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Louie performed paper chromatography to identify which among the four available reference mixtures - WAR, OFF, YIN, MEW - have the same components as his unknown sample, GUN. He spotted the standards and the sample in a Whatman No. 1 filter paper, which he placed in a developing chamber containing 3% KCI. After 20 minutes, Louie obtained the chromatogram below, immediately noting that the distance travelled by the solvent from the origin is 79.0 mm. On the other hand, the distances (in cm) traveled by the developed spots were tabulated as follows.

Louie performed paper chromatography to identify which among the four available reference mixtures - WAR, OFF, YIN, MEW - have the same components as his unknown sample, GUN. He spotted the standards and the sample in a Whatman No. 1 filter paper, which he placed in a developing chamber containing 3% KCI. After 20 minutes, Louie obtained the chromatogram below, immediately noting that the distance travelled by the solvent from the origin is 79.0 mm. On the other hand, the distances (in cm) traveled by the developed spots were tabulated as follows.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Louie performed paper chromatography to identify which among the four available reference mixtures – WAR, OFF, YIN, MEW – have the same components as his unknown sample, GUN. He spotted the standards and the sample in a Whatman No. 1 filter paper, which he placed in a developing chamber containing 3% KCI. After 20 minutes, Louie obtained the chromatogram below, immediately noting that the distance travelled by the solvent from the origin is 79.0 mm. On the other hand, the distances (in cm) traveled by the developed spots were tabulated as follows. solvent front F B H E I A C origin WAR OFF ΥIN MEW GUN DISTANCE DISTANCE TRAVELLED FROM SPOTS TRAVELLED FROM SPOTS THE ORIGIN, cm THE ORIGIN, cm A 2.70 F 5.40 В 4.75 G 2.90 2.25 3.50 C 4.10 D I 2.85 E 3.25 4.95 1. Is the type of paper chromatography performed normal or reversed? Why?
Transcribed Image Text:Louie performed paper chromatography to identify which among the four available reference mixtures – WAR, OFF, YIN, MEW – have the same components as his unknown sample, GUN. He spotted the standards and the sample in a Whatman No. 1 filter paper, which he placed in a developing chamber containing 3% KCI. After 20 minutes, Louie obtained the chromatogram below, immediately noting that the distance travelled by the solvent from the origin is 79.0 mm. On the other hand, the distances (in cm) traveled by the developed spots were tabulated as follows. solvent front F B H E I A C origin WAR OFF ΥIN MEW GUN DISTANCE DISTANCE TRAVELLED FROM SPOTS TRAVELLED FROM SPOTS THE ORIGIN, cm THE ORIGIN, cm A 2.70 F 5.40 В 4.75 G 2.90 2.25 3.50 C 4.10 D I 2.85 E 3.25 4.95 1. Is the type of paper chromatography performed normal or reversed? Why?
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