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If coliforms were present on a plated sample, would you know with certainty that the coliforms are disease-causing? Why or why not?
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- 2) A pure culture A) is sterile. B) is a population of identical cells. C) is made of a clearly defined chemical medium. D) contains one microbial cell.Why is aseptic urine collection important when cultures are ordered? If you counted 20 colonies from a 0.01-ml inoculum of a 1:10 dilution of urine, how many organisms per milliliter of specimen would you report? Is this number significant? What can you learn from visual inspection of a urine specimen? How would you relate these to the microorganisms present in the sample? How are UTIs acquired/transmitted? Explain why E. coli is frequently implicated in cystitis in females.You plated 1.0 mL of a sample diluted by a factor of 10^-5 and you counted 50 comonies after incubation. The concentration of bacteria in the stock culture is: A) 5x10^5 B) 5x10^4 C) 50x10^4 D) 50x10^-6 E) 5x10^-4
- On the basis of the appearance of this place, what are you testing for? When you added a reagent to this plate, these bubbles appeared. What is the name of this reagent and what are those bubbles?There are so many microbes in a single mL of culture, it is very difficult to perform one dilution to produce countable cells. Microbiologists need to perform a dilution series, where multiple dilutions are performed in sequence to arrive at the correct dilution. Dilutions are cumulative. Multiple the series of dilutions together to find the final dilution value. If 3 serial dilutions are performed, each with a value of 0.01, what is the cumulative dilution? Express your answer as an exponent, e.g. 0.1 would be 1e-1 and 0.01 would be 1e-2You mixed up the numbers on the tubes when you inoculated the mannitol salt agar (MSA) plate. You do not know if you grew staph epidermis or E. coli. You found that the organism growing on the mannitol salt agar remained red after incubation. It is most likely that the organism is E.coli. a) True b) False
- If no amplified product was visualized by UV light on the gel, does it mean that there is no water-borne microbial pathogen in the unknown sample?If each agar plate contained 20 ml of LB agar and had an ampicillin concentration of 150 ug/ml, what is the approximate amount of ampicillin (grams) in each plate?In performing the Kirby-Bauer procedure in a clinical laboratory setting, why must the agar be a certain depth? Why must the absorbance of the inoculum be standardized?
- Placing a 1 ml sample of raw milk into a 99ml sterile water, then transferring a 1ml sample from that dilution to another 99ml sterile water produces a ___________ dilution. a) 1/100 b) 1/10 c)1/100,000 d)1/1000 e)1/10,000You spread 0.1 mL volume of a 10^(-6) dilution onto a nutrient agar plate. After 24 hours of incubation at 37°C, there were 280 colonies of bacteria on the plate. A.) What is the original concentration (OCD) of bacteria in the stock sample this dilution came from? B.) Using the OCD value from part A, determine the number of colonies that would be expected to grow on a plate that is inoculated with 0.1 mL volume of 10^(-8) dilution from this same stock of bacteria. Show your work for both.You spread 0.1 mL volume of a 10^(-6) dilution onto a nutrient agar plate. After 24 hours of incubation at 37°C, there were 280 colonies of bacteria on the plate. A.) What is the original concentration (OCD) of bacteria in the stock sample this dilution came from? B.) Using the OCD value from part A, determine the number of colonies that would be expected to grow on a plate that is inoculated with 0.1 mL volume of 10^(-8) dilution from this same stock of bacteria.