Commonly, the Fe contents are in the range of 1.6 mg to 2.3 mg in 100 g super flour tocomply with FDA's standard of identity. Based on your calculation, justify if the Feconcentration in the flour sample comply with the FDA specification.Absorbance at 248.3 nm

Atomic absorption spectrometry (AAS) is a simple, high-throughput, low-cost technique for analyzing…

Answered: You purchase a premix flour containing…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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You are given a 27 % (w/v) solution of psicose. Molar mass: psicose (C6H12O6) = 180.156 g/mol. ou have a 0.75 mg/mL solution of bovine serum albumin (BSA). You add 1 mL of the 0.75 mg/mL BSA solution into a standard cuvette (1 cm path length), set your spectrophotometer to 280 nm and read the absorbance of this BSA solution. Molecular mass of bovine serum albumin = 66.4 kDa. Molar extinction coefficient (e) of BSA = 43824 M-1 cm-1 (at 280 nm). What absorbance reading will you obtain?
Normal text Calibri BI UA G |EE| +2 I +3+1 CICCO4, 1 LE | ZJ VITICHI SUpstances did not test positive for any of the organic compound Distilled water did not test positive for any of the organic compounds. 5| 6 3) 4) What do all of the indicators you have used have in common? Indicators are all substances whose solutions change color due to changes in p 5) Is there a macromolecule that the tests in this lab did not test for No, there is no macromolecule that the lab did not test for. 6) Which compound is most common in foods that come from plants? Cellocuse is the single most common biochemical compound in living things. 7) If you wanted to reduce the amount of fat in your diet, what foods would you avoid? Steer away from foods high in saturated and trans fats like fried foods, fatty meats, stick margarine, snacks and items with hydrogenated oil. M 6 8) Which foods tested would your body use for a quick burst of energy? a. For energy when no carbohydrates are available? b. For building…
(b) Table Q1 below provides optical measurements of samples with known concentrations of analyte (ten samples were measured and the average and standard deviation are presented). They are also represented graphically in Figure Q1, below the table. Analyte concentration (nM) 0.1 0.2 0.5 1 2 5 10 15 fluorescence intensity 300000 250000 200000 150000 100000 50000 0 0 Table Q1 Fluorescence intensity (average) 9300 9200 10800 19600 37200 90000 178000 224000 5 [Analyte]/nM 10 Standard deviation 2000 1200 1000 2000 10000 30000 25000 40000 15 Figure Q1. Signal intensity for different analyte concentrations. Error bars are standard deviation. (ii) Calculate the limit of detection (LOD) for this sensor. (iii) A patient sample measured using this biosensor in the diagnostic laboratory generates a signal of 142800. The coefficient of variation of the device is 5%. Explain whether the diagnostic lab would have the confidence to report that the sample has a concentration in analyte of 8 nM or…
You need to make a 1/750 dilution of each human serum sample prior to testing it in your ELISA. You will need 1 ml of the diluted serum (which already includes extra for Murphy's Law.) Calculate the volume of undiluted human serum you need for your 1 ml test volume. 1.3 microliters O 13 microliters O 0.75 microliters O 7.3 microliters
Graph the data of the absorbance and starch concentration on Microsoft excel
The following were obtained when testing for carbohydrates. What is the type of carbohydrate present and what can you conclude from the results.
A paste of starch was tested using Benedict’s test, the Iodine Test, and Fehling’s Test. Before hydrolysis, Benedict’s test and Fehling's test results were both negative while Iodine’s test is positive. After adding 0.5 mL of conc. HCl to the paste, another 5 drops of NaOH were added and was tested using Fehling’s, Benedict’s Test, and Iodine Tests. What would be the theoretical result of the three different tests? Explain the chemical reaction that happened and the principle/mechanism behind the reactions.
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A 1:1000 dilution of 1mM NaCl would give: 100µM 1µM 1nM 10µM 1M 100mM 1mM 100nM 10mM
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make a schematic diagram of Iodine value determination using this data.

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