You have retrieved your MHA (Mueller-Hinton agar) plate, your materials, and your E. coli culture.  What is the first step to prepare your Kirby-Bauer test?

Q: Is the Triple-Sugar Iron Agar (TSIA) a complex or defined medium? Explain based on its composition.…

A: Introduction A solid or liquid substrate that can be used to grow cells or organ explants; a medium…

Q: In the ELISA, the pH the coating buffer was 9.6 whereas the pH of the sample buffer (PBS based) was…

A: Coating buffer in ELISA is a mix of sodium carbonate and bicarbonate at ph 9.4. This gives charge to…

Q: Why are biochemical tests used to identify microbes?

A: Microbes are living organisms that are very small and can be seen only with the help of a…

Q: 1) What is a positive and negative results for Antibiotic sensitivity test: The Kirby-Bauer…

A: An antibiotic sensitivity test, also known as an antibiotic susceptibility test, is a laboratory…

Q: If coliforms were present on a plated sample, would you know with certainty that the coliforms are…

A: Coliform microbes are characterized as rod-shaped, gram-negative, non-spore-forming, and…

Q: Hello, I want you pleaseeee to make a dichotomous key based on provided experiments to identify a an…

A: The method of identifying an unknown bacterium is a precise one, requiring cautious perception and…

Q: identify the organisms in  an unknown culture. You find that the culture contains two gramnegative…

A: A culture is a medium to grow bacteria and other microorganisms  It provides the bacteria with…

Q: Koch's postulates include all of the following EXCEPT O 1) Isolate the organism in pure culture. The…

A: Koch's postulates are a series of findings and experimental criteria suggested in the late 1800s by…

Q: You are interested in obtaining Staphylococcus aureus for a study investigating the prevalence of…

A: Staphylococcus aureus is highly prone for antibiotic resistance. Therefore while handling these…

Q: Why is it best to use sterile distilled water in the preparation of microbial suspension and dry…

A: Smear preparation is the first step carried out during the staining procedure.

Q: A patient sample will be analyzed. You are responsible to quantify the bacteria number. The dilution…

A: A sample of your blood, urine, skin, or any part of your body will be taken during a bacteria…

Q: thanol has been carried over into your DNA sample (two answers possible here, select both of them)…

A: DNA is not soluble in ethanol . But when ethanol is there DNA gets separated by forming white…

Q: You perform the Methyl-Red(MR)& Voges-Proskauer(VP)test and gather the followingresults:…

A: Since you have asked multiple questions, we will solve the first question for you. If you want any…

Q: List the following reagents in the order in which they are added to an ELISA: secondary antibody,…

A: ELISA stands for enzyme linked immuno sorbent assay. It is used for identifying the antigen present…

Q: If a technician examined a Kirby-Bauer assay and found individualcolonies of the plated microbe…

A: Prokaryotes are the primitive organisms that were the first to develop and harness life on Earth.…

Q: Match the confirmation test to the category of bacteria. (each category can be used multiple times)…

A: Gram-positive bacteria are bacteria that test positive in the Gram stain test, which has…

Q: What is the importance of the Nitrate test? What information can we gather from this test? Please…

A: Nitrate broth used in the nitrate test contains nutrients and potassium nitrate as a source of…

Q: On the basis of the appearance of this place, what are you testing for? When you added a reagent to…

A: Catalase is an enzyme that reacts with hydrogen peroxide to form water and oxygen. Catalase enzyme…

Q: Please discuss how the Methyl Red-Vogues Proskauer test helps distinguish microbes from each other.

A:

Q: Mueller-Hinton agar needs to be made to specific standards in order to yield reliable, reproducible…

A:

Q: What biochemical tests did we use to distinguish staphylococci from streptococci?

A: Staphylococcus and streptococcus both occur in cocci shape and both are gram positive organisms.…

Q: Once the completed test establishes the presence of coliforms in the water sample, why might you…

A: Water is tasteless, transparent, inorganic. Colorless, and odorless chemical substance. Its chemical…

Q: How would you produce a 10^-1 dilution if a 3 mL bacterial sample using the entire 3 mL volume?…

A: Dilution factor refers to the ratio of the volume of the initial (concentrated) solution to the…

Q: Why is it necessary to inoculate the KIA medium all the way down to the bottom of the tube during…

A: KIA (Kligler Iron Agar) medium is a differential and selective medium used in microbiology to…

Q: You were instructed to add 1.0ml out of 4.0ml of an undiluted sample to 99ml of sterile diligent you…

A: Dilution factor is the ratio of volume of the initial (concentrated) solution to the volume of final…

Q: What kinds of organisms may be plentiful in a milk sample, yet give a negative reductase test?

A: Methylene blue reduction test is based on the principle of oxygen reduction by increased microbial…

Substance Abuse

Substance abuse refers to the use of illegal drugs in excessive quantities, as well as the misuse of legal substances such as prescription or over-the-counter pharmaceuticals or alcohol for purposes other than those intended. Drug addiction, also known as substance use disorder, is a disease that affects the brain and behavior of a person. It leads to a failure to control the use of any drug or prescription, legal or illegal.