You isolate nuclei from three different eukaryotic species. You treat the samples in exactly the same way (adding same amount of enzyme, buffer and time) to partially digest the chromatin with micrococcal nuclease, separate the DNA from the protein, and run the DNA on an agarose gel. You see the pattern below: Lane 1 2 3 Approximate size of bands (in base pairs) 200, 400, 600, 800 180, 360, 540, 720 190, 380, 570, 760 Knowing that core-DNA in all cell types is the same what is your explanation for the difference in DNA size in the patterns you observe?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
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You isolate nuclei from three different eukaryotic species. You treat the samples in exactly the same way
(adding same amount of enzyme, buffer and time) to partially digest the chromatin with micrococcal
nuclease, separate the DNA from the protein, and run the DNA on an agarose gel. You see the pattern
below:
Lane
1
2
3
Approximate size of bands (in base pairs)
200, 400, 600, 800
180, 360, 540, 720
190, 380, 570, 760
Knowing that core-DNA in all cell types is the same what is your explanation for the difference in DNA
size in the patterns you observe?
Transcribed Image Text:You isolate nuclei from three different eukaryotic species. You treat the samples in exactly the same way (adding same amount of enzyme, buffer and time) to partially digest the chromatin with micrococcal nuclease, separate the DNA from the protein, and run the DNA on an agarose gel. You see the pattern below: Lane 1 2 3 Approximate size of bands (in base pairs) 200, 400, 600, 800 180, 360, 540, 720 190, 380, 570, 760 Knowing that core-DNA in all cell types is the same what is your explanation for the difference in DNA size in the patterns you observe?
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