The significance of using immersion oll when using 100X objective lens is: It will increase the contrast by having same refractive index as the lens, loss of light by refraction, reflection and diffraction is minimized . the specimens need not be killed, fixed and stained more light is captured for better illumination, allowing the specimen to fluoresce 1. il. ii. iv.
The significance of using immersion oll when using 100X objective lens is: It will increase the contrast by having same refractive index as the lens, loss of light by refraction, reflection and diffraction is minimized . the specimens need not be killed, fixed and stained more light is captured for better illumination, allowing the specimen to fluoresce 1. il. ii. iv.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:The significance of using immersion oil when using 1OOX objective lens is
The significance of using immersion oll when uskng 100X objective lens is:
It will increase the contrast
by having same refractive index as the lens, loss of light by refraction, reflection
and diffraction is minimized.
the specimens need not be killed, fixed and stained
more light is captured for better illumination, allowing the specimen to fluoresce
i.
il.
iii.
iv.
A light microscope should have the following features
contrast
Resolution
magnification
fluorescence
ii.
iv.
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