You obtained the following raw data when setting up a Bradford standard curve: BSA (mg/ml) Absorbancy 595nm 0.225 0.310 0.420 0.510 0.610 0.720 0.810 0.915 8. 0.950 0 980 0.990 After blanking against a bradford-dH20 sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.679 was ob Set up a standard curve, ekeluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant fiqures) o12 3456 7091
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- You obtained the following raw data when setting up a Bradford standard curve: BSA (mg/ml) Absorbancy 595nm 0 0.225 1 0.310 2 0.420 3 0.510 4 0.610 5 0.720 6 0.810 7 0.915 8 0.950 9 0.980 10 0.990 After blanking against a bradford-dH2O sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.570 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant figures).You obtained the following raw data when setting up a Biuret standard curve: BSA (mg/ml) Absorbancy 540nm 0 0.158 1 0.210 2 0.260 3 0.305 4 0.360 5 0.410 6 0.455 7 0.510 8 0.530 9 0.550 10 0.554 After blanking against a biuret-dH2O sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.262 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant figures).You obtained the following raw data when setting up a Biuret standard curve: Absorbancy BSA (mg/ml) 540nm 0 0.158 1 0.210 2 0.260 3 0.305 4 0.360 5 0.410 6 0.455 7 0.510 8 0.530 9 10 0.550 0.554 After blanking against a biuret-dH20 sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.284 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg/ml (up to 3 significant figures).
- Using a P-1000 micropipette, you transfer 800 microliter of ddH2O to a beaker and measure its weight. It turns out to be only 780 milligram; what is the percent error of this micropipette?Match the number in the image with the correct name for the lab supply 1 2 ♡ AGEN QIAGEN QIAGEN QIAC RIVCEM БИЗӘДІС RNeasy Mini Kit (50) Cat. No. 74104 Store at room temperature (15-25°C) WEZAIO QIAGEN 2 GDIVERM QIAGL K (20) QIAGL умени DAIO QIAG GAGEN GIVCEN QIAGEN QIAGEN ИЗОМО QIAGEN NEA QIAGEN QIAGEN N QIAGEN QIAGE Buffer W AGEN Lysis buffer 45 m Buffer RPE Wash buffer 11 ml concentrate to ustan 5 m Butter R U 3 clear collection tube centrifuge tube pink column (with filter) PhaseLock tube pink column (with filter) inside a clear collection tube <The concentration of hydrolyzed nitrocefin at each time point for an experiment is given below. Time (min) Concentration (μM) 0.5 6.01 1 11.78 1.5 17.6 2 23.51 2.5 29.58 3 35.31 3.5 39.73 4 44.5 4.5 48.18 5 50.05 5.5 52.72 6 54.01 6.5 55.06 7 55.65 7.5 56.39 8 56.74 8.5 57.49 9 58.03 9.5 58.61 10 58.69 Make a graph that plots the concentration of hydrolyzed nitrocefin (in μM) against time (in seconds) using Excel, R, SPSS or other computable software. Where appropriate, include a trendline that shows the linear range on your graph. Include the equations for the trendlines and the R2 value on the graph. Your graph should also include a title and appropriate titles for the x- and y-axes, with units included where appropriate. To determine the initial velocity of a possible insert in this experiment, you must determine what the linear range is in these data (see note below). Based on your graph, which time points represent a suitable linear…
- Solution Absorbance mg/ml aspirin Standard solution - 1.6 mg/mL A 0.638 0.08 mg/mL B 0.504 0.064 mg/mL C 0.376 0.048 mg/mL D 0.259 0.032 mg/mL E 0.126 0.016 mg/mL A = -log T where T = %T ÷ 100 Construct a callibration curve using the above data. Absorbance should be on the vertical axis and "mg/mL of acetylsalicylic acid" on the horizontal axis. The line should go through the origin. Using the data provided, the graph you have generated, and the procedure that was used to generate the solutions which were examined by spectroscopy, calculate the amount of acetylsalicylic acid per tablet. Commercial tablet 1 labelled as 100 mg enteric coated Absorbance = 0.16 Commercial tablet 2 labelled as 300 mg Absorbance = 0.45 Student prepared tablet from practical 5 Absorbance = 0.19 Using the data provided, the graph you have generated, and the procedure that was used…From the following data, find the % (w/w) cream of tartar (KHC4H406, MM=188): Wt of sample = 1.4160 g NaOH titrant used = 20.87 mL HCI used for back titration = 1.27 mL 1.00 mL HCI 1.12 mL NaOH 1.00 mL HCI A 0.01930 g CaCO3 89.08% (w/w) 87.12% (w/w) O 47.75% (w/w) O 95.59% (W/w)What is the experimentally-determined minimum inhibitory concentration of the antibiotic being tested in this picture, based on the results shown in the picture? 0.06 0.125 0.25 1.0 μg/ml 8.0 µg/ml 0.5 µg/ml ○ 0.06 µg/ml µg/ml 0.5 1.0 2.0 4.0 8.0
- Calculate the volume of BSA stock that will be required to make the standard solutions needed to create the BSA standard curve. Be sure to show your work and include the volume of 0.02 M phosphate buffer required to reach a final volume of 1 mL. From a 2,000 μg/mL BSA stock, create 1 mL of each of the following stock solutions in 0.02 M phosphate buffer using individual microcentrifuge tubes: 50 μg/mL, 250 μg/mL, 500 μg/mL, 1,000 μg/mL, 1,250 μg/mL, 1,500 μg/mL. Be sure to properly label all the microcentrifuge tubes before creating the standards.KCI for injection contains 40 mEq in 20 mL. An order requires 15 mEq, how many mL are needed?A mixture containing only KCl and NaBr is analyzed by the Mohr Method. A 0.3172-g sample is dissolved in 50 mL water and titrated to the Ag2CrO4 endpoint, requiring 36.85 mL of 0.1120 M AgNO3. A blank titration requires 0.71 mL of titrant to reach the same endpoint. Report the % (w/w) KCl and NaBr in the sample. [Ans . 84.41 % (w /w); 17.59 % (w /w)]