(b) Table Q1 below provides optical measurements of samples with known concentrations of analyte (ten samples were measured and the average and standard deviation are presented). They are also represented graphically in Figure Q1, below the table. Analyte concentration (nM) 0.1 0.2 0.5 1 2 fluorescence intensity 5 10 15 300000 250000 200000 150000 100000 50000 0 0 Table 01 Fluorescence intensity (average) 9300 9200 10800 19600 37200 90000 178000 224000 5 [Analyte] /nM 10 Standard deviation 2000 1200 1000 2000 10000 30000 25000 40000 15 Figure Q1. Signal intensity for different analyte concentrations. Error bars are standard deviation. (ii) Calculate the limit of detection (LOD) for this sensor. (iii) A patient sample measured using this biosensor in the diagnostic laboratory generates a signal of 142800. The coefficient of variation of the device is 5%. Explain whether the diagnostic lab would have the confidence to report that the sample has a concentration in analyte of 8 nM or whether it should only report it as positive.
(b) Table Q1 below provides optical measurements of samples with known concentrations of analyte (ten samples were measured and the average and standard deviation are presented). They are also represented graphically in Figure Q1, below the table. Analyte concentration (nM) 0.1 0.2 0.5 1 2 fluorescence intensity 5 10 15 300000 250000 200000 150000 100000 50000 0 0 Table 01 Fluorescence intensity (average) 9300 9200 10800 19600 37200 90000 178000 224000 5 [Analyte] /nM 10 Standard deviation 2000 1200 1000 2000 10000 30000 25000 40000 15 Figure Q1. Signal intensity for different analyte concentrations. Error bars are standard deviation. (ii) Calculate the limit of detection (LOD) for this sensor. (iii) A patient sample measured using this biosensor in the diagnostic laboratory generates a signal of 142800. The coefficient of variation of the device is 5%. Explain whether the diagnostic lab would have the confidence to report that the sample has a concentration in analyte of 8 nM or whether it should only report it as positive.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
![(b) Table Q1 below provides optical measurements of samples with known concentrations of
analyte (ten samples were measured and the average and standard deviation are presented).
They are also represented graphically in Figure Q1, below the table.
Analyte
concentration
(nM)
0.1
0.2
0.5
1
2
5
10
15
fluorescence intensity
300000
250000
200000
150000
100000
50000
0
0
Table Q1
Fluorescence
intensity
(average)
9300
9200
10800
19600
37200
90000
178000
224000
5
[Analyte]/nM
10
Standard
deviation
2000
1200
1000
2000
10000
30000
25000
40000
15
Figure Q1. Signal intensity for different analyte concentrations. Error bars are standard
deviation.
(ii) Calculate the limit of detection (LOD) for this sensor.
(iii) A patient sample measured using this biosensor in the diagnostic laboratory generates a
signal of 142800. The coefficient of variation of the device is 5%. Explain whether the
diagnostic lab would have the confidence to report that the sample has a concentration
in analyte of 8 nM or whether it should only report it as positive.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F0d77d484-a887-469d-aaae-779d8340e1ea%2F349e7a6a-06b6-42f8-975e-c163871dfc8d%2Fupesomv_processed.jpeg&w=3840&q=75)
Transcribed Image Text:(b) Table Q1 below provides optical measurements of samples with known concentrations of
analyte (ten samples were measured and the average and standard deviation are presented).
They are also represented graphically in Figure Q1, below the table.
Analyte
concentration
(nM)
0.1
0.2
0.5
1
2
5
10
15
fluorescence intensity
300000
250000
200000
150000
100000
50000
0
0
Table Q1
Fluorescence
intensity
(average)
9300
9200
10800
19600
37200
90000
178000
224000
5
[Analyte]/nM
10
Standard
deviation
2000
1200
1000
2000
10000
30000
25000
40000
15
Figure Q1. Signal intensity for different analyte concentrations. Error bars are standard
deviation.
(ii) Calculate the limit of detection (LOD) for this sensor.
(iii) A patient sample measured using this biosensor in the diagnostic laboratory generates a
signal of 142800. The coefficient of variation of the device is 5%. Explain whether the
diagnostic lab would have the confidence to report that the sample has a concentration
in analyte of 8 nM or whether it should only report it as positive.
Expert Solution
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
Step by step
Solved in 3 steps with 2 images
Recommended textbooks for you
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON