5. Coomassie Brilliant Blue G-250, a dye used in the Bradford assay, has a maximum absorbance at 595 nm under acidic pH. 6. The graph of solubility vs pH results in a vertical parabola with a maxima near the sample's isoelectric pH. 7. Coomassie Brilliant Blue, a reagent or standard that is usually used to determine the void volume of a gel filtration chromatography column, has an approximate molecular weight of 2.000.000 Da.

5. Coomassie Brilliant Blue G-250, a dye used in the Bradford assay, has a maximum absorbance at 595 nm under acidic pH. 6. The graph of solubility vs pH results in a vertical parabola with a maxima near the sample's isoelectric pH. 7. Coomassie Brilliant Blue, a reagent or standard that is usually used to determine the void volume of a gel filtration chromatography column, has an approximate molecular weight of 2.000.000 Da.

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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