What is the coliform test when is it used? What is its purpose? How does it function? What are the advantages and disadvantages of utilizing the coliform test?

Q: What is the principle of freezing of cell lines and primary cells.Describe the general protocol for…

A: Cell culture is a procedure in which cells (either animal or plant cells) are separated from an…

Q: What is the role of H2SO4 in Hopkins cole test?

A: The Hopkin's Cole test, sometimes known as the 'glyoxylic acid test,' is a method for detecting the…

Q: What are the pros and cons of serial dilution method and W/V % method?

A:

Q: Why are biochemical tests used to identify microbes?

A: Microbes are living organisms that are very small and can be seen only with the help of a…

Q: What is the result of tube A in this Voges-proskauer test? What are the reagents of this test? What…

A: Voges-proskauer test is performed to differentiate between facultative anaerobic enteric bacteria.

Q: What is the chemical basis of the Xanthoproteic Test? Give the chemical reaction involved.

A: Protein is a nitrogenous organic macromolecule that is essential to human health. It is responsible…

Q: Record the zones of inhibition in the chart above. Which, if any, of the drugs would you prescribe…

A: Antimicrobial agents are compounds used in order to treat infectious diseases as they have the…

Q: What are the steps in sandwich ELISA? Explain and provide a schemiatic diagram

A: Enzyme immunoassay (EIA) and enzyme-linked immunosorbent assay (ELISA) are both widely used as…

Q: The following pictures show the results of a Disk Diffusion Assay for different types of bacteria.…

A: Disk diffusion assay is a laboratory test used to determine the effectiveness of antimicrobial…

Q: Discuss the correct laboratory technique in using the centrifuge for qualitative analysis. Give two…

A: In labs, a centrifuge is a vital instrument that isolates diverse parts of a mixture, like fluids…

Q: What is an E-test strip, and what is it used for in the microbiology laboratory? Draw a diagram of…

A: Microbiology is a branch of science which deals with the study of microscopic organisms such as…

Q: If the 10-9 plate was counted to have 155 colonies on it, would the CFU/mL be 1.55*1011? If not,…

A: CFU  Colony forming unit it a method to calculate the number of microorganisms in a culture media.

Q: What are ELISA assays used for in labs? Give at least three examples.

A: ELISA - Enzyme-linked immunosorbent assay ELISA is an analytical immunological technique to detect…

Q: What is the principle behind invertase test

A: Invertase was an enzyme that hydrolyses the disaccharide sucrose into monosaccharides glucose and…

Q: What is optical density (OD) and how is it measured? How is it related to the concentration of the…

A: UV–vis spectrophotometric optical density (OD) is the most commonly-used technique for estimating…

Q: What is the importance of the Nitrate test? What information can we gather from this test? Please…

A: Nitrate broth used in the nitrate test contains nutrients and potassium nitrate as a source of…

Q: What is the principle of pettenkofer's test in detetecting the presence of bile salt in the urine…

A: The chief components of bile juice are the bile salts. Bile is a pigmented fluid having the color…

Q: What is an advantage of using an ELISA instead of a protein microarray to study a proteome? What is…

A: ELISA is an immunological assay commonly used to measure antibodies, antigens, proteins and…

Q: in an indirect elisa procedure what enzyme is used?

A: Enzyme-linked immunosorbent assay (ELISA) is an assay technique used for detecting and quantifying…

Q: cal is utilized as the reagent known as VP-B?

A: The Voges-Proskauer (VP) test is generally used to test if an organism produces acetyl methyl…

Q: The following pictures show the results of a Disk Diffusion Assay for different types of bacteria.…

A: Antibiotics Antibiotics are the drugs used to treat bacterial infections. They may be bactericidal (…

Q: What are the tests to determine the level of antimicrobial activity of a antimicrobial agent? How…

A: Antibiotic disc diffusion study is also known as the Kirby-Bauer method in which the susceptibility…

Q: Name three reasons why the ELISA test is useful in detecting microbes

A: ELISA:            ELISA ~ Enzyme-Linked Immunosorbent Assay                   A screening tool used…

Q: Describe the mechanism of an Indirect ELISA. Why is ELISA so sensitive? Why is it necessary to block…

A: The enzyme linked immunosorbent assay (ELISA) is an analytical biochemistry assay which uses the…

Q: In ELISA, what is the importance of washing? When does washing is performed?

A: Introduction :- Enzyme-linked immunoassay (ELISA) is an acronym for enzyme-linked immunoassay.…

Q: escribe the examples of biochemical test that are used to identify the bacteria in the laboratory.

A: According to the definition of a biochemical test, it is a test that is used to distinguish between…

Q: What is the role of the substrate? Is it really necessary to add substrates in ELISA? Why, or why

A: * ELISA is called as enzyme linked immunoassay which is used to detect antibodies in blood. *An…

Substance Abuse

Substance abuse refers to the use of illegal drugs in excessive quantities, as well as the misuse of legal substances such as prescription or over-the-counter pharmaceuticals or alcohol for purposes other than those intended. Drug addiction, also known as substance use disorder, is a disease that affects the brain and behavior of a person. It leads to a failure to control the use of any drug or prescription, legal or illegal.