The standard curve to determine the amount of betacyanin is shown below. You extracted a red pigment from a beet disc (the mass of a disc is 2 g) using 10 ml of 20% ethanol solution. You measured absorbance of the solution above the beet disc every minute for a total time of 20 minutes. The increase in absorbance was linear during a period of time from 1 min to 10 min. The absorbance at 10 min was 0.8. Calculate the amount of betacyanin extracted from 1g of a beet tissue per minute. Explain your calculations. You can use Excel or a calculator.

The standard curve to determine the amount of betacyanin is shown below. You extracted a red pigment from a beet disc (the mass of a disc is 2 g) using 10 ml of 20% ethanol solution. You measured absorbance of the solution above the beet disc every minute for a total time of 20 minutes. The increase in absorbance was linear during a period of time from 1 min to 10 min. The absorbance at 10 min was 0.8. Calculate the amount of betacyanin extracted from 1g of a beet tissue per minute. Explain your calculations. You can use Excel or a calculator.

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

See similar textbooks

Similar questions

You have been given a stock solution of dye that has a 10X concentration. You wish to make 750ul of a 1X dilution of this dye. Give the volume of 10X dye that you will use (in ul)
Calculate the appropriate volume (in µL) of 9X loading buffer that should be added to 46.0 µL of a sample prior to loading the sample on an agarose gel.
Explain the color changes observed throughout the peroxide value of oil experiment. Materials:1. Old and fresh oil samples2. Distilled water3. Acetic acid/chloroform solution4. Saturated potassium iodide solution5. Sodium thiosulphate (0.01M)6. 1% Starch indicator: dissolve 1g of starch in 10 ml of water and then add 90 ml of hotwater to it
A vial of Doxorubicin reads 0•5g per vial. Instructions say to reconstitute each 12mg with 2•5ml of NS. How many ml of NS will be needed to reconstitute the vial of the recommended concentration? please show working
Laemmli buffer, also referred to as SDS-reducing buffer, is added to each of the samples. It is a blue solution and the recipe for 2X Laemmli buffer is: 0.125 M Tris HCl, pH 6.8, 20% glycerol, 4% SDS and 10% β-mercaptoethanol (βME). What is the purpose of each of these ingredients?
If a powder in a 2gm vial diluted with 6.8ml of diluent provides 8ml of solution and each ml contains 250mg of a drug, how many ml is needed to give 750mg.
Consider a pH titration curve at 298 K for 50 mL of 0.10 M hypobromous acid, HBrO (Ka = 2.8 x 10−9), titrated with 0.10 M KOH solution. a. What volume of KOH is needed to reach the half-equivalence point? b. Calculate the pH of the HBrO sample at the half-equivalence point. c. Calculate the equivalence point pH. d. Identify a satisfactory pH indicator for the equivalence point. The pH range of colour change is indicated in parentheses. A) Methyl orange (3.0 - 4.3) B) Bromocresol purple (5.0 - 6.5) C) Alizarin yellow R (10.0 - 12.0) D) Methyl red (4.2 - 6.2)
Consider the fluorescence spectra for bovine serum albumin (BSA) below. One solution contains phosphate buffered saline (PBS), while the other contains 1M HCL. Why does the fluorescence change the way it does? I.e., why do we observe a higher peak and a shift to the left under the presence of acid?
Calculate the amount of phycocyanin in Sample 1 in mg where A620=0.192 and A650=0.090, taking into account the dilution factor (100), and the total volume of extract (45). Note your answer to 2 decimal places.
You resuspend 1 ml of of a purified chlorophyll sample in 10 ml of 80% acetone, and determine that the optical density of the solution (at 652 nm) is 0.88. What is the concentration of your original preparation?
in food microbiology, how do you compute for concentration (M) and absorbance (A) of peroxidase activity on hydrogen peroxide at different pH values? (wavelength used = 415nm). can you please explain the calculations thank you if given: Temperature = 75 C Molar absorptivity coefficient = 10.5 Path length (cm) = 2 I = transmitted light = 0.45
Most of the following conclusions regarding the photo shown above are correct Except: All of the following are correct regarding the disk diffusion test photos EXCEPT : Zone of inhibition Chlorine Chlorine Chlorine O-phenylphenol Hexachlorophene O-phenylphenol Hexachlorophene Hexachlorophene O-phenylpheno Quat Quat Quat Staphylococcus aureus (gram-positive) Escherichia coli Pseudomonas aeruginosa (gram-negative) (gram-negative) Select one: O to. Gram negative bacteria are the most sensitive to chemical agents O b. Only one of the four chemicals affected Pseudomonas O c. Gram positive bacteria are the most sensitive to disinfectants O d. Hexachlorophene was effective only against Gram positive bacteria. O and. Chlorine was effective against all bacteria bacteria tested