- Cryoscopic method - White-Vincent Method - E method - Sprowl's method   It is the most widely used method in adjusting tonicity by the amount of sodium chloride.   This method expresses the freezing point in terms of 1% solution.   This method uses a constant that denotes the volume of isotonic preparation in milliliters when 1 gram of sodium chloride is dissolved in water.   This method uses a constant of 0.3 to denote the weight of the drug.   This method uses the equation "V = 33.33E

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